Method for evaluating fungi destroying effects of disposable sanitary accessories by ATP fluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for evaluating the fungus destroying effects of disposable sanitary accessories. The method comprises the detection steps of determining requirements of sample extraction and the like; performing type selection of equipment, and compounding a reagent and culture mediums; performing strain preservation, performing strain activation and preparing bacterium suspension; counting the viable bacteria content C of bacterium suspension after methylene blue dyeing with a hemocytometer, and performing calibration on C of inoculation bacterial liquid; establishing a standard curve of the lgC-relative fluorescence intensity logarithm value lgI ; performing neutralizer identification and performing bacterium destroying experiment; determining I of recovery liquid, and reckoning viable bacteria content C and viable bacteria content T; calculating bacterium destroying rate R and bacterium killing index A; and performing result evaluation. The method has the characteristic that the fungus destroying effects which are represented by R or A, of the sanitary accessories can be accurately and quantitatively evaluated through an ATP fluorophotometer. Control samples and experiment samples after fungus infection for different time are eluted and recovered according to the specification of the invention, the I of the recovery liquid is determined and represented by lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the fungus destroying properties of the disposable stainless steel, researched and developed by the invention, product quality increase is supported through an advanced detection technique.

Description

technical field [0001] The invention relates to a method for evaluating the fungus killing effect of disposable sanitary products, in particular to an ATP method for precise and quantitative testing of the fungal killing effect of disposable sanitary products characterized by a sterilization rate R or a sterilization index A using an ATP fluorescence photometer bioluminescent lgC B -lgI B The standard curve method belongs to the technical field of bactericidal effect evaluation of disposable sanitary products. Background technique [0002] In recent years, with the acceleration of urbanization and changes in consumer concepts, the demand for disposable sanitary products in the domestic market has increased year by year; in 2017, the total market sales were close to 80 billion yuan, with a compound annual growth rate of 13.3% and exports It is larger than imports; according to the forecast of authoritative organizations, the global disposable sanitary products market will e...

AI Technical Summary

Problems solved by technology

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-determination of antibacterial activity - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorescence determination method of content change to characterize anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample bacteria / mold growth value > 0; the evaluation of the bacteria-killing effect of disposable hygiene products must be based on Form a set of clear and specific methods for key technical aspects such as neutralization effect evaluation and result calculation formula, and provide related measurement uncertainty evaluation

In addition, the antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the traditional killing rate R in my country is

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

View more