Breeding method of humi grass using low energy heavy ion radiation mutagenesis

A technology for mutation breeding and Spartina vulgaris, applied in the biological field, can solve the problems of waste of reagents, complicated operation of culture technology, etc., and achieve the effects of saving reagents, saving labor, and reducing impact.

Inactive Publication Date: 2003-09-17
NANJING UNIV
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Problems solved by technology

However, none of the work involved the genetic improvement of Spartina foxii and the screening of

Method used

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Examples

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[0027] Four. Specific embodiments Example 1. Get 100 Spartina fox seeds, germinate after excision of non-embryonic ends, and obtain 61 seedlings altogether. Take the stem base of sterile seedlings and induce them on the induction medium containing MS basic element 4.6g / L, sucrose 30g / L, 2,4-D2.0mg / L, IAA1.0mg / L and Phytagel agarose 2g / L. callus, and 4 parts of green embryogenic tissue were isolated from it, and put into it containing 4.6g / L of MS basic elements, 30g / L of sucrose, 1.0mg / L of NAA, 0.5mg / L of 2,4-D, and 0.5mg of BA / L and Phytagel agarose 2g / L subculture medium. After multiplying to a certain number, 20 pieces of tissue were selected and immersed in 2% DMSO solution for 45 minutes. 15 ions cm -2 N + radiation. After 3 months of recovery, 6 tissues survived. Transfer it to the bud differentiation medium containing MS basic elements 4.6g / L, sucrose 30g / L, BA3mg / L and Phytagel agarose 2g / L to induce buds, then cut the buds, and transfer them to MS basic element...

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Abstract

A mutagenic method with low-energy heavy ion radiation for culturing the seeds of Humi herb used to build the grazing land in shoal and nunja includes such steps as low-energy heavy ion radiation to the embryonic calli of Humi herb to lead and mutation of genetic material, differentiating to generate regenerated seedlings, growing in artificial greenhouse, screening excellent line by testing its characteristics including protein content, and comparing DNA variation to obtain a new variety.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for selecting and breeding high-quality new strains of Spartina viticum by utilizing low-energy heavy ion radiation mutagenesis. 2. Background technology [0002] Spartina patens is a high-quality salt-tolerant forage grass, and it is also a common tool species for salinity restoration. There are a large number of bare tidal flats in the eastern coastal areas of my country, which lack suitable vegetation. The introduction and development of high-quality new strains of Spartina fox in this type of tidal flats is of great significance to the development of saline soil agriculture and restoration of tidal flats in my country. [0003] The introduction of Spartina fox in China began in the 1980s. There is a single domestic species, and there is no report on its genetic breeding. In the past, the reports of ion beam radiation mutation breeding were all focused on crop seeds an...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 钦佩周长芳朱晓佳
Owner NANJING UNIV
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