Micro bacteria cracking reactor based on surface acoustic waves and bacteria cracking method

A pyrolysis reactor, surface acoustic wave technology, applied in specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, stress-stimulated microbial growth method, etc., can solve inapplicable rapid analysis, sample volume Large size, long processing time, etc., to achieve the effect of low attenuation, small sample volume, and short rupture time

Active Publication Date: 2019-06-11
XI AN JIAOTONG UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods used for bacterial lysis mainly include lysozyme lysis, mechanical lysis, ultrasonic lysis, centrifugation, etc., but these methods have large sample volume and long processing time, and are not suitable for rapid on-site analysis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro bacteria cracking reactor based on surface acoustic waves and bacteria cracking method
  • Micro bacteria cracking reactor based on surface acoustic waves and bacteria cracking method
  • Micro bacteria cracking reactor based on surface acoustic waves and bacteria cracking method

Examples

Experimental program
Comparison scheme
Effect test

example

[0037]Connect the injection hole 7 and the product outflow hole 9 at the upper cover plate 2 of the miniature bacterial lysis reactor based on the surface acoustic wave to the syringe with the bacterial suspension to be cracked on the syringe pump and the catheter for the bacterial lysate collection tube respectively. connected. Set the flow rate of the syringe pump to 1 mL / h. Input a pulse signal with a duty cycle of 33% and a frequency of 333Hz into the pulse signal generator. Connect the positive and negative poles of the output signal of the power amplifier to the interdigitated electrodes on the surface acoustic wave generating structure 3 respectively, adjust the output signal frequency of the pulse signal generator to be 13MHz, and the power input to the surface acoustic wave generating structure 3 is 14W; Turn on the signal control system and the syringe pump to lyse the bacteria. Escherichia coli, a commonly used experimental bacterium, was selected. Driven by the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a micro bacteria cracking reactor based on surface acoustic waves and a bacteria cracking method. The reactor comprises a lower bottom plate and an upper cover plate which arealigned and bonded together; a group of surface acoustic wave generation structures are arranged on the lower bottom plate; a groove is formed in one side of the upper cover plate, and a micro-channelstructure allowing liquid to flow through is arranged in the middle of the upper cover plate; a signal control system is used for controlling the surface acoustic wave generation structures; the micro-channel structure comprises a sample introduction hole, a reaction area and a product outflow hole. The method is suitable for cracking different types of bacteria, does not need any chemical reagent, and avoids the pollution of the chemical reagent to a cracked product.

Description

technical field [0001] The invention belongs to the technical field of micro-nano manufacturing application, relates to a detection device of a biochemical sample, in particular to a surface acoustic wave-based miniature bacterial lysis reactor and a bacterial lysis method. Background technique [0002] The study of bacteria at the molecular level is of great significance in disease pathology and drug screening. When analyzing bacteria, the bottleneck problem is how to lyse bacteria quickly and efficiently. The current methods for bacterial lysis mainly include lysozyme lysis, mechanical lysis, ultrasonic lysis, centrifugation, etc., but these methods have large sample volume and long processing time, and are not suitable for rapid on-site analysis. Contents of the invention [0003] The purpose of the present invention is to provide a micro-bacteria cracking reactor based on surface acoustic waves and a bacterial cracking method, which utilizes the acoustic radiation for...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M1/42C12M1/36C12N1/06
Inventor 郑腾飞芦海伟胡家栋周文陈家欢王朝晖
Owner XI AN JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap