Method for detecting antibacterial performance of antibacterial silicone rubber with ATP (adenosine triphosphate) bioluminescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting antibacterial performance of antibacterial silicone / rubber. The method comprises detection steps as follows: sample preparation and pretreatment; selection of equipment type and preparation of reagents and culture media; strain preservation and activation and preparation of a bacterial suspension; establishment of an ATP (adenosine triphosphate) concentration logarithm value lgCA-relative fluorescence intensity logarithm value lgIA standard curve and calibration of viable bacteria ATP concentration CA of an inoculant liquid; inoculation, culture and eluting recovery of samples; IA determination of a recovery liquid and calculation of viable bacteria ATP concentration CA and TA of the recovery liquid; calculation of antibacterial ratio R or antibacterial activity value A; result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurate and quantitative test of the antibacterial performance of R or A characterized silicone / rubber. According to the method, a control sample and an antibacterial sample after 0 h contact and 24 h culture are eluted and recovered, and IA of the recovery liquid is measured and R or A is characterized by lgIA and calculated; result evaluation basis is provided. According to the developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the antibacterial performance of antibacterial silicone / rubber, product quality improvement is supported with a scientific and advanced detection technology.

Description

technical field [0001] The present invention relates to the antibacterial performance testing method of antibacterial silicon / rubber materials and products, specifically a method for accurately quantitatively detecting the antibacterial performance of antibacterial silicon / rubber characterized by antibacterial rate R or antibacterial activity value A by using an ATP fluorescence photometer ATP bioluminescent lgC A -lgI A The standard curve method belongs to the technical field of silicon / rubber antibacterial function detection. Background technique [0002] Silicone / rubber products are widely used in the fields of industry and people's livelihood. Since the vulcanization accelerators, reinforcing fillers and other substances used in the processing process provide the necessary nutrient sources for the growth of bacteria, molds and other microorganisms, it is very easy to contaminate bacteria and cause its appearance and The physical and chemical properties are reduced; at ...

AI Technical Summary

Problems solved by technology

However, the existing detection methods have the following common problems in technical content and practical application: first, the number of experimental bacteria involved is too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; second, the experimental process is cumbersome and related operations Affected by the professional experience of the experimenter, the test error is large and lacks comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic cost are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of Textile products (textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content after sample inoculation is a fluorometric method to characterize the anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample bacteria / mold growth value > 0. In the recovery mode of live bacteria, test Key technical aspects such as effective conditions do not apply to materials such as plastics, silicon / rubber, etc., which have non-absorbent hard surfaces and control bacterial growth values ​​< 0; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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