DNA (deoxyribonucleic acid) in-vivo sampling method for young Hyriopsis cumingii

A technology of triangular sailfish and live body, applied in the field of molecular biology, can solve the problems of loss of good individuals, no practical significance, difficult operation of DNA extraction, etc., and achieve the effect of shortening the research cycle, small size and good quality

Active Publication Date: 2012-04-25
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0003] Extracting a certain quality of high-concentration genomic DNA is the basis and premise of molecular biology research. It is relatively simple to extract DNA from living adult Hypophyllus sinensis, but it is very difficult to extract DNA from living bodies of small-sized Hypophyllus hydrangeas. , the method of collecting tissues and then extracting DNA, but this will lead to the loss of a large number of good individuals with sufficient genetic information, and the obtained individual genetic information has no practical significance in terms of species selection

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  • DNA (deoxyribonucleic acid) in-vivo sampling method for young Hyriopsis cumingii
  • DNA (deoxyribonucleic acid) in-vivo sampling method for young Hyriopsis cumingii
  • DNA (deoxyribonucleic acid) in-vivo sampling method for young Hyriopsis cumingii

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[0038] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0039] In the present embodiment, the method for sampling the living body DNA of the young clam of the triangular sail clam comprises the following steps:

[0040] 1> Stripping of mantle tissue of juvenile clam:

[0041] Select 20 juvenile mussels about 50 days after seedling removal, with a body length of 1.7-2.1cm and a body weight of 243-391mg, and divide them into an experimental group and a control group, with 10 juveniles in each group. Corresponding to the mark, use a vernier caliper to measure and record the body length, and measure and record the body weight; use a blade with a width of about 5 mm and a certain toughness to gently pry open the mussel shell of the young clam in the experimental group by 2-3 mm, and cut 2 mg of the man...

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Abstract

The invention discloses a DNA (deoxyribonucleic acid) in-vivo sampling method for young Hyriopsis cumingii. The research object of the method is small in size, and does not need to be put to death; the economic character of the object can be bred at the earlier stage to enhance the breeding efficiency; the consumption of tissue materials for DNA extraction is extremely low; and the extracted DNA is good in quality, thereby meeting the requirements of biological research. The technical key points of the invention are as follows: the DNA in-vivo sampling method for young Hyriopsis cumingii comprises the following steps: 1) peeling mantle tissues of young Hyriopsis cumingii; 2) extracting the genome DNA of the peeled tissues; 3) detecting the extracted DNA; and 4) culturing the young Hyriopsis cumingii.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a DNA sampling method of juvenile spinosa mussel DNA. Background technique [0002] Triangle sail mussel is a unique freshwater mother-of-pearl mussel in China. Breeding a triangle sail mussel with good quality is one of the key problems that need to be solved at present. The use of molecular biology methods (such as molecular marker technology, etc.) can effectively carry out genetic breeding and germplasm improvement of S. sinensis. [0003] Extracting a certain quality of high-concentration genomic DNA is the basis and premise of molecular biology research. It is relatively simple to extract DNA from living adult Hypophyllus sinensis, but it is very difficult to extract DNA from living bodies of small-sized Hypophyllus hydrangeas. , to collect tissues and then extract DNA, but this will lead to the loss of a large number of good individuals with sufficie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 罗明李家乐白志毅郭诗照
Owner SHANGHAI OCEAN UNIV
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