The invention discloses a bunching screw automatic locking device of multi-axle buffering. The device is characterized in that the device comprises a machine body (2), an air cylinder (7) and an upper movable board (1), wherein the air cylinder (7) is arranged on the machine body (2), the upper movable board (1) is connected onto the flexible rod of the air cylinder (7), and the upper movable board (1) is respectively provided with a limit mechanism and at least two locking mechanisms. The bunching screw automatic locking device is simple in structure and low in cost, the at least two locking mechanisms are arranged, and the automatic locking of bunching screws is realized, so that the assembling production efficiency is greatly improved, the labor productive force cost is reduced, and the production quality is improved; the limit mechanism can limit the movement of the locking mechanisms close to the screw position of a workpiece, the device can be prevented from damaging the workpiece, and the device is suitable for popularization and use.