Method for detecting mold resisting properties of bacterium resisting leather by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting the mold resisting properties of leather. The method comprises the detection steps of preparing samples and performing pretreatment: performing type selection of equipment, and compounding a reagent and a culture medium; performing strain preservation, performing strain activation and preparing bacterium suspension; establishing a standard curve of OD value-viable bacterium content log lgC, and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of lgC-relative fluorescence intensity log lgI; performing sample inoculation, performing sample culture and performing eluting and recovering; determining I of recovered liquid, and reckoning the content C and T of the viable bacteria; calculating bacterium resisting rate R or bacterium resisting activity value A; and performing result evaluation. The method has the characteristic that the mold resisting properties of the leather, which are expressed by R or A, can be accurately and quantitatively tested through an ATP fluorophotometer. Control samples and bacterium resisting samples after contact for 0h and culturing for 48h are eluted and recovered according to the specification of the invention, the I of the recovered liquid of the control samples and the I of the recovered liquid of the bacterium resisting samples are determined and are expressed with lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the mold resisting properties of the leather, researched and developed by the invention, product quality increase can be supported through a scientific advanced detection technique.

Description

technical field [0001] The invention relates to a method for testing the mildew resistance of antibacterial leather, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the mildew resistance of antibacterial leather characterized by an antibacterial rate R or an antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of leather antibacterial function detection. Background technique [0002] Leather is a necessity of human life. Due to the introduction of a large amount of fatliquor in the process of tanning and the use of raw hides rich in protein, fat and other nutrients, it is extremely susceptible to microbial infection such as bacteria and fungi. With the improvement of people's living standards and health awareness, the requirements for antibacterial performance of leather products are constantly rising; antibacterial leather has emerged as the times require...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field aimed at the current development trend of quantification, rapidity and simplification, has drawn on the principle of ATP fluorescence analysis to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity oftextile products (textiles- Determination of Antibacterial Performance of Antibacterial Finished Textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorometric assay of anti-microbial / mold performance by content change, but it is only applicable to textile or microporous materials with water absorption and control sample / mycotic growth value > 0. It is not applicable to materials such as leather and plastics with non-absorbent hard surfaces and control sample mold growth value <0, and does not provide measurement uncertainty evaluation

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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