Double-layer collagen-chitosan sponges bracket and method of preparing the same

Abstract

The invention discloses a dual-layer collagen-chitosan sponge scaffold and a preparation method thereof, and relates to a tissue engineering scaffold. The invention provides the dual-layer collagen-chitosan sponge scaffold for the tissue engineering skin and the preparation method thereof. The scaffold is a dual-layer mesh structure, the upper and the lower layers are collagen and chitosan composite films, and the hole diameters of the layered and hole-shaped structure are mutually penetrated. The collagen hydration solution is dissolved in acetic acid for preparing the collagen dispersion solution with the concentration of 0.2 percent to 0.5 percent and 0.5 percent to 0.8 percent; the chitosan is dissolved in the acetic acid for preparing the chitosan dispersion solution with the concentration of 0.2 percent to 0.5 percent and 0.5 percent to 0.8 percent; the two dispersion solutions are mixed to obtain two collagen-chitosan solutions; the collagen-chitosan solution with the concentration of 0.5 percent to 0.8 percent is frozen overnight, the sponge-like white scaffold is obtained by drying, and the single-layer scaffold is obtained by cross-linking after the film pressing, cleaning, freezing overnight and drying; the collagen-chitosan solution is poured in for freezing overnight and drying to obtain the dual-layer scaffold, and the cross-linking, the cleaning, the freezing overnight, the freezing and the drying are carried out.

Description

technical field [0001] The invention relates to a tissue engineering scaffold, in particular to a double-layer collagen-chitosan sponge scaffold used as a scaffold material for skin tissue engineering and a preparation method thereof. Background technique [0002] Tissue engineering scaffolds are used to simulate the porous structure of the extracellular matrix to endow the scaffold with some basic functions of the extracellular matrix. At the same time, the scaffold material is gradually degraded and absorbed, thereby inducing the directional migration of seed cells, damaged tissue or organ cells to achieve repair The purpose of a tissue or organ. Tissue engineering scaffolds are key factors in the formation of tissue engineered tissues and organs. An ideal tissue engineering scaffold should have: (1) Good biocompatibility and biodegradability, no obvious inflammatory response, immune response and cytotoxicity, and the degradation and absorption speed in vitro and after im...

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Problems solved by technology

Most of the reported double-layer skin scaffolds are made of polyurethane or chitosan, and there are no reports of collagen-chitosan double-layer sponge scaffolds ([3] Matsuda K, Suzuki S, Isshiki N, et al. Re- freezedried bilayer artificial skin.Biomaterials[J].1993,14:1030-1035;[4]Fwu-Long Mi, Yu-Bey Wu, Shin-Shing Shyu, et al.Asymmetric chitosan membranes prepared by dry / wet phase separation: a new type of wound dressing for controlled antibacterial release [J]. Journal of MembraneScience, 2003, 212: 237-254; [5] Jianbiao Ma, Hongjun Wang, Binglin He, et al. A preliminary invitro study on the fabrication and tissue engineering applications of a novel chitosan bilayer material as a scaffold of human neofetal dermal fibroblasts [J]. Biomaterials, 2001, 22: 331-336)

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