Method for detecting mold resistance of antibacterial plastic by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a detection method for mold resistance of antibacterial plastic. The detection method comprises the detection steps of preparing and preprocessing samples; performing equipment selection and preparing a reagent and a culture medium; preserving and activating strains, and preparing a bacterium suspension; establishing a standard curve of OD value-viable bacteria content log lgC , and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of lgC-relative fluorescence intensity log lgI ; performing sample inoculating, culturing, eluting and recovering; performing I determination of the recovered liquid, and reckoning the viable bacteria content C and T; calculating antibacterial rate R or antibacterial activity value A; and performing result evaluation. The method has the characteristics that the mold resistance, expressed by R or A, of the plastic is subjected to pinpoint quantitative test through an ATP fluorophotometer. Control samples and antibacterial samples after contact for 0h and culture for 48h are eluted and recovered according to the specification of the invention, the I of recovered liquid is measured and expressed with lgI, and R or A is calculated. A result assessment basis is provided. The ATP biofluorescence lgC-lgI standard curve method for detecting mold resistance of the antibacterial plastic, which is researched and developed by the invention, supports improvement of product quality by a scientific and advanced detection technique.

Description

technical field [0001] The invention relates to a method for testing the anti-mold performance of antibacterial plastics, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the anti-mold performance of antibacterial plastics characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of plastic antibacterial function detection. Background technique [0002] Plastic products are widely used in the fields of industry and people's livelihood. Because the additives used in the processing process provide the necessary nutrient source for the growth of bacteria, molds and other microorganisms, the sterility of plastics and their surfaces is very important for reducing the spread of diseases and improving people's health. level is very important. Since the 1990s, developed countries represented by Japan and the United States have b...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-determination of antibacterial activity - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorometric assay of anti-microbial / mold performance by content change, but it is only applicable to textile or microporous materials with water absorption and control sample / mycotic growth value > 0. Aspects are not applicable to plastics, glass and other materials with non-absorbent hard surfaces and control sample mold growth value <0, and no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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