Method for detecting anti-mildew property of antibacterial wood board by ATP bio-fluorescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting anti-mildew property of an antibacterial wood board, and the detecting steps include: sample preparation and pre-treatment; equipment selection, and reagent and medium preparation; species preservation and activation, and preparation of spore liquid; establishment of OD value-live bacteria content logarithmic value lgCB standard curve; establishmentof ATP concentration logarithmic value lgCA-relative fluorescence intensity logarithm lgIA standard curve and inoculated bacterial liquid live bacteria CA calibration; sample inoculation, culture andelution recovery; IA determination of recovered liquid and its live bacteria ATP concentration CA and TA reckoning; antibacterial rate R or antibacterial activity value A calculation; and results evaluation. The method is characterized in that an ATP fluorescence photometer is used to accurately and quantitatively test the mildew resistance of the wood board characterized by R or A. The method prescribes the elution and recovery of a control sample and an antibacterial sample after contact for 0 h and culture for 48 h, and the recovered liquid IA is determined and R or A is characterized and calculated by lgIA. The ATP bio-fluorescence lgCA-lgIA standard curve method supports the product quality improvement by a scientific and advanced detection technology.

Description

technical field [0001] The invention relates to a method for testing the mildew resistance of antibacterial wooden boards, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the mildew resistance of antibacterial wooden boards characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of antibacterial performance detection of wooden boards. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, the indoor environment and the quality of home decoration have attracted much attention. A new generation of wood-based panel products with anti-mildew and antibacterial properties has emerged at the historic moment and has a broad market prospect; it can be seen that antibacterial technology is widely used in the wood panel industry. The application and popularity of a...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field aimed at the current development trend of quantification, rapidity and simplification, has drawn on the principle of ATP fluorescence analysis to formulate ISO20743:2007-2013 "Textiles-Determination of antibacterial activity oftextile products (textiles-antibacterial activity) Determination of antibacterial performance of finished textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorescence determination of bacteria / mold properties, but it is only applicable to textile or microporous materials with water absorption and control sample bacteria / mold growth value > 0, and is not suitable for key technologies such as the recovery method of viable bacteria and the effective conditions of the test. Wooden boards, plastics and other materials with non-absorbent hard surface and control mold growth value < 0, and no measurement uncertainty evaluation is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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