Method for evaluating fungus destroying effect of daily chemical products by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for evaluating the fungus destroying effects of daily chemical products. The method comprises the detection steps of meeting requirements of sample extraction and pretreatment; performing type selection of equipment, and compounding a reagent and a culture medium; performing strain preservation, performing strain activation and preparing fungus suspension; after methylthioninium chloride staining, counting the content C of viable fungi in the fungus suspension through a hemocytometer; establishing a standard curve of ATP concentration log lgC-relative fluorescence intensity log lgI, and performing calibration on C of viable fungi in inoculated fungus liquid; performing neutralizer identification and performing fungus destroying experiment; determining I of recovered liquid, and reckoning C and T of the recovered liquid; calculating fungus destroying rate R and fungus destroying index A; and performing result evaluation. The method has the characteristic that the fungus destroying effects of the daily chemical products, which are expressed by R or A, can be accurately and quantitatively evaluated through an ATP fluorophotometer. Control samples and experiment samples after special time of fungus infection are recovered according to the specification of the invention, the I of the recovered liquid of the control samples and the I of the recovered liquid of the experiment samples are determined and expressed by lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the fungus destroying properties of the daily chemical products, researched and developed by the invention, product quality increase is supported through an advanced detection technique.

Description

technical field [0001] The invention relates to a method for evaluating the fungus killing effect of daily chemicals, in particular to an ATP bioluminescent method for precise and quantitative testing of the fungus killing effect of daily chemicals represented by the sterilization rate R or the sterilization index A by using an ATP fluorescence photometer lgC A -lgI A The standard curve method belongs to the technical field of bactericidal effect evaluation of daily chemicals. Background technique [0002] In recent years, with the improvement of people's living standards and changes in consumption concepts, the domestic market's demand for daily chemical products such as washing and care has been increasing year by year; according to the "2018-2023 Development Prospects of China's FMCG Industry" And Investment Opportunity Analysis Report" shows that China's daily chemical consumption market as a whole is showing a continuous growth trend higher than the GDP growth rate. ...

AI Technical Summary

Problems solved by technology

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles- Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity oftextile products. Fluorescence assays to characterize anti-bacteria / mold performance, but the absorption method, transfer method and transfer method provided by it are only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0; The evaluation of the fungicidal effect of chemicals must form a set of clear and specific methods for key technical aspects such as neutralization effect evaluation and result calculation formulas, and at the same time provide relevant measurement uncertainty evaluation

In addition, the antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the traditional killing rate R in my country is

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