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Tissue engineering skin capable of regulating colouring matter secretion and its construction method

A tissue-engineered skin and construction method technology, applied in artificial cell constructs, tissue culture, biochemical equipment and methods, etc., can solve problems such as imperfect tissue engineering products, and achieve repair of full-thickness skin defects with good vitality and structure full effect

Inactive Publication Date: 2004-05-05
陕西艾尔肤组织工程有限公司
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

At present, although tissue engineering methods have made great progress in the development of skin, mucous membrane, muscle, bone (and cartilage), blood vessels, nerves and cornea, some products have been applied clinically, and have achieved gratifying therapeutic effects and markets. benefit, but the tissue engineering products that have been or are under development are still not perfect. Most of the tissue engineered skin substitutes reported so far are quite different from normal tissues. As mentioned above, there are no hair follicles, blood vessels, sweat glands and melanin in normal skin. Cells, Langerhans cells, etc.
There are many defects in the maturity of the method, the development cycle and the cost of the product.

Method used

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  • Tissue engineering skin capable of regulating colouring matter secretion and its construction method
  • Tissue engineering skin capable of regulating colouring matter secretion and its construction method
  • Tissue engineering skin capable of regulating colouring matter secretion and its construction method

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Embodiment Construction

[0028] Below in conjunction with experiment the inventive method is described in further detail:

[0029] 1. Isolation and cultivation of each cell:

[0030] (1) Get the foreskin tissue after the circumcision of newborns or adolescents, in order to ensure sterility, soak 1 minute in 75% ethanol before processing;

[0031] (II) Transfer the foreskin tissue into a culture dish, wash it with PBS buffer solution added with antibiotics, trim it with scissors, remove the subcutaneous tissue, and cut the tissue into 2mm×10mm with a blade;

[0032] (III) Put 4 pieces of tissue into a centrifuge tube, add 4ml of skin separation solution, and digest at 37°C for 2.5 hours; if cold digestion is used (recommended), dilute the above separation solution with PBS, add 4ml, and heat at 4°C Digest for 14-16 hours;

[0033] (IV) Put the digested skin into a petri dish, and carefully separate the epidermis and dermis with forceps (extreme care must be taken to avoid bringing the dermal tissue i...

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Abstract

A tissue-engineered artificial skin able to regulate the pigment secretion is configured through externally culturing the ceratin cells, fibroblasts and melanocyte of skin, proportionally mixing by tissue engineering method, and forming artificial skin.

Description

technical field [0001] The invention belongs to the technical field of constructing artificial organs by tissue engineering in biomedical engineering, and relates to a tissue-engineered skin capable of regulating pigment secretion and a construction method thereof. Background technique [0002] Tissue engineering is currently a research hotspot in the field of life sciences, and related research involves various aspects. In recent years, with the development of tissue engineering technology, research reports on the development of artificial skin substitutes have gradually increased, especially the development of different types of artificial skin through tissue engineering technology, the core of which is the combination of applied engineering principles and methods with life sciences , to develop biological substitutes with restoration, maintenance or improvement functions. [0003] Skin is an important tissue and organ that covers and protects the body surface. Defects a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60C12N5/071
Inventor 金岩刘源赵宇
Owner 陕西艾尔肤组织工程有限公司
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