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43 results about "Blood count" patented technology

Method and System for Analyzing a Blood Sample

Methods, systems, and computer program products for the analysis of a blood sample are disclosed. One embodiment is a method of detecting and enumerating hard-to-ghost cells in a blood sample. Another embodiments is a method of analyzing reticulocytes in a blood sample. Methods of using blood count parameters are also provided.
Owner:BECKMAN COULTER INC

Performance improvement for hematology analysis

A method for determining the value of MCV of a fresh sample of blood when the value of MCV for that sample of blood is known, but the period of time that the sample of blood has been stored is not known. The method of this invention allows an automated hematology analyzer to provide a more reliable indication of the initial value of MCV of a sample of blood, i.e., the value of MCV that would have been expected for the sample of blood when the sample was fresh. Furthermore, the method of this invention uses data that is readily available as part of the blood count data.
Owner:ABBOTT LAB INC

Determination method of quantity of cassava pollen

InactiveCN110132822ASolve the unstable state of samplingIncrease concentrationIndividual particle analysisSODIUM METAPHOSPHATESucrose
The invention discloses a determination method of the quantity of cassava pollen. The method includes steps: taking and putting 10 fully mature, plump and uncracked cassava anthers into a 2 ml centrifugal tube, and placing the centrifugal tube in a dryer in an incubator at the temperature of 30 DEG C for accelerating cracking; after completion of spilling of pollen, adding dispersing mixing liquid, and performing uniform oscillation on a vortex oscillator to form suspended pollen liquid, wherein the dispersing suspended mixing liquid is formed by 20% of sodium metaphosphate, 2% of sucrose, 2%of glucose, 0.01% of hydroxypropyl methyl cellulose, 0.04%-0.06% of xanthan gum, and the remaining water; and taking and dripping the suspended pollen liquid on a slide, and performing observation andstatistics on the particle quantity of pollen under a microscope. According to the method, the condition that the particle size of the cassava pollen is greater than 0.10 mm so that the counting cannot be performed by employing a blood counting chamber determination method for determination of the pollen quantity is overcome; besides, through selection of proper dispersing mixing liquid, the stability of the pollen suspension liquid is improved, the deposition speed of pollen is controlled, and observation is easier and counting is more accurate during determination by adopting a microscope observation method.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI

Culture method of trichoderma producing cellulase

The invention discloses a culture method of trichoderma producing cellulase. The method includes: inoculating the trichoderma onto a PDA slant, culturing at 45 DEG C for 5 d, washing spores with 5 mL of sterilized normal saline after the spores are formed, counting the collected spore solution by using a blood counting chamber so as to allow the number of the spores in each milliliter of the solution to be 106-107, inoculating onto a solid fermentation culture medium according to a 5% inoculating amount, and culturing at 28 DEG C for 146 h. The enzyme output in unit volume in trichoderma solid-state fermentation is higher than that of liquid fermentation. The method has characteristics of no stirring, low energy consumption, low equipment investment, simple post treatment and basically no pollution.
Owner:QINGDAO ZHONGREN PHARMA

Cell wall broken Ganoderma Lucidum spore powder-Perilla herb oil composite softgel and preparation technology thereof

The invention discloses a cell wall broken Ganoderma Lucidum spore powder-Perilla herb oil composite softgel and a preparation technology thereof. The preparation technology comprises the following steps: 1, sieving Ganoderma Lucidum spore powder by a 20 mesh sieve, elutriating, separating, and drying at 60DEG C to obtain dried Ganoderma Lucidum spore powder for later use; 2, carrying out wall breaking of the dried Ganoderma Lucidum spore powder by adopting a roller type wall breaker, stopping the wall breaking when the wall breaking rate detected by a blood counting chamber reaches 90-97%, and carrying out sealing preservation of the cell wall broken Ganoderma Lucidum spore powder in 0-5DEG C environment to obtain cell wall broken Ganoderma Lucidum spore powder; 3, mixing the cell wall broken Ganoderma Lucidum spore powder with an oil agent composed of Perilla herb oil and vitamin E, carrying out mechanical stirring beating, carrying out colloid mill treatment to obtain a mixed slurry, and carrying out heat insulation at 50DEG C; and 4, adopting gelatin to prepare a capsule membrane, and pelletizing by using a pelletizing machine to obtain the cell wall broken Ganoderma Lucidum spore powder-Perilla herb oil composite softgel. A low temperature wall breaking process and the insulation of the softgel gelatin membrane to oxygen effectively protect the health components of the Ganoderma Lucidum spore powder and the Perilla herb oil.
Owner:FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI

Method for increasing embryoid emergence quantity in brassica chinensis isolated microspore culture

The invention provides a method for increasing embryoid emergence quantity in brassica chinensis isolated microspore culture. The method includes: step one, selecting a brassica chinensis bud in a late mononuclear stage, disinfecting, separating and purifying to obtain isolated microspores, suspending the purified microspores in an NLN liquid culture medium, and adjusting the microspore density to1*10<5>-2*10<5> microspores/mL by a blood counting chamber to obtain microspore suspension; step two, adding the prepared brassica chinensis microspores into a NLN-13 culture medium in which macroelements are reduced by half and 6-BA (0.05mg/L), NAA (0.10mg/L) and activated carbon (100mg/L) are added, subjecting sub-packaged microspore petri dishes to 32 DEG C heat shock treatment in a constant-temperature incubator for 24h, and then transferring into a 25 DEG C constant-temperature incubator for continuing dark culture until embryoids appear. By adoption of the method, an evident promoting effect on brassica chinensis microspore induced embryoid emergence is achieved, the microspore embryoid emergence quantity can be increased. The method has advantages of practicality, specific steps, high repeatability and the like, and extensive application of brassica chinensis isolated microspore culture to haploid breeding can be realized.
Owner:海南省农业科学院蔬菜研究所

Blood component separation device

This blood component separation device: is provided with a centrifugal separator for separating a plurality of prescribed blood components from blood, and a container for holding the prescribed blood components that have been centrifugally separated; and performs a step in which the separated plurality of blood components are each collected over a plurality of cycles. The blood component separation device performs a step in which the starting point of the step for collecting the prescribed blood components is determined from blood count values of a donor on the basis of map data pertaining to pre-stored blood count values or the concentrations of prescribed blood components, in such a manner that the concentrations of the prescribed blood components become equal at each point that the blood components are discharged from the centrifugal separator at the collection starting point and finishing point in the step for collecting the prescribed blood components.
Owner:TERUMO KK

Countable drainage bag

The invention relates to the technical field of blood volume measuring equipment, in particular a countable drainage bag, comprising a blood drainage bag, wherein the blood drainage bag includes a bagbody, a blood counting calibration area is arranged on the outer surface of the bag body, a drainage structure is arranged at the center of the bag body, a drainage structure includes a drainage tube, the top of the drainage tube is connected with a finite flow tube, the diameter of the current limiting tube from top to bottom increases from small to large, an end portion of the flow restrictiontube remote from the drainage tube passes through the top surface of the bag body and extends to the outside of the bag body, one end part of the flow restriction tube away from the flow restriction tube is connected with a blood drawing connecting tube, the outer wall of the flow restriction tube is provided with uniformly equidistant arranged through holes, one end part of the flow restriction tube away from the flow restriction tube is connected with a sealable tube, a mesh cover is installed at the bottom of the flow restriction tube, and a floating ball is placed inside the flow restriction tube. The invention can read out the blood volume in the bag through the blood counting calibration area arranged on the bag body, and the recording panel can assist the medical personnel to recordthe blood volume.
Owner:THE SECOND AFFILIATED HOSPITAL OF NANJING MEDICAL UNIV

Method for performing experiment for observing molecular motion

The invention relates to a method for performing an experiment for observing molecular motion, which comprises the following steps: grinding, during which water is filled in a vessel and grinding an ink stick in the vessel; liquid taking, during which 1 to 3 drops of liquid is taken by a burette after the ink stick is ground in the vessel for a plurality of times and placed into a groove on a blood counting chamber, cover glass is covered, and excessive water on the edge is absorbed by paper; and the placement of the blood counting chamber on a viewing platform of a microscope and the adjustment of the microscope for observation of 'molecular motion trace' represented by a plurality of carbon particles from an ocular lens. A camera on a video display stand can be aimed at the ocular lens of the microscope vertically, and the focus of the video display stand is adjusted till 'molecular random motion' represented by a plurality of carbon particles can be seen on the large screen. According to the technical proposal, the 'molecular motion trace' can be observed with a low power biological microscope in a common middle school by using ink produced by grinding an ink stick in the vessel, single-student observation can be changed into the real-time common observation of the students in class by the conversion and amplification of the video display stand, and a teacher can give real-time analysis and explanation. Thus, the experiment is more visual and universal, the interests of students are aroused, and the classroom teaching effect is improved.
Owner:赵绍兴

Method for quickly finding early bacterium infection in microorganism fermentation process

PendingCN110923290ALow priceEarly detection of bacterial infectionMicrobiological testing/measurementBiotechnologyMicroorganism
The invention relates to a method for finding early bacterium infection of fermentation liquor. The method comprises the following steps of preparing culture mediums, performing inoculation into a strain to be fermented, and beginning fermentation; performing fermentation for some time, and before the fermentation liquor reaches a given OD value, taking out fermentation liquor samples; calculatingthe bacterium concentration of the taken-out fermentation liquor samples with a blood cell counting chamber, and according to the bacterium concentration of the fermentation liquor samples, determining the multiple to be concentrated of the fermentation liquor samples, wherein the concentration multiple is large enough to concentrate the bacterium concentration of the sampled fermentation liquorsamples to the concentration of given microscope inspection bacteria which are suitable for observation under a microscope; according to the determined concentration multiple, concentrating the fermentation liquor samples to the concentration of the microscope inspection bacteria; observing the concentrated samples having given area under the microscope, and determining whether infectious microbesexist or not and the number of the infectious microbes; and based on the existence and the number of the infectious microbes contained in the concentrated samples having the given area, judging wither the fermentation liquor has a phenomenon of bacterium infection or not.
Owner:BLOOMAGE BIOTECHNOLOGY CORP LTD

atp bioluminescent lgc b -lgi b Method for evaluating fungicidal effect of liquid disinfectant by standard curve method

The invention relates to a method for evaluating the fungus killing effect of a liquid disinfectant. The evaluation steps include: sample extraction and determination of the minimum effective concentration; The live bacteria content of bacterial suspension after staining C B Blood count and inoculum C B Calibration; lgC B ‑ Relative fluorescence intensity log value lgI B Standard song establishment; neutralizer identification and quantitative suspension test; recovery solution I B Determination and its live bacteria content C B and T B Calculation; Calculation of killing rate R and sterilization index A; Judgment of results; The special feature is: the use of ATP fluorescence photometer to accurately and quantitatively evaluate the fungal killing effect of disinfectants characterized by R or A. The present invention stipulates to reclaim the test sample liquid and the control sample liquid after the different time of action, measure its I B and with lgI B Characterize and calculate R or A; provide the basis for judging the results. ATP bioluminescent IgC for the evaluation of the fungal killing effect of the disinfectant developed by the present invention B ‑lgI B The standard music method can improve the relevant evaluation method system of disinfection effect.
Owner:李文杰

Method for separating mononuclear cells from whole blood collection device for blood donation

The invention discloses a method for separating peripheral blood mononuclear cells from a repayment-free blood donation whole blood collection device, which comprises the following steps: clamping two side tube walls by using a tube clamp under a sterile condition, cutting open upper and lower end tube orifices of the two side tube walls by using sterile scissors, and reserving a section of long tube on each of the two side tube walls; using an injector to forward flush the white filter and backward flush the white filter with the flushing fluid, collecting the liquid obtained by backward flushing, centrifuging, sucking and discarding the supernatant after centrifuging, re-suspending cells with normal saline, adding into another two centrifugal tubes filled with the same amount of Ficoll, and continuing centrifuging; and after centrifugation is finished, sucking and abandoning supernatant, sucking the albuginea layer above the Ficoll into a new centrifuge tube, supplementing normal saline, sucking and abandoning supernatant after centrifugation, and manually counting by adopting a blood cell counting plate after the normal saline is resuspended. According to the method disclosed by the invention, the mononuclear cells can be aseptically extracted from the white filter of a blood donation and collection pipeline and cultured in vitro, and the cells cultured in vitro are used as DC-CIK treatment cells.
Owner:QINGDAO BLOOD CENT
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