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Nano carrier material as well as preparation method and application thereof

A technology of nano-carriers and lipoic acid, applied in the field of medicine, can solve the problems of non-specific targeted drug delivery of chemotherapy, enhanced toxic reactions, and increased economic burden, and achieve the effects of stable properties, low toxicity, and low cost

Inactive Publication Date: 2017-05-31
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional chemotherapy cannot specifically target drug delivery, which causes normal healthy cells to be attacked and damaged by drugs, which limits the maximum allowable dose. What's more, the rapid elimination and non-specific distribution of target organs and tissues make patients often need to increase the dose. Large doses lead to increased economic burden and enhanced toxicity

Method used

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  • Nano carrier material as well as preparation method and application thereof
  • Nano carrier material as well as preparation method and application thereof
  • Nano carrier material as well as preparation method and application thereof

Examples

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preparation example Construction

[0039] The preparation method of the biodegradable nano-carrier material and drug-loaded nanoparticles of the present invention, the specific steps include:

[0040] (1) Preparation of materials: lipoic acid was dissolved in methanol, and cysteine ​​hydrochloride was added to react for 1 to 24 hours;

[0041] (2) Washing to remove impurities: After removing excess methanol, wash with dilute hydrochloric acid for 2-3 times, and wash with water for 3 times to further remove impurities

[0042] quality;

[0043] (3) Preparation of nanoparticles: dissolve in chloroform, ultrasonically emulsify 1% sodium cholate solution to obtain nanoparticles, and disperse them in distilled water;

[0044] (4) Centrifuge to remove large particles (3000r, 10min);

[0045] (5) Purification: remove sodium cholate by ultrafiltration (100000 molecular weight, 1500r, 3min);

[0046] (6) Drug loading: the solution obtained in step (4) was lyophilized and dissolved in chloroform, added antineoplastic ...

Embodiment 1

[0048] Example 1 Synthesis of blank nanoparticles (i.e. nanocarrier material) and drug-loaded nanoparticles

[0049] (1) Dissolve 100mg of lipoic acid in 1ml of methanol, add 170mg of cysteine ​​hydrochloride, and stir in the dark for 8 hours. The reaction structure is shown in figure 1 .

[0050] (2) Air-dry or dry methanol with nitrogen, wash the solution obtained in step (1) 2-3 times with dilute hydrochloric acid to remove unreacted cysteine ​​hydrochloride, and then wash with distilled water 3 times 3 times, after removing excess hydrochloric acid dry.

[0051] (3) Dissolve the material obtained in step (2) in 2ml of chloroform, then add 8ml of 1% sodium cholate, and ultrasonicate for 30s with an interval of 10s. Repeat the sonication 2 times.

[0052] (4) Add the solution obtained in step (3) into 10 ml of distilled water, stir for 12 hours, and evaporate the chloroform.

[0053] (5) Centrifuge (3000r, 10 minutes) the nanoparticles obtained in step (4) to remove larg...

Embodiment 2

[0058]Example 2 Lipoic Acid Nanoparticle Preparation Prescription Screening

[0059] In the lipoic acid methanol solution, add 10%, 20%, 30%, 40%, 50% cysteine ​​respectively, measure the 330nm place ultraviolet absorption value of the carrier material of different reaction times after reacting for 24 hours, the particle size of nanoparticle As a result, when adding 20% ​​cysteine, the characteristic ultraviolet absorption value is the lowest, the crosslinking is the most complete, and the particle size is the lowest; lipoic acid reacts with 20% cysteine ​​1, 2, 4, 8, 12, After 24 hours, measure the UV absorption value at 330nm of the carrier material and the particle size of the nanoparticles. As a result, after 8 hours, the characteristic UV absorption value is the lowest, the crosslinking is the most complete, and the particle size is the lowest, and the prepared nanoparticles are placed at 4°C. After being stored for one month, the nanoparticles for 8 hours, 12 hours, and ...

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Abstract

The invention discloses a nano carrier material which is a polymer formed by crosslinking of thioctic acid reduced and cracked by cysteine. The invention further discloses a preparation method and an application of the nano carrier material. The nano carrier material is degradable and low in cytotoxicity, has the advantage of tumor cell environment response, can realize targeted drug delivery for a tumor cell, improves the efficacy of an anti-tumor drug, reduces the pain of a patient, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a nano-carrier material and its preparation method and application. Background technique [0002] Tumors are characterized by abnormal cell growth at an uncontrolled rate, and the disease remains the second leading cause of death in the world. The current methods of treating tumors include surgery, radiotherapy, hormone therapy and chemotherapy, among which chemotherapy is the main method. Traditional chemotherapy cannot specifically target drug delivery, which causes normal healthy cells to be attacked and damaged by drugs, which limits the maximum allowable dose. What's more, the rapid elimination and non-specific distribution of target organs and tissues make patients often need to increase the dose. Large doses lead to increased economic burden and enhanced toxicity. Therefore, providing a targeted drug delivery system with low toxicity is the key to solving the defects of...

Claims

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Application Information

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IPC IPC(8): A61K47/34A61P35/00C08G75/02
Inventor 高申顾芬芬胡楚玲姚翀高原张丽娟田泾曹伟宫春爱夏清明
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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