The invention provides application of Gracilariopsis lemaneiformis polysaccharide and an antitumor drug containing Gracilariopsis lemaneiformis polysaccharide. The application refers to application of the Gracilariopsis lemaneiformis polysaccharide as a synergist in antitumor drug preparation. The antitumor drug contains a Gracilariopsis lemaneiformis polysaccharide synergist, antitumor active components and pharmaceutically acceptable excipients. The invention for the first time finds that combined use of seaweed Gracilariopsis lemaneiformis polysaccharide with chemotherapy drugs paclitaxel, fluorouracil, methotrexate, cisplatin and the like can increase the treatment effect of chemotherapy drugs on tumors. The combined use of Gracilariopsis lemaneiformis polysaccharide with chemotherapy drugs can achieve the effect of inhibiting tumor proliferation and activity on a plurality of tumor cells with different tissue origins, including lung cancer, gastric cancer, cervical cancer and the like, the synergistic effect of Gracilariopsis lemaneiformis polysaccharide on chemotherapy drugs is dose-dependent, and in Gracilariopsis lemaneiformis polysaccharide in-vitro cell experiments, the working concentration does not show toxicity, with the characteristics of rich medicine sources, safety and no toxicity, the Gracilariopsis lemaneiformis polysaccharide can be developed into antitumor drugs or preventive health care products used for antitumor chemotherapy drug synergists, and has clinical application prospect.