27 results about "Melanophore" patented technology

The invention discloses a polypeptide-nucleic acid tetrahedron compound as well as a preparation method and application thereof. The compound comprises a nucleic acid tetrahedron and polypeptide, wherein the polypeptide is nonapeptide-1 with an amino acid sequence as shown in SEQ ID NO: 13; the nucleic acid tetrahedron is formed by self-assembly of four single-stranded DNAs with the same length. According to the compound, the transdermal function of nucleic acid tetrahedron capable of inhibiting melanogenesis and the melanocyte targeting function of nonapeptide-1 are combined, so that the TDN-NA-1 compound has the melanocyte targeting property while having the autonomous transdermal ability, and the blackness inhibiting effects of the nucleic acid tetrahedron and the nonapeptide-1 are combined, so that the effect of inhibiting melanogenesis is achieved. Therefore, the whitening composition has a stronger capability of inhibiting melanin generation, so that the purpose of precise whitening is achieved, and the whitening composition has a wide application prospect in the field of cosmetics.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention discloses a whitening composition as well as a preparation method and application thereof. The whitening composition is prepared from the following components: a tea leaf extract, a lavender flower extract and water, wherein the tea leaf extract, the lavender flower extract and the water are added into the whitening composition. The whitening composition can interact and synergistically improve the autophagy ability of cells, synergistically improve the phagocytosis of melanin in keratinocytes and the removal ability of skin metabolic wastes, can synergistically inhibit the generation of melanin and synergistically improve the expression of miR-137 genes in melanocytes, has a relatively high development prospect of cosmetics with whitening effects, and has a wide application prospect. Moreover, the composition is mild in property, easy to absorb and non-irritant, and can be widely applied to skin care cosmetics.

This invention discloses a skin-whitening composition that reduces aging melanocytes and its application, relating to the cosmetics field. The invention provides a composition that, by reducing aging melanocytes and decreasing SASP secretion, reduces melanin production and inhibits tyrosinase activity while maintaining healthy melanocytes, thus maintaining skin homeostasis while whitening the skin. This composition can be widely used in various skincare products to effectively achieve long-lasting whitening effects.

The application discloses a method for regulating body color of ornamental fish and belongs to the technical field of aquaculture. The application finds that, by using thyroid hormone to soak ornamental fish in the fry period and the juvenile fish period, excessive thyroid hormone can significantly reduce the number of red pigment cells and the size of pigment granules of ornamental fish in different periods, and reduce the content of carotenoids; when the synthesis of thyroid hormone is inhibited, the expression of melanin cells and related synthesis pathway genes is promoted, and the carotenoid level is increased. The application first clearly defines the inhibitory effect of thyroid hormone on the development of red pigment cells and the accumulation of carotenoids of gourami, fills the knowledge gap in the field, and provides important guiding information for future regulation of body color and genetic improvement of ornamental fish and other aquatic animals.

The invention relates to the field of cosmetics, in particular to a compound and application thereof. The inventor creatively discovers that the compound with the structure shown in the formula I has the effect of inhibiting melanocytes and tyrosinase, and proves that the compound has new application in whitening and has application in preparation of whitening products. Experiments show. The compound with the structure shown in the formula I is superior to the traditional capsaicin with a whitening effect in the inhibition effect on melanocytes and tyrosinase.

The application discloses a white hair to black hair essence and a preparation method thereof. The white hair to black hair essence is prepared from selected raw materials, such as water dropwort leaf / stem extract, oxyhematin, radix polygoni multiflori, panax notoginseng and the like, and has good effects on activating hair follicles, enhancing the activity of melanocytes in the hair follicles and promoting melanin production. Volunteer tests prove that with the growth of new black hair and the shedding of aging white hair, the black hair gradually increases and the white hair gradually decreases by long-term use of the white hair to black hair essence, and the sensory effect of 'white hair to black hair' is achieved.

The invention relates to the technical field of cosmetics, in particular to a mark fading and repairing composition as well as a preparation method and application thereof. The invention relates to a mark fading and repairing composition which comprises the following components in percentage by weight: 0.1%-5% of dragon's blood extract, 0.01%-0.5% of keratinase and 1%-5% of nicotinamide, the preparation method comprises the following steps: preparing the eutectic solvent; performing ultrasonic-assisted extraction; separation and post-treatment; the invention has the following beneficial effects: the resina draconis extract can inhibit continuous pigmentation caused by acne inflammation residue, promote healing of skin minimal wounds and reduce dermis layer injury of acne mark parts; keratinase specifically hydrolyzes stratum corneum aged keratin, cutin metabolism of acne mark parts is accelerated, and accumulation of pigments in stratum corneum is avoided; nicotinamide inhibits melanocytes from transferring melanin to keratinocytes; through the synergistic effect of the three components, a closed-loop printing fading mechanism of anti-inflammatory repair-keratin metabolism-pigment inhibition is formed, the printing fading effect is more comprehensive and more efficient, and skin repair and barrier protection can be both considered.

The application discloses a method suitable for depigmentation of melanoma tissue in immunohistochemical staining, and the method comprises the following steps: after an antigen repairing step in the immunohistochemical staining process, a depigmentation operation is performed; a hydrogen peroxide methanol solution is used as a depigmentation solution; and the depigmentation is performed through heating incubation, wherein the heating temperature is 55 DEG C to 75 DEG C, the volume fraction of the hydrogen peroxide methanol solution is 5% to 15%, and the incubation time is 10 to 20 minutes. The method simplifies the depigmentation process of melanin, is high in efficiency, and does not affect the morphology of tissue cells and IHC staining while achieving depigmentation, and is suitable for depigmentation of melanin cells of melanoma tissue by using a full-automatic immunohistochemical staining instrument, and can realize automatic depigmentation.

The invention discloses a preparation method of bionic vesicles simultaneously targeting skin keratinocytes and fibroblasts and anti-aging application of the bionic vesicles, and belongs to the technical field of pharmaceutical preparations and cosmetics. According to the invention, the surface of liposome is modified with melanin cell membrane protein by using a microfluidic technology, and the membrane characteristics of melanin cells are integrated to a vesicle carrier through a biomimetic membrane structure, so that the effect of simultaneously targeting keratinocytes and fibroblasts is realized. The melanin cell membrane protein-derived bionic vesicles prepared by the invention can effectively target skin keratinocytes and fibroblasts, and the specific uptake of the vesicles by the cells is enhanced. After fisetin is loaded, the accumulation of fisetin in skin keratinocytes and fibroblasts can be remarkably improved, so that senescent cells are more effectively removed. Therefore, the bionic vesicles simultaneously targeting the skin keratinocytes and the fibroblasts, which are constructed by the invention, have great application prospects in the field of skin aging treatment.

The invention relates to the field of fluorescent probes and biological detection, and discloses a tyrosinase and hydrogen sulfide double-target fluorescent probe as well as a preparation method and application thereof. The fluorescent probe provided by the invention is characterized in that the fluorescence of the fluorescent probe is extremely weak, but when tyrosinase and hydrogen sulfide coexist, a specific chemical reaction can occur, the fluorescence effect is obviously started, and strong green fluorescence is generated at 517nm, so that the detection on the activity of tyrosinase and the content of hydrogen sulfide is realized. The probe not only can be used for detecting content changes of tyrosinase and hydrogen sulfide in human primary melanocytes, but also can be used for imaging analysis of human melanoma tissue slices, and a new tool is provided for early diagnosis and histological research of melanoma.

The application belongs to the technical field of biomedicine, and specifically discloses a gene marker of melanoma and application thereof. The gene marker is a PDP1 gene, and the application proves the correlation between the PDP1 gene and melanoma. The expression amount of the PDP1 gene in melanoma cells is significantly higher than that in normal melanocytes. The PDP1 expression is positively correlated with the pathological stage and the lymph node metastasis condition N value. The PDP1 gene influences the cell proliferation, cell apoptosis, cell migration and cell invasion of melanocytes. Therefore, the PDP1 gene can be used as a potential target for diagnosing and treating melanoma. By detecting the mRNA or protein expression level of the PDP1 gene, melanoma can be detected, and the application has good application value.

The invention discloses a method for analyzing the effect of active pharmaceutical ingredients between T cells and melanocytes in vitiligo. The method comprises the following steps: obtaining transcriptome data; performing differential gene analysis on the transcriptome data to obtain an analysis result; identifying a first gene associated with a disease; determining a second gene by using a machine learning algorithm; drawing a spatial distribution mode of a second gene in the leucoderma skin; carrying out molecular docking on the HSPA1A protein receptor and a small molecule drug ligand, and simulating and predicting a bioactive component for regulating a second gene in the drug; and evaluating the regulation effect of the bioactive components of the drug on the second gene expression pattern, endoplasmic reticulum stress and CD8 + T lymphocyte infiltration by using the animal model, and analyzing the effect of the active components of the drug between T cells and melanocytes. The method provided by the invention can be used for evaluating the effect of the active pharmaceutical ingredient in vitiligo treatment, and particularly determining the effect of the active pharmaceutical ingredient between T cells and melanin cells in vitiligo.

The application belongs to the technical field of cell induction differentiation culture and cell culture medium preparation, and particularly relates to a cell culture medium and application thereof in inducing olfactory mucosa mesenchymal stromal cells to differentiate into melanocytes. The culture medium has clear components, and the components used are mature commercial components, which are convenient and easy to obtain. The olfactory mucosa mesenchymal stromal cells are induced to become melanocytes expressing tyrosinase and containing melanin granules, which has the advantages of simple induction method and autologous induction avoiding ethical problems. The olfactory mucosa mesenchymal stromal cells are separated from nasal mucosa tissue, the nasal mucosa tissue is convenient to obtain, the nasal cavity part has rich blood supply, the wound is small and heals rapidly, and the human body is not continuously damaged. The prepared melanocytes can be used as seed cells for treating vitiligo disease, and have wide prospects.

The invention belongs to the field of recombinant proteins. The invention provides recombinant human-derived XVII type collagen, a composition with the effects of growing hair, growing hair, preventing hair loss, fixing hair and turning white hair into black hair and application of the recombinant human-derived XVII type collagen, the amino acid sequence of the recombinant human-derived XVII type collagen is shown as SEG ID NO. 1, and the invention further provides the composition with the effects of growing hair, growing hair, preventing hair loss, fixing hair and turning white hair into black hair. The recombinant human XVII type collagen has remarkable effects of promoting melanogenesis and promoting hair growth, and tests show that the recombinant human XVII type collagen has a remarkable effect of promoting the activity of HaCat cells and HFDPC cells; the composition with the effects of growing hair, nourishing hair, preventing hair loss, fixing hair and turning white hair into black hair has the effect of strongly promoting melanocyte secretion, and the hair growing effect is obvious.

The invention relates to application of Ro5-4864 or pharmaceutically acceptable salt in preparation of a medicine for treating and / or preventing melanocyte degeneration diseases caused by inflammatory immune response induced by oxidative stress. The melanocyte degeneration diseases caused by inflammatory immune response induced by oxidative stress comprise vitiligo, leukoplakia and pityriasis albicans. The Ro5-4864 and the pharmaceutically acceptable salt of the Ro5-4864, disclosed by the invention, inhibit continuous loss of melanocytes from oxidative stress, are strong in targeting effect and relatively small in side effect, and have remarkable advantages and progress compared with existing medicines.

The peptides of the invention have a sequence with at least 85% identity with a sequence SEQ ID NO: 1 or 2.These peptides regulate melanocyte activity in terms of proliferation and production of melanin. Therefore, they are useful in the treatment of melanomas but also of cosmetic cutaneous hyper or hypopigmentation.

This invention belongs to the field of cosmetic technology, specifically relating to a composition with water-locking and brightening functions and its targeted microcarrier delivery system. The water-locking and brightening composition provided by this invention mainly consists of niacinamide, tranexamic acid, oat β-glucan, disodium EDTA, and sodium hyaluronate. This composition can achieve a synergistic effect of water locking and brightening, effectively improving dullness, dryness, and uneven skin tone. Simultaneously, this invention also provides a targeted microcarrier delivery system, which is prepared by encapsulating the above composition in an internal aqueous phase using liposome technology, and simultaneously covalently attaching a targeting ligand to the surface of the liposomes. The targeted microcarrier delivery system obtained by this invention can actively target melanocytes and keratinocytes, achieving precise delivery of brightening and water-locking ingredients, significantly improving skin tone and addressing the root causes of dullness and uneven skin tone.

The invention provides a skin grafting device which comprises a skin taking structure and a driving part, the skin taking structure comprises a plurality of skin taking parts, each skin taking part comprises a tool bit and a first connecting part, the first connecting parts extend in the first direction, and the tool bits are connected to the ends, in the first direction, of the first connecting parts; the other end, in the first direction, of the first connecting part is rotatably connected to the driving part, the multiple skin taking parts are arranged at intervals in the direction perpendicular to the first direction, and the driving part is used for driving the skin taking parts to rotate around the axis parallel to the first direction. The multiple skin taking parts take skin at the same time, the skin taking efficiency is remarkably improved, in the rotation process of the skin taking parts, the tool bit extracts skin tissue, tiny autologous skin extracted by the skin taking parts carries hair follicles, sweat glands, melanocytes and the like, and the multiple skin taking parts carry the autologous skin to be implanted into allogeneic skin or artificial skin at the same time, so that a complete skin function reconstruction basis is provided, and the skin taking efficiency is improved. Therefore, the skin transplantation effect and the skin transplantation efficiency are improved, and meanwhile, a smaller wound is generated on a skin tissue donor region.

The application discloses a traditional Chinese medicine for treating scars, which comprises the following components in terms of weight: white chrysanthemum 10-15 parts, canty caterpillar fungus 8-12 parts, clove 20-30 parts, peach kernel 4-8 parts, soapberry 3-5 parts, dandelion 20-40 parts, schizonepeta 6-8 parts, salvia miltiorrhiza 8-12 parts, rhubarb 3-5 parts and licorice 6-10 parts. The application can obviously improve superficial scars and hypertrophic scars within one month, soften scar tissues, promote epithelialization of scar tissues and normalization of skin gland tissue secretion, disperse local melanocyte aggregation, and increase skin gloss and skin elasticity.

The present application belongs to the technical field of cosmetics, and particularly relates to a recombinant humanized collagen composition, a preparation method and application. The present application first reports a recombinant humanized collagen composition, which is based on the independently developed recombinant humanized collagen, and through compounding chitosan, ectoine and other components, the application scene of the recombinant type IV collagen is effectively expanded; and the composition itself can reduce the ability of melanocytes to synthesize melanin, has the ability to scavenge free radicals, and can also reduce the amount of preservatives in the composition, effectively maintain the stability of the recombinant type IV collagen. The recombinant humanized collagen composition can be used for daily skin care, and can also help the recovery after medical and aesthetic surgery, and therefore has good practical application value.

The invention provides application of a fibroblast growth factor 5 (FGF5) in promoting hair blackening, and relates to the technical field of biological medicines. In the invention, the recombinant FGF5 is prepared into a 200-500 ng / mL preparation for subcutaneous injection. After the FGF5 is combined with a receptor FGFR1 expressed on hair matrix cells, hair follicle melanocytes can be activated, melanin synthesis can be improved, melanocytes can be promoted to migrate to a hair matrix, and more melanin can be generated through differentiation to color hair. According to the invention, a new mechanism that the FGF5 and the receptor FGFR1 are combined to promote the migration of melanocytes is found for the first time, and the preparation containing the recombinant FGF5 is prepared according to the mechanism, so that a new way for promoting hair blackening is provided; a new thought is provided for the existing medicine for treating the leukotrichia.

The present application relates to the technical field of cell induced differentiation culture and cell culture medium preparation, and provides a cell culture medium and a use thereof in inducing the directional differentiation of olfactory mucosa mesenchymal stromal cells into melanocytes. The culture medium has a clear composition, and the components used are all conveniently and readily available mature commercial components. The culture medium can be used to induce olfactory mucosa mesenchymal stromal cells into melanocytes expressing tyrosinase and containing melanin particles, and has the advantages of a simple induction method and autoinduction to avoid ethical problems. In addition, the olfactory mucosa mesenchymal stromal cells are isolated from the nasal mucosa tissue, the nasal mucosa tissue is conveniently available, the nasal region has an abundant blood supply, and the wound is small and heals rapidly without causing continuous damage to the human body. The prepared melanocytes can be used as seed cells for treating vitiligo diseases and have wide prospects.

The present application relates to key miRNA and its application in regulating Liancheng white duck melanin production, the miRNA is miR-290, the nucleotide sequence is: 5'-CGGGAGCGGAGCUCGGCC-3'(SEQ ID NO:1), the application of the key miRNA in regulating Liancheng Liancheng white duck melanin production, the protein expression of ILK, JAK1, GSK3β, MC1R, TRP1 and TRP2 is targeted and regulated by the miR-290 through PI3K / Akt signal path, and it is applied in the selection of Liancheng white duck melanin character, such as the selection of Liancheng white duck black melanin character such as black beak, green foot, etc.The present application screens the key miRNA of difference as the control factor of research melanocyte proliferation or apoptosis, studies the effect of key miRNA on duck melanocyte and the regulation mode of target gene, and screens the meaningful candidate target gene.

The invention relates to a pharmaceutical composition with whitening, freckle removing and anti-aging functions and a preparation method of the pharmaceutical composition. Most of existing traditional Chinese medicine preparations such as lonicera hypoglauca flowers, humifuse euphorbia herbs, lysimachia christinae hance and sargentgloryvine stems pay attention to the effects of clearing away heat and toxic materials, promoting blood circulation to subdue swelling and the like. Nano micro-capsule powder prepared through different technological processes is matched with grape seeds and soybean extracts which are remarkable in whitening and anti-oxidation effect and extremely high in flavone content, and vitamin E derived from natural plants is matched, so that the effects of resisting oxidation, blocking tyrosine-tyrosinase series biochemical reactions in skin, inhibiting formation of melanin particles in the skin, improving the skin whitening effect and the skin whitening effect can be achieved, and the skin whitening effect is achieved. The whitening and freckle-removing composition has the advantages that melanin particles are prevented from ascending to epidermal cells to cuticle, melanin is effectively inhibited from being transmitted to keratinocytes, and melanin-containing cells are promoted to fall off, diminish inflammation, remove swelling and fade stains and wrinkles by accelerating the updating speed of skin cells, so that the whitening and freckle-removing effects are finally achieved.

The invention belongs to the field of skin medicine, and discloses application of camellia glycoside B in preparation of anti-aging products. The camellia glycoside B has a remarkable anti-aging effect on skin cells damaged by ultraviolet rays, and has remarkable effects of inhibiting generation of active oxygen and activating autophagy on the skin cells damaged by the ultraviolet rays. The invention discloses an application of camellia glycoside B in preparation of a whitening product. Camellia glycoside B can reduce melanogenesis and inhibit tyrosinase activity, can inhibit melanosome from being transferred from melanocytes to cuticle cells, and has a whitening effect.

This invention relates to a method for preparing a composition of loaded active peptide vesicles and its application in skincare products. The composition utilizes an ultrasound-assisted aqueous two-phase extraction system to extract active peptides from *Bombyx mori*, and then encapsulates them with natural plant vesicles of sage and bergamot to form a complex. It can penetrate the epidermal layer of the skin, targeting fibroblasts and melanocytes, exhibiting excellent antioxidant properties and the ability to scavenge a large number of free radicals. It inhibits the activity of matrix metalloproteinases, thereby reducing collagen loss; inhibits tyrosinase activity and reduces melanin production; and inhibits the secretion of pro-inflammatory factors, thus maintaining a stable and balanced state of the skin. This composition can delay skin aging, brighten skin tone, and soothe inflammation.