The invention discloses a formula and a preparation process of povidone iodine gel for veterinary use, and particularly relates to povidone iodine gel for veterinary use and a preparation process. The prescription of the medicament consists of povidone iodine, a moisturizing lubricant, a gel matrix, a stabilizer, buffer solution and sterilized water. According to the preparation process, the PH value and the proportion are adjusted to process the povidone iodine gel. The povidone iodine in the gel has the advantages of high bactericidal power, wide antibacterial spectrum, small stimulus, no allergenicity, low toxicity, no drug resistance, no influence on quality of lactation milk, no milk withdrawal period and insensitivity of a sow to the smell of the gel; after the povidone iodine is prepared into the gel, effective iodine of the povidone iodine is more stable to fulfill the aims of slow release and high efficiency; the moisturizing lubricant is added into the gel, so that the povidone iodine is close to the wall of uterus in the uterus and is easier to absorb; and through the stabilizer and the buffer solution in the gel, the effective iodine is stable, and the influence of external environment is avoided.