Method for detecting antifungal performance of coated antibacterial glass by ATP biological fluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting antifungal performance of antibacterial glass. The method comprises the following detection steps: sample preparation and pretreatment; equipment selection, and reagent and culture medium preparation; strain preservation and activation, and bacterial suspension preparation; counting of viable content CB blood cell plate of a bacterial suspension after the methylene blue staining; establishment of a standard curve of ATP concentration log value lgC-relative fluorescence intensity log value lgI, and calibration of living bacteria C of an inoculation bacterial liquid; sample inoculating and culturing, and eluting recovery; determination of recovery liquid I, and calculation of living bacteria ATP concentration C and T; calculation of antibacterial rate R or the antibacterial activity value A; and result evaluation. The method is characterized in that: an ATP fluorophotometer is used for accurately and quantitatively testing the anti-bacterial performance of ceramics characterized by R or A. The method comprises the following steps: eluting and recovering a control sample and an antibacterial sample after contacting for 0h and culturing for 24h / 48h, determining recovery liquid I, and characterizing and calculating R or A by the lgI; and providing a result evaluation basis. The ATP biological fluorescence lgC-lgI standard curve method for detecting the antifungal performance of glass developed by the invention can support the product quality improvement by using an advanced detection technology.

Description

technical field [0001] The invention relates to a method for testing the antifungal performance of antibacterial glass, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antifungal performance of antibacterial glass characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of glass antibacterial function detection. Background technique [0002] As a new type of ecological functional material, antibacterial glass adopts nano "dispersion" and "modification" technology to combine antibacterial agent with glass in a chemical bonding way, which belongs to the highest level of coated glass; it is used in medical care, food preservation, transportation and 3C electronic touch products and other fields are widely used. According to the global market observation report, the antibacterial glass market size in 2017 was 180 millio...

AI Technical Summary

Problems solved by technology

In view of the current development trend of quantification, rapidity and simplification in the research field of foreign antibacterial material performance testing technology, the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-antibacterial finishing textiles) Determination of antibacterial performance of textile products)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products.Part1 Luminescence (textiles - Determination of antifungal performance of antifungal finishing textiles)", which stipulates that the change of ATP content after sample inoculation is used to characterize Fluorescence measurement method for anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0. It is not applicable to glass, ceramics and other inorganic non-metallic materials with non-absorbent hard surface and control fungal growth value < 0; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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