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Preparation method and application of magnetic silicon dioxide composite microsphere

A technology of silicon dioxide and composite microspheres, applied in the field of immunochemical tests, can solve the problems of poor compression and corrosion resistance of microspheres, easy corrosion of magnetic core ferric oxide, etc. The effect of good dispersion and simple purification steps

Active Publication Date: 2014-07-02
HARBIN YICAI NEW MATERIAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent 200410057258.1 applied for a biopolysaccharide polymer magnetic microsphere and its preparation method, and Chinese patent 200510116850.9 applied for a preparation method of a composite biopolysaccharide magnetic microsphere. The microspheres prepared by the above method have poor compression and corrosion resistance ; Chinese patent 200610147250.3 applied for nano-magnetic microspheres with amino groups immobilized on the surface of protease and its preparation method and application, and Chinese patent 200710047399.9 applied for the preparation method of magnetic microspheres with surface functional groups. Application after amination, the ferroferric oxide core is easily corroded

Method used

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  • Preparation method and application of magnetic silicon dioxide composite microsphere
  • Preparation method and application of magnetic silicon dioxide composite microsphere

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Experimental program
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Effect test

Embodiment 1

[0035] 1. Fe 3 o 4 Preparation of nanoparticles

[0036] 1. Preparation of iron oleate precursor

[0037] 2.7gFeCl 3 ·6H 2 O was dissolved in a mixed solution of water, ethanol and n-hexane with a volume of 70mL, and then 8.875g of sodium oleate was added, stirred magnetically under a nitrogen atmosphere at room temperature, heated to 70°C, condensed and refluxed for 4h. After the reaction was completed, the instrument was closed, and the solution was cooled to room temperature, separated by a separatory funnel, the upper organic phase contained a mixture of ferric oleate, the upper organic phase was washed with deionized water, dried in vacuo, and the solvent n-hexane was removed to obtain brown The brown waxy solid is the iron oleate precursor.

[0038]2. Preparation of Fe by high temperature pyrolysis of iron oleate precursor 3 o 4 Nanoparticles

[0039] Dissolve 1 g of iron oleate precursor in 5.56 mL of octadecene, add 0.16 mL of oleic acid, stir magnetically, he...

Embodiment 2

[0051] 1. Fe 3 o 4 Preparation of nanoparticles

[0052] 1. Preparation of iron oleate precursor

[0053] 2.7gFeCl 3 ·6H 2 O was dissolved in a mixed solution of water, ethanol and n-hexane, with a volume of 70 mL, and then 8.875 g of sodium oleate was added, stirred magnetically under a nitrogen atmosphere at room temperature, heated to 50 ° C, condensed and refluxed for 6 h. After the reaction was completed, the instrument was closed, and the solution was cooled to room temperature, separated by a separatory funnel, the upper organic phase contained a mixture of ferric oleate, the upper organic phase was washed with deionized water, dried in vacuo, and the solvent n-hexane was removed to obtain brown The brown waxy solid is the iron oleate precursor.

[0054] 2. Preparation of Fe by high temperature pyrolysis of iron oleate precursor 3 o 4 Nanoparticles

[0055] Dissolve 1 g of iron oleate precursor in 5 mL of octadecene, add 0.15 mL of oleic acid, stir magnetically...

Embodiment 3

[0063] 1. Fe 3 o 4 Preparation of nanoparticles

[0064] 1. Preparation of iron oleate precursor

[0065] 2.7gFeCl 3 ·6H 2 O was dissolved in a mixed solution of water, ethanol and n-hexane with a volume of 70mL, and then 8.875g of sodium oleate was added, stirred magnetically under a nitrogen atmosphere at room temperature, heated to 80°C, condensed and refluxed for 3h. After the reaction was completed, the instrument was closed, and the solution was cooled to room temperature, separated by a separatory funnel, the upper organic phase contained a mixture of ferric oleate, the upper organic phase was washed with deionized water, dried in vacuo, and the solvent n-hexane was removed to obtain brown The brown waxy solid is the iron oleate precursor.

[0066] 2. Preparation of Fe by high temperature pyrolysis of iron oleate precursor 3 o 4 Nanoparticles

[0067] Dissolve 1 g of iron oleate precursor in 6 mL of octadecene, add 0.18 mL of oleic acid, stir magnetically, heat...

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Abstract

The invention relates to a preparation method and application of a magnetic silicon dioxide composite microsphere. Superparamagnetic ferroferric oxide nano particles of which the diameter ranges from 4 nm to 30 nm are prepared through a high-temperature pyrolysis method, a silicon dioxide shell of which the thickness ranges from 5 nm to 20nm covers the outer surfaces of the magnetic ferroferric oxide nano particles through a reverse microemulsion method, amination modification is conducted on the silicon dioxide surface, glutaraldehyde is used as a crosslinking arm, ligand protein is connected into, and protein separation is conducted through specific binding of ligand protein and target protein. The prepared magnetic microsphere is small in particle diameter and good in monodispersity, the composite microsphere with amine is large in specific surface area, nucleophilic addition is utilized, after the crosslinking arm glutaraldehyde is connected into, multiple kinds of ligand protein can be connected into, and then multiple kinds of target protein can be separated. The method is suitable for rapid separation and application of protein in biological samples, and has wide application prospect and great application value in the biomedical field and other fields.

Description

technical field [0001] The invention belongs to the field of immunochemical experiments, and relates to a magnetic silica composite microsphere and its preparation method and application Background technique [0002] Since the 1990s, with the development of nanotechnology, magnetic materials have entered a new era of magnetic nanomaterials. Compared with bulk magnetic materials, the magnetic properties of magnetic nanomaterials have changed greatly, and they have very broad application prospects, and have become a research hotspot in the world. The application of magnetic particles in protein separation and drug targeting is also one of the hot research topics in the current biological field. Among many magnetic nanomaterials, superparamagnetic Fe3O4 nanoparticles are easily metabolized and decomposed in organisms, so Fe3O4 nanoparticles have potential applications in biomedicine. A common method for the synthesis of monodisperse Fe3O4 nanoparticles is the high-temperature...

Claims

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Application Information

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IPC IPC(8): H01F1/11H01F1/42B01J13/02C12Q1/00
Inventor 赵九蓬赵长杰
Owner HARBIN YICAI NEW MATERIAL
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