atp bioluminescent lgc b -lgi b Method for evaluating fungicidal effect of liquid disinfectant by standard curve method

Abstract

The invention relates to a method for evaluating the fungus killing effect of a liquid disinfectant. The evaluation steps include: sample extraction and determination of the minimum effective concentration; The live bacteria content of bacterial suspension after staining C B Blood count and inoculum C B Calibration; lgC B ‑ Relative fluorescence intensity log value lgI B Standard song establishment; neutralizer identification and quantitative suspension test; recovery solution I B Determination and its live bacteria content C B and T B Calculation; Calculation of killing rate R and sterilization index A; Judgment of results; The special feature is: the use of ATP fluorescence photometer to accurately and quantitatively evaluate the fungal killing effect of disinfectants characterized by R or A. The present invention stipulates to reclaim the test sample liquid and the control sample liquid after the different time of action, measure its I B and with lgI B Characterize and calculate R or A; provide the basis for judging the results. ATP bioluminescent IgC for the evaluation of the fungal killing effect of the disinfectant developed by the present invention B ‑lgI B The standard music method can improve the relevant evaluation method system of disinfection effect.

Description

technical field [0001] The invention relates to a method for evaluating the fungicidal effect of a liquid disinfectant, in particular to an ATP method for accurately quantitatively testing the fungicidal effect of a liquid disinfectant characterized by a killing rate R or a sterilization index A using an ATP fluorescence photometer. bioluminescent lgC B -lgI B The standard curve method belongs to the technical field of disinfectant sterilization effect evaluation. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, in order to cut off the transmission of infectious diseases, liquid disinfectants such as peroxides, aldehydes, phenols, and quaternary ammonium salts are widely used in medical treatment, food, education, catering, and home use. The field of application is becoming more and more extensive, and the killing effect and broad-spectrum requirements for pathogenic microorganisms such as bacteria, fu...

AI Technical Summary

Problems solved by technology

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-determination of antibacterial activity - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorometric assays to characterize anti-bacteria / mold performance by content change, but the absorption method, transfer method and transfer method provided are only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0; The evaluation of the fungicidal effect of liquid disinfectants must form a set of clear and specific methods for key technical aspects such as neutralization effect evaluation and result calculation formula, and also provide relevant measurement uncertainty evaluation

In addition, the antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the traditional killing rate R in my country is

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