117 results about "Melanophore" patented technology

This invention relates to monoclonal antibodies that recognize an antigen specific to melanocytic lesions. These antibodies are useful in methods of isolating melanoma cells and diagnosing melanocytic lesions. These antibodies are also useful for immunotherapy against melanoma.

The invention relates to a pigment-containing artificial skin detection model and a preparation method thereof. The artificial skin detection model is an epidermal layer structure with activity, which is constructed by compositely inoculating cutin formative cells and melanophores to the surface of a support membrane made from a biodegradable material through culture and comprises a stratum basale, a stratum spinosum, a stratum granulosum and a stratum corneum, and a basilar membrane structure is formed between the epidermal layer and the biodegradable material positioned below the epidermal layer. Compared with the conventional product, the invention can better simulate the pigment components of natural skin and more accurately reflect the whitening and sunscreen effect of cosmetics because of containing the melanophores, nourish the cutin formative cells by using the support membrane made from the biodegradable material as an enderonic layer, control the contraction of the epidermal layer with the strength and enhance the stability of the detection model and has low raw material cost, short manufacturing period and mass production; and in addition, the prepared artificial skin detection model can be directly used for the efficacy detection of the cosmetics and the skin irritation detection of textiles, the cosmetics and chemicals.

An in vitro skin equivalent includes at least one epidermis equivalent and at least one dermis equivalent, and further includes melanocytes constitutively producing melanin and fibroblasts.

The invention discloses a tissue engineering material-based culture method and applications of melanophore. In the method of the invention, the tissue engineering technology is used to build carrier material with good biocompatibility and perform culture (co-culture) and transfer of melanophore or melanophore, fibroblast and keratinocyte and the method can be used in depigmentation diseases such as vitiligo and for the regulation of skin color. The invention relates to the preparation of the carrier material with biocompatibility and the building and transplanting of the cell-carrier composite material. The carrier material can be used to provide good carrier for cell culture, maintain cellular activity and solve the problem that the cell suspension is easy to lose in the cellular transplantation process; the inoculum density and culture time of cells can be regulated at the same time, the regulation to the skin colors of different individuals can be realized; and the carrier materialhas good performances such as damage resistance, tear resistance and easy transfer, has good function of promoting wound healing, and satisfies the use requirements of the large-area depigmentation disease and the regulation of skin color.

The present invention relates to an inhibitor for melanin which contains tranexamic acid and niacinamide as active ingredients for inhibiting the formation of melanin cells in the skin, and a cosmetic composition containing the inhibitor for melanin as an active ingredient for relieving liver spots, blemishes, freckles and inflammatory hyperpigmentation, improving skin tone and texture, and skin whitening.

A tissue-engineered artificial skin able to regulate the pigment secretion is configured through externally culturing the ceratin cells, fibroblasts and melanocyte of skin, proportionally mixing by tissue engineering method, and forming artificial skin.

The invention provides a culture method for acquisition of tissue-engineered epidermis and application of the culture method. By means of co-culture of epidermal cells and melanocytes, an epidermis membrane with a stratum bassale, a stratum spinosum, a stratum granulosum and a stratum corneum can be acquired, and large area, avoidance of rejection reaction, structural similarity to natural epidermis, excellent treatment effects and the like are realized.

The present invention discloses a whitening and moisture supplementing mask, which contains (1) a mask base cloth, and (2) a mask essence, wherein the mask essence comprises (a) a water-soluble thickener, (b) a humectant, (c) a whitening composition and (d) a water-containing carrier, wherein the humectant comprises a polyol, sodium hyaluronate and hydroxyethylurea, and the whitening composition comprises papain, a narcissus bulb extract and a tropaeolum majus extract. According to the present invention, the whitening and moisture supplementing mask can accelerate the peeling of old and waste skin cutin, promote the skin surface layer regeneration, inhibit the tyrosinase activity, inhibit the melanocyte division, reduce the melanogenesis, and achieve the good whitening effect from multiple angles.

The invention relates to an epidermal tissue culturing method, an epidermal tissue culture prepared by using the method and application of the epidermal tissue culture in the field of cosmetic dermatology. The epidermal tissue culturing method comprises a step of culturing a culturing mixture of melanin cells and epidermal keratinocytes under the conditions that the quantity of the epidermal keratinocytes is 100 and the quantity of the melanin cells is in a range of 0.01 to 50.

The invention discloses a whitening skin care product. The whitening skin care product contains the following whitening ingredients: nicotinamide, acetyl chitin, 3-O-ethyl-ascorbic acid, hydroxydecylubiquinone, 4-(alpha-methylbenzyl)resorcinol and resveratrol, and the whitening ingredients can be combined to play the whitening effect from multiple paths, wherein hydroxydecyl ubiquinone and acetylchitin can resist ROS produced by external stimuli (such as: UV irradiation, lack of sleep, environmental pollution, and the like); 3-O-ethyl-ascorbic acid and 4-(alpha-methylbenzyl)resorcinol can inhibit enzymes required for producing melanin; 3-O-ethyl-ascorbic acid also can inhibit polymerization of melanin into large melanin polymers, and thereby evens skin tone; nicotinamide can inhibit thetransfer of melanin from melanocytes to keratinocytes to prevent skin from developing and blackening, and makes melanin be digested and swallowed in the melanocytes; and resveratrol can reduce melaninthat has been formed to lighten spots. Combining with a formula design of the invention, the whitening essence of the whitening skin care product can effectively whiten skin, lighten spots and even skin tone.

The invention provides a culture medium for in vitro culture of epidermal melanophore. In the culture medium, a basic culture medium which is suitable for in vitro culture of the epidermal melanophore is used as a substrate, and growth factors (1), cAMP accelerant (2) and serum (3) are added into the substrate. The invention has the beneficial effects that the invention provides the culture medium which is specially used for in vitro culture of the epidermal melanophore, and the culture medium can be used for culturing epidermal melanophore with strong propagation capacity, migration capacityand melanin synthesizing capacity, can enable melanophore of patients to better grow and differentiate in vitro, and can be used for transplantation treatment of depigmented or nonpigmented dermopathic patients.

The present invention discloses a melanin-containing skin models in vitro skin test model and a preparation method and use. The test model comprises a dermis layer and an epidermis layer, the epidermis layer comprises epidermal cells and melanin cells, and the dermis layer is a membrane comprising fibroblast and an extracellular matrix formed by the fibroblast. The skin model is very similar to the human skin structure, the building process simulates the skin development process, the epidermis layer can be developed under nourishing of the dermis layer, and synthesis, secretion, transport and the like of melanin can be promoted. The present invention discloses a preparation method and use of the skin test model in whitening and freckle removal effect testing of a variety of cosmetics, health products and pharmaceuticals, related mechanism exploration and research, and the like.

This invention relates to monoclonal antibodies that recognize an antigen specific to melanocytic lesions. These antibodies are useful in methods of isolating melanoma cells and diagnosing melanocytic lesions. These antibodies are also useful for immunotherapy against melanoma.

The invention discloses a method for preparing a composite membrane for treating leucoderma from autologous follicle melanocytes. Enough autologous melanocytes are separated from follicles of a patient; cells are transferred to a biological membrane to continually grow by using the characteristics that performances and functions of the autologous melanocytes changed after the autologous melanocytes are cultured and amplified, so that the controllability of the cells is effectively improved and clinicians can extremely conveniently use the composite membrane. The defects of a traditional leucoderma treatment surgery that the damages are great, the treatment time is long, the treated skin lesion face is not large, the surgery is not suitable for treating infants and children, and scars are formed and the like are avoided. The method is convenient to operate, has good repeatability and is suitable for large-scale popularization of clinic treatment of the leucoderma.

The present invention is a system and methods of establishing a Melanocyte Protection Factor (MPF), which indicates the level of protection against DNA damage to a target cell, such as the level of protection a particular sunscreen offers against UVA rays when compared to the unprotected case, i.e., no sunscreen. The present invention determines and records levels of stable melanin radicals (SMR) in a target cell. Light is applied to the target cell forming light-induced melanin radicals (LIR). The levels of SMR and intensity of LIR are measured to determine the amount of incident light reaching the target cell. Since LIR is proportional to the square root of light intensity reaching the target cell, the ratio of light reaching the target cell is defined as the MPF: MPF=UV⁢ ⁢reaching⁢ ⁢melanocytewithout⁢ ⁢sunscreenUV⁢ ⁢reaching⁢ ⁢melanocytewith⁢ ⁢sunscreen=[SMR+screenLIR+UV+screen]2[SMRcontrolLIR⁢+UV⁢ ⁢control]2

The invention relates to a melanin extraction method from a black-bone silky fowl, which comprises the following steps: the black-bone fowl is cut up and washed by using 1 M to 10 M of hydrochloric acid solution for 5 minutes to 30 minutes and is immerged into liquid nitrogen for 0.5 minute to 5 minutes; after that, the black-bone fowl is grinded into 60 meshes to 100 meshes powder and transferred into a Soxhlet extractor to do thermal reflux for 1.5 hours to 3 hours by using 1 M to 10 M of hydrochloric acid; after the leaching liquor is cooled to 0 DEG C to 5 DEG C to be precipitated; acetone distilled water, which has the same volume with the leaching liquor, is respectively added into the precipitate for washing for 3 to 4 times, drying, then the melanin is made. Through the grinding process after the liquid nitrogen is frozen, the method of the invention which can fully and effectively damage the structure of melanophore in tissue under the precondition of no changing the physical chemistry properties of the melanin, is in favor of fast melanin release, shortens melanin extraction time and keeps nearly complete the melanin morphological structure of the black-bone fowl.

The invention relates to skin aging resistant cosmetic, which belongs to the field of cosmetics. The skin aging resistant cosmetic is prepared by the following steps of decocting, filtering and concentrating dendrobium officinale, radix paeoniae alba, bletilla striata, gingko, tuckahoe, ligusticum wallichii, pericarpium citri reticulatae, salvia miltiorrhiza, radix curcumae, caulis spatholobi, schisandra chinensis, purple perilla and cuscuta Chinensis. The skin aging resistant cosmetic has the aging resistant function and has the effects of inhibiting melanophore and locke neuraminidase and resisting free radicals.

The invention provides an oriental cherry flower extract and an extraction method and application thereof. The extraction method comprises the following steps: extracting oriental cherry petals by adopting an extracting agent to obtain the oriental cherry flower extract, wherein the extracting agent comprises a composition of C3-C6 small molecular polyhydric alcohols, ethanol and water. The extraction method is simple in process route, large-scale industrial application is easily realized, and the obtained oriental cherry flower extract contains rich high-activity active ingredients, has an excellent hair-blacking effect, can be applied to hair-blacking cosmetics of multiple dosage forms such as aqueous solution and emulsion and is wide in application range. The oriental cherry flower extract is mild and non-irritating, is capable of activating tyrosinase, repairing damaged melanocytes and promoting growth of the melanocytes and generation of melanin, regulation and repair are performed from a melanin formation pathway, the aims of blacking hair and improving gray hair and white hair are achieved, the hair can be effectively blacked after the oriental cherry flower extract is usedfor 15 days, and the oriental cherry flower extract has excellent effects.

The invention relates to a cosmetic, and belongs to the field of cosmetics. The cosmetic is prepared from dendrobium officinale, radix paeoniae alba, bletilla striata, gingko, poria cocos, ligusticum wallichii, pericarpium citri reticulatae, rhizoma atractylodis, coptis chinensis, radices lithospermi, rhizoma polygonati, semen cassiae and radices sileris through the steps of decocting, filtering, concentrating and the like. The cosmetic with an anti-aging function, disclosed by the invention, has the effects of restraining melanophore, restraining tyrosinase and resisting free radicals.

The invention discloses a construction method for an in vitro skin test model containing melanocytes. The construction method comprises the following steps: using a culture medium for human epidermal keratinocytes as a basic liquid, and respectively preparing a culture medium a and a culture medium b; respectively preparing the keratinocytes and the melanocytes into cell suspensions by adopting the culture medium a, respectively and uniformly inoculating into a Transwell chamber, then adding the culture medium a, carrying out immersed culture for 1-5 days in a 5% CO2 incubator at the temperature of 37 DEG C, and forming an epidermal layer structure containing the melanocytes; placing on the surface of a culture support in a culture dish, adding the culture medium b, carrying out gas-liquid level culture, putting into the 5% CO2 incubator at the temperature of 37 DEG C and culturing for 5-15 days, so that the skin test model containing the melanocytes is obtained. The construction method disclosed by the invention has the advantages that model stability is high, the melanocytes are more sensitive to external stimulation, the model can be accurately used for detection on safety and efficacy assessment of cosmetics, raw material cost is low, production cycle is short, stability is good, and the model can be produced in quantity.

The invention discloses plant-extracted hair-blacking essence. The essence is characterized by comprising the following raw materials of water, ethanol, a radix scutellariae extract, a fructus viticisextract, a polygonum multiflorum extract, a tea extract, a ginseng extract, a leonurus extract and methylisothiazolinone. Compared with the prior art, the invention has the following characteristicsthat the adopted natural traditional Chinese medicine extract has no toxic or side effect, is mild and non-irritating, capable of effectively supplementing melanin required by hair, deeply permeatinghair roots, repairing damaged melanocytes and promoting natural generation of melanin, thereby effectively playing a role in blackening and brightening hair; the nutrition required by scalp can be supplemented, and the problem of scalp aging caused by malnutrition of the scalp can be improved; and the essence can supplement nutrition required by scalp, effectively clean and purify the scalp, repair the scalp and balance water-oil conditions of the scalp.

The invention relates to the field of medical cell therapy, in particular to a device and a method for rapidly and immediately separating epidermal cells, melanophore and fibroblast of human skin. The separating device comprises a micro magnetic heating stirrer, an operating panel, digestive enzyme and an isotonic electrolyte solution. According to the separating method, instruments required for cell separation of the human skin are remarkably reduced, the operation steps are simplified, time required for cell separation is remarkably shortened, application of serum is avoided, occurrence of serious complication such as allergy, pathogenic microorganism propagation and the like is remarkably reduced, and wide popularization of human skin cell transplantation application is facilitated. According to the device and the method, separated cells can be used for scar plastic surgery, treatment of abnormal pigment change such as vitiligo and scar hypopigmentation caused by various reasons and acute and chronic wound repair.

The invention relates to an isolated culture method for primary generation rabbit melanophore. According to the method, the rabbit melanophore is isolated through an enzyme digestion method, and after culturing is conducted through an M254 complete medium, an M254 / HMGS culture medium is adopted through replacement for culturing till the convergence degree reaches 80%-90%, then a differential digestion method is utilized for removing other cells, and the purified melanophore is obtained. The purity of the melanophore is greatly improved, the cultured cells are identified through cell morphologic observation, L-DOPA staining, semiquantitation and an anti-S-100 immunocytochemical staining method, the result shows that the isolated primary generation melanophore is mostly in a dendritic shape and a thin and long shuttle shape, the brownish black is shown after L-DOPA staining, the RT-PCR analysis shows that the isolated cells express the marker gene of the melanophore, and the S-100 staining cytoplasm is brown.

The invention is related to iris color changing method that ensures a color change in iris by shedding the layer that contain the cells with melanin pigments in the upper superficial part and stroma of the iris, colored part of the eye, through photo-disruptive effect.

An elapsed-time determination apparatus includes: a storage unit that stores correlation information indicating a correlation between a state of a melanophore of a fish and an amount of time elapsed since the death of the fish; an acquisition unit that acquires an image of a fish; an analysis unit that detects a state of a melanophore of a fish by analyzing an image acquired by the acquisition unit; and a determination unit that determines the amount of time elapsed since the death of the fish in accordance with the state of the melanophore of the fish detected by the analysis unit, based on the correlation information, and outputs determination results.

The invention discloses a white hair-to-black hair essence and a preparation method thereof. The white hair-to-black hair essence comprises the following components in parts by weight: 80-100 parts ofdeionized water, 0.1-1.2 parts of hydroxyacetophenone, 0.1-0.8 part of 1,2-hexanediol, 0.1-0.6 part of citric acid, 0.1-0.6 part of sodium citrate, 1-8 parts of modified European larch wood extracts,1-6 parts of extracts of Chinese arborvitae branchlet tips and leaves and 0.1-2 parts of plankton extracts. The modified European larch wood extract is a stable antioxidant, plays a role in promotingstem cell proliferation and maintenance, and can be combined with a black-haired precursor n-acetyl-tyrosine to stimulate hair follicle stem cells to produce new melanocytes and activate melanin production; and the essence can delay the oxidation and disappearance of melanin while stimulating the generation of melanin in hair follicles, thereby achieving the effect of turning hair from white to black.

The invention provides sunscreen and after-sun repairing skin care composition and a cosmetic containing the same. The composition comprises a rhEGF (recombinant human epidermal growth factor), hydrolyzed soy protein, purslane herb extract, Chinese magnoliavine fruit extract and rhizoma kaempferiae extract. The weight content of the composition in the cosmetic is 1%-2%. The weight content of the rhEGF in the sunscreen cosmetic is only 1-10 mu g / ml and is far lower than the conventional addition amount, the rhEGF is compounded with the hydrolyzed soy protein, the purslane herb extract, the Chinese magnoliavine fruit extract and the rhizoma kaempferiae extract to realize synergistic effect, so that injured cells can be rapidly repaired, skin injury caused by ultraviolet radiation is relieved, further, abnormal increase of melanophores of a skin basal layer can be reduced, melanin synthesis is blocked, black spots growing on the after-sun skin are reduced, gene mutation factors of the injured cells are eliminated, light aging is prevented, and accordingly, the composition has an ultraviolet ray preventing function and an after-sun injury repairing function.

The invention provides freckle removal cream and a preparation method. The freckle removal cream is prepared from 0.01%-0.1% of radix scutellariae extract, 0.005%-1% of radix glycyrrhizae extract, 0.005%-0.2% of grape seed extract, 0.0005%-0.001% of coenzyme, 2%-8% of olive fruit oil, 0.05%-1% of tocopheryl acetate, 2%-7% of arbutin, 2%-7% of magnesium ascorbyl phosphate, 1%-4% of Jojoba oil, 2%-8% of isopropyl palmitate, 2%-8% of glycerin, 2%-5% of cyclomethicone, 3%-9% of propylene glycol, 2%-7% of glyceryl stearate, 0.1%-0.5% of imidazolidinyl urea, 0.1%-0.4% of methylparaben, 0.05%-0.14% of propylparaben, 2%-5% of stearic acid, 2%-6% of stearyl alcohol, 2%-6% of potassium C9-15 alkyl phosphate, 0.1%-1% of essence and the balance of deionized water. The freckle removal cream has no sideeffect and can retard activity of melanophores and nurse the skin.

The invention provides a composition for skin, application of the composition, a whitening and brightening cosmetic composition and a preparation method of the whitening and brightening cosmetic composition, and relates to the technical field of cosmetics. The skin composition comprises verbascoside, nicotinamide and a lactic acid bacteria fermentation lysate, wherein the verbascoside is mainly used for inhibiting formation of melanin particles, the nicotinamide is mainly used for inhibiting melanin from being transmitted to keratinocytes, and the lactic acid bacteria fermentation lysate is mainly used for accelerating shedding of melanin-containing cells in a cuticle. The verbascoside, the nicotinamide and the lactobacillus fermentation lysate are matched and have a synergistic effect, so the problems of melanin content, reduction and / or elimination of skin color change caused by hormone or environmental factors such as ultraviolet rays in a human body can be solved from a multi-dimensional and multi-channel target spot; therefore, the effects of effectively brightening the skin color and reducing the melanin content are achieved. The invention further provides application of the composition for skin and a whitening and brightening cosmetic composition.

The invention discloses a fat-soluble glabridin compound whitening agent and application of the agent in cosmetics. The compound whitening agent is prepared from the following active ingredients in byweight percent: 30-50 percent of glycyrrhiza glabra extract, 10-30 percent of tetrahydroxychalcone, 10-30 percent of ubiquinone and 10-30 percent of an endothelin antagonist. The compound whitening agent has main whitening action mechanisms of inhibiting the activity of tyrosinase, inhibiting the activity of DOPA chrome tautomerase and obstructing indole-5,6-quinone polymerization, so that melanogenesis can be obstructed, differentiation of melanophores can be reduced, and the aims of whitening skin, lightening spots and improving dark skin color can be achieved. The compound whitening agenthas a simple and practical formula and a good whitening and spot-lightening effect, is environmentally friendly and safe since natural products are adopted, is mild and nonirritant in use, and can overcome the defect in an existing whitening agent.