Marking method of selective breeding individual of turbot

Abstract

The invention relates to a marking method of a selective breeding individual of turbot. The method comprises the following steps of: firstly, selecting a glue needle, a hangtag gun and a mark board, wherein the glue needle is 10mm and in a transverse H shape, the model number of the glue needle is MUXP-25, the model number of the hangtag gun is SAGA 70L, the mark board is made of Solisep white polyester composite paper, the sign board has a length of 2 centimeters and a width of 0.8 centimeter, and a mark code is a 5-bit identifier; secondly, selecting a turbot juvenile fish the body length of which is longer than 5 centimeters, and keeping the fish to have a swimming posture during marking; positioning an inserting point at a part having more muscles behind the left eye on the head of the fish body; reserving a length of 2-8 millimeters of the glue needle in vitro; and finally, putting the marked fish in a 10ppm florfenicol medicated bath for 2 hours, carrying out the medicated bath once each day and continuously carrying out the medicated bath for three days. The method has simple operation, low cost, high automation degree and smaller damage to the fish body. The retention rate of a mark is higher than 90 percent, and the effective recognition rate is higher than 90 percent. The normal activity of the turbot cannot be influenced, and the method can meet the requirements of the large-scale breeding and marking of the turbot.

Description

Technical field: [0001] The invention belongs to the fish individual marking technology in the fields of fish breeding, population estimation, resource evaluation, and migration and distribution investigation, and is a technical method for identifying a large number of fish and detecting each fish in turbot breeding. Background technique: [0002] Turbot (Scophthalmus maximus L.) is a flounder endemic to the northeastern Atlantic Ocean, off the coast of Europe. my country introduced turbot seedlings from the UK for the first time in 1992. Because the broodstock used in the production of turbot seedlings in my country is usually not scientifically selected, coupled with repeated generations of breeding and inbreeding, the germplasm of offspring seedlings is degraded. The phenomenon is relatively serious, and the hatching rate and emergence rate have decreased, the growth rate has slowed down, and the stress resistance has deteriorated. For this reason, it is necessary to carry...

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Problems solved by technology

Ma Aijun of the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences and others applied for "A Marking Method for Broodstock Based on Selective Breeding of Turbot" (Application No.: 200810083837.1), mainly using red copper as the material to make marking numbers and putting them in liquid nitrogen tanks Let it stand for 3 minutes, take it out and put it on the marked part of the fish body for 2 minutes; the cost of the present invention is low, the operation is simple, the effect is remarkable, and it is easy to observe; but this patent also requires marking after the fish body grows to a certain size, And because the turbot grows fast, it is difficult to ensure that the marked numbers are clearly visible after a long time, thereby reducing the effective identification rate of the marked; and red copper is used as the material to make the marked numbers (the marked numbers of each individual are different, so it is necessary to have marked numbers The number of red copper molds is very large) the cost is high

"Research Progress in Fish Marking Technology" by Zhang Tanglin, Institute of Hydrobiology, Chinese Academy of Sciences (Chinese Fishery Science / 2003 / Volume 10 / No. 3) and the patent applied by Dong Zaijie, etc., Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences A method for individual marking of tilapia" (application number: 200810018667.9), in which a large number of individual marking methods are mainly to inject PIT marking chips into the fish for individual marking; however, affected by the size of the PIT marking chip, this marking The method requires that the fish grow to a certain size (generally used in the marking of adult fish); and the current market price of PIT marking chips is relatively high (price: a PIT marking chip, domestically produced is about 20 yuan; foreign imports are more than 30 yuan) , the cost of selective breeding for large-scale turbot is undoubtedly too high

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