54 results about "Melanophore" patented technology

The invention relates to a phytoextraction external liniment for promoting black hair growth and a preparation method thereof. According to the liniment, a polygonum multiflorum extract, a cacumen biotae supercritical extract and molecular embedded black sesamin in a specific proportion are taken as cores and are matched with an azone-menthol transdermal enhancer and a propylene glycol-olive oil carrier, and black hair growth is synergistically promoted through triple mechanisms of activating tyrosinase, inhibiting melanocyte apoptosis and promoting cell migration. The preparation method adopts a gradient temperature extraction combined molecular embedding technology, the polygonum multiflorum is subjected to stepped purification at 40-60 DEG C-80 DEG C to retain thermosensitive active ingredients, the black sesamin is embedded by beta-cyclodextrin to improve the stability, and finally, the nano microemulsion is formed through two-stage homogeneous emulsification. The liniment can efficiently permeate into the deep layer of hair follicles, the efficiency of turning white hair into black is remarkably improved, and skin irritation is avoided.

The invention discloses a polypeptide-nucleic acid tetrahedron compound as well as a preparation method and application thereof. The compound comprises a nucleic acid tetrahedron and polypeptide, wherein the polypeptide is nonapeptide-1 with an amino acid sequence as shown in SEQ ID NO: 13; the nucleic acid tetrahedron is formed by self-assembly of four single-stranded DNAs with the same length. According to the compound, the transdermal function of nucleic acid tetrahedron capable of inhibiting melanogenesis and the melanocyte targeting function of nonapeptide-1 are combined, so that the TDN-NA-1 compound has the melanocyte targeting property while having the autonomous transdermal ability, and the blackness inhibiting effects of the nucleic acid tetrahedron and the nonapeptide-1 are combined, so that the effect of inhibiting melanogenesis is achieved. Therefore, the whitening composition has a stronger capability of inhibiting melanin generation, so that the purpose of precise whitening is achieved, and the whitening composition has a wide application prospect in the field of cosmetics.

The invention discloses a melanocyte dissolution and separation device and a use method. The melanocyte dissolution and separation device comprises a case and a lifting assembly, a central shaft is rotationally connected in the case, a placement groove is formed in the upper end of the central shaft, supporting seats which are uniformly distributed are arranged at the upper end of the outer arc surface of the central shaft, rotating shafts are rotationally connected to the ends, away from the central shaft, of the multiple supporting seats, test tube seats are arranged outside the multiple rotating shafts, and adjustable tube seat assemblies are movably clamped to the bottoms of the test tube seats; according to the melanocyte dissolution and separation device and the use method, the test tube seat is provided with the adjustable base, so that test tubes with different lengths can be conveniently placed, the test tubes can be automatically clamped after being inserted, the angle of the test tube group is adjustable, the use flexibility is good, and the test tube group is convenient to use and high in practicability. Supernate in a plurality of test tubes can be discharged synchronously, and the use convenience is good.

The invention discloses a method for regulating the body color of ornamental fish, and belongs to the technical field of aquaculture. According to the method disclosed by the invention, the aquarium fishes in the fry stage and the juvenile stage are soaked with thyroid hormone, so that the excessive thyroid hormone can obviously reduce the number of haematochrome cells and the size of pigment particles of the aquarium fishes in different stages, and reduce the content of carotenoid; when the synthesis of thyroid hormone is inhibited, gene expression of melanocytes and related synthetic pathways can be promoted, and meanwhile, the carotenoid level is increased. The inhibition effect of thyroid hormone on scatophagus argus haematochrome cell development and carotenoid accumulation is determined for the first time, the knowledge blank in the field is filled, and important guidance information is provided for body color regulation and genetic improvement of aquarium fish and other aquatic animals in the future.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention discloses a whitening composition as well as a preparation method and application thereof. The whitening composition is prepared from the following components: a tea leaf extract, a lavender flower extract and water, wherein the tea leaf extract, the lavender flower extract and the water are added into the whitening composition. The whitening composition can interact and synergistically improve the autophagy ability of cells, synergistically improve the phagocytosis of melanin in keratinocytes and the removal ability of skin metabolic wastes, can synergistically inhibit the generation of melanin and synergistically improve the expression of miR-137 genes in melanocytes, has a relatively high development prospect of cosmetics with whitening effects, and has a wide application prospect. Moreover, the composition is mild in property, easy to absorb and non-irritant, and can be widely applied to skin care cosmetics.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention provides siRNA lipid nanoparticles for targeted therapy of chloasma as well as a preparation method and application of the siRNA lipid nanoparticles, and belongs to the technical field of biological medicines. The invention provides LNP-siRNAs for treating chloasma. The LNP-siRNAs for treating chloasma comprise siRNAs of key molecules for targeted inhibition of melanin formation. According to the invention, an effective siRNA sequence is screened out and verified in cells, then siRNAs with an obvious inhibition effect are packaged into LNP-siRNAs, the packaging quality of the LNP-siRNAs is evaluated, and the LNP-siRNAs are applied to cells and animals to evaluate the inhibition effect of the LNP-siRNAs on melanin. The packaged LNP-siRNAs are used for melanocytes and animal skin, can effectively inhibit the formation of melanin, and can be used for screening and preparing potential medicines for clinically treating chloasma.

This invention discloses a skin-whitening composition that reduces aging melanocytes and its application, relating to the cosmetics field. The invention provides a composition that, by reducing aging melanocytes and decreasing SASP secretion, reduces melanin production and inhibits tyrosinase activity while maintaining healthy melanocytes, thus maintaining skin homeostasis while whitening the skin. This composition can be widely used in various skincare products to effectively achieve long-lasting whitening effects.

The application discloses a method for regulating body color of ornamental fish and belongs to the technical field of aquaculture. The application finds that, by using thyroid hormone to soak ornamental fish in the fry period and the juvenile fish period, excessive thyroid hormone can significantly reduce the number of red pigment cells and the size of pigment granules of ornamental fish in different periods, and reduce the content of carotenoids; when the synthesis of thyroid hormone is inhibited, the expression of melanin cells and related synthesis pathway genes is promoted, and the carotenoid level is increased. The application first clearly defines the inhibitory effect of thyroid hormone on the development of red pigment cells and the accumulation of carotenoids of gourami, fills the knowledge gap in the field, and provides important guiding information for future regulation of body color and genetic improvement of ornamental fish and other aquatic animals.

The invention relates to the related technical field of cell culture, in particular to a melanocyte cultivation device and method.The melanocyte cultivation device comprises a cabinet body, a pushing mechanism is arranged on the inner side surface of the cabinet body, and a pushing-back mechanism is arranged on the surface of one side of the pushing mechanism. The pushing mechanism and the pushing-back mechanism are matched with each other, so that cells are observed more conveniently, the risks of environment instability and cell pollution caused by frequent opening of the incubator are reduced, the operation process is simplified, an operator only needs to set parameters and start corresponding programs on a display control panel, and the operation efficiency is improved. A stable and suitable growth environment can be provided for melanocytes, the cell growth and proliferation efficiency can be improved, the cell activity and quality are guaranteed, an operator can conveniently obtain culture dishes of different layers through the lifting function of the pushing mechanism, the requirements for observing and operating cells in different culture stages at the same time are met, and the culture efficiency is improved. And the flexibility and the efficiency of experimental operation are greatly improved.

The invention relates to the field of cosmetics, in particular to a compound and application thereof. The inventor creatively discovers that the compound with the structure shown in the formula I has the effect of inhibiting melanocytes and tyrosinase, and proves that the compound has new application in whitening and has application in preparation of whitening products. Experiments show. The compound with the structure shown in the formula I is superior to the traditional capsaicin with a whitening effect in the inhibition effect on melanocytes and tyrosinase.

The present specification relates to a composition capable of preventing or ameliorating skin aging by adjusting the aging index of melanocytes. The composition according to the present disclosure contains a thymol ester-based compound as an active ingredient, thereby being capable of exhibiting an effect of preventing or improving skin aging when applied to a subject in which skin cells become flat or large or beta-galactosidase of melanocytes is activated.

The purpose of the present invention is to suppress the effect of ultraviolet light on melanocytes. Upon performance of gene expression analysis with a focus on the olfactory receptor OR2L13 expressed by melanocytes, it was discovered that addition of (−)-carvone, a ligand of OR2L13, increased the expression of genes related to suppression of active oxygen, suppression of the production of inflammatory cytokines or chemokines, suppression of senescence, and / or degradation of abnormal proteins. As a result of said discovery, the present invention provides an inhibitor of the effects of ultraviolet light on melanocytes that includes (−)-carvone.

The application discloses a white hair to black hair essence and a preparation method thereof. The white hair to black hair essence is prepared from selected raw materials, such as water dropwort leaf / stem extract, oxyhematin, radix polygoni multiflori, panax notoginseng and the like, and has good effects on activating hair follicles, enhancing the activity of melanocytes in the hair follicles and promoting melanin production. Volunteer tests prove that with the growth of new black hair and the shedding of aging white hair, the black hair gradually increases and the white hair gradually decreases by long-term use of the white hair to black hair essence, and the sensory effect of 'white hair to black hair' is achieved.

Provided is a method for obtaining the germ cell lineage of an oviparous vertebrate more efficiently than conventional techniques. A host oviparous vertebrate is prepared at a developmental stage after the development of black pigmentary cells in the retina and before the formation of multiple layers of germ cells in the genitals, and isolated undifferentiated germ cells from a donor oviparous vertebrate are transplanted into the host oviparous vertebrate.

The invention provides a preparation method, a product and application of a Grass rose extract, and belongs to the technical field of cosmetic raw materials. The method comprises the following steps: mixing Grass rose with a solvent, and carrying out flash extraction to obtain a Grass rose extract; wherein the solvent is a butanediol aqueous solution. The content of astragalus smicus glycoside, ellagic acid and isoquercitrin in the Grass rose extract prepared by adopting the preparation method is higher, the proportion of astragalus smicus glycoside, ellagic acid and isoquercitrin is more beneficial to reducing the content of melanin in melanocytes and the activity of tyrosinase, and a basis is provided for application of the Grass rose extract in whitening cosmetics.

The invention relates to the technical field of cosmetics, in particular to a mark fading and repairing composition as well as a preparation method and application thereof. The invention relates to a mark fading and repairing composition which comprises the following components in percentage by weight: 0.1%-5% of dragon's blood extract, 0.01%-0.5% of keratinase and 1%-5% of nicotinamide, the preparation method comprises the following steps: preparing the eutectic solvent; performing ultrasonic-assisted extraction; separation and post-treatment; the invention has the following beneficial effects: the resina draconis extract can inhibit continuous pigmentation caused by acne inflammation residue, promote healing of skin minimal wounds and reduce dermis layer injury of acne mark parts; keratinase specifically hydrolyzes stratum corneum aged keratin, cutin metabolism of acne mark parts is accelerated, and accumulation of pigments in stratum corneum is avoided; nicotinamide inhibits melanocytes from transferring melanin to keratinocytes; through the synergistic effect of the three components, a closed-loop printing fading mechanism of anti-inflammatory repair-keratin metabolism-pigment inhibition is formed, the printing fading effect is more comprehensive and more efficient, and skin repair and barrier protection can be both considered.

This invention discloses a feature selection method and system based on an improved horned lizard optimization algorithm, relating to the technical field of data feature selection. The method comprises: constructing an improved horned lizard optimization algorithm (bROBEHLOA) using the α-melanophore (MSH) rate, a biogeographic learning strategy (BLS), a hierarchical structure (HS), a horned lizard optimization algorithm (HLOA), general backward learning (OBL), and a random follow-up (RF) algorithm to update individual positions; initializing the algorithm population based on a medical dataset, using a KNN (Kernest Neighbor) model to obtain the fitness value of each individual in the population, and using the bROBEHLOA algorithm to find the optimal feature subset. The optimal feature subset is output when the maximum number of iterations is reached. This method can prevent the algorithm from falling into a local optimal solution, which may lead to the omission of key features.

The invention relates to a method for separating melanocytes from human hair follicles in vitro and application. The method comprises the following steps: 1) separating hair follicle front end tissues from hair follicle tissues obtained from an in-vitro human hair sample; (2) treating the hair follicle front-end tissue obtained in the step (1) by using digestive enzyme; and (3) culturing the hair follicle front-end tissue treated in the step (2) by adopting a melanocyte culture medium. The invention also provides application of the melanocytes from human hair follicles in preparation of products for melanin deficiency diseases, poliosis and the like, and provides data support in the aspects of skin physiology, pathology, pharmacology, pharmacotoxicology, tissue engineering, gene therapy and the like. According to the method, primary separation of the melanocytes is carried out by taking the hair follicle tissue as a source, the purity of the cells is effectively improved through pretreatment, the operation difficulty of obtaining the pure melanocytes through separation is reduced, and more feasibility is provided for preparing products for melanin deficiency diseases, poliosis and the like.

According to the whitening, anti-aging and anti-oxidation composition containing the phalaenopsis amabilis extract and the preparation method and application of the whitening, anti-aging and anti-oxidation composition, the phalaenopsis amabilis extract, the bryophyllum pinnatum leaf extract and the liquorice extract are combined, and the macroporous adsorption resin with the specific pore diameter is adopted for purification treatment, so that the purity of active components is improved, and the anti-aging and anti-oxidation effects are improved; interference of harmful substances on subsequent multi-stage active screening is avoided; through multi-stage active screening after purification, flavonoid substances with specific molecular weight structures are contained in the phalaenopsis amabilis extract, and the flavonoid substances, the air plant leaf extract and the liquorice extract are synergistic, so that the inhibition capability on tyrosinase activity is greatly enhanced, and the melanin generation capability of melanin cells is remarkably inhibited; therefore, the overall whitening, anti-aging and anti-oxidation effects are improved; the composition can play a synergistic whitening, anti-aging and anti-oxidation role, and has excellent whitening, anti-aging and anti-oxidation activity. The adopted raw materials are free of excessive additives, mainly comprise plant extracts, and are free of side effects and mild in formula.

The application discloses a method suitable for depigmentation of melanoma tissue in immunohistochemical staining, and the method comprises the following steps: after an antigen repairing step in the immunohistochemical staining process, a depigmentation operation is performed; a hydrogen peroxide methanol solution is used as a depigmentation solution; and the depigmentation is performed through heating incubation, wherein the heating temperature is 55 DEG C to 75 DEG C, the volume fraction of the hydrogen peroxide methanol solution is 5% to 15%, and the incubation time is 10 to 20 minutes. The method simplifies the depigmentation process of melanin, is high in efficiency, and does not affect the morphology of tissue cells and IHC staining while achieving depigmentation, and is suitable for depigmentation of melanin cells of melanoma tissue by using a full-automatic immunohistochemical staining instrument, and can realize automatic depigmentation.

The invention discloses a preparation method of bionic vesicles simultaneously targeting skin keratinocytes and fibroblasts and anti-aging application of the bionic vesicles, and belongs to the technical field of pharmaceutical preparations and cosmetics. According to the invention, the surface of liposome is modified with melanin cell membrane protein by using a microfluidic technology, and the membrane characteristics of melanin cells are integrated to a vesicle carrier through a biomimetic membrane structure, so that the effect of simultaneously targeting keratinocytes and fibroblasts is realized. The melanin cell membrane protein-derived bionic vesicles prepared by the invention can effectively target skin keratinocytes and fibroblasts, and the specific uptake of the vesicles by the cells is enhanced. After fisetin is loaded, the accumulation of fisetin in skin keratinocytes and fibroblasts can be remarkably improved, so that senescent cells are more effectively removed. Therefore, the bionic vesicles simultaneously targeting the skin keratinocytes and the fibroblasts, which are constructed by the invention, have great application prospects in the field of skin aging treatment.

The invention relates to the field of fluorescent probes and biological detection, and discloses a tyrosinase and hydrogen sulfide double-target fluorescent probe as well as a preparation method and application thereof. The fluorescent probe provided by the invention is characterized in that the fluorescence of the fluorescent probe is extremely weak, but when tyrosinase and hydrogen sulfide coexist, a specific chemical reaction can occur, the fluorescence effect is obviously started, and strong green fluorescence is generated at 517nm, so that the detection on the activity of tyrosinase and the content of hydrogen sulfide is realized. The probe not only can be used for detecting content changes of tyrosinase and hydrogen sulfide in human primary melanocytes, but also can be used for imaging analysis of human melanoma tissue slices, and a new tool is provided for early diagnosis and histological research of melanoma.

The application belongs to the technical field of biomedicine, and specifically discloses a gene marker of melanoma and application thereof. The gene marker is a PDP1 gene, and the application proves the correlation between the PDP1 gene and melanoma. The expression amount of the PDP1 gene in melanoma cells is significantly higher than that in normal melanocytes. The PDP1 expression is positively correlated with the pathological stage and the lymph node metastasis condition N value. The PDP1 gene influences the cell proliferation, cell apoptosis, cell migration and cell invasion of melanocytes. Therefore, the PDP1 gene can be used as a potential target for diagnosing and treating melanoma. By detecting the mRNA or protein expression level of the PDP1 gene, melanoma can be detected, and the application has good application value.

The present invention relates to a composition for skin whitening and a method for preparing same and, to a composition for skin whitening and a method for preparing same, in which a compound having a specific molecular structure and derived from a culture of Streptomyces sp. strains is used to inhibit the melanin production of melanoma cells and improve skin whitening effects.

The invention discloses a method for analyzing the effect of active pharmaceutical ingredients between T cells and melanocytes in vitiligo. The method comprises the following steps: obtaining transcriptome data; performing differential gene analysis on the transcriptome data to obtain an analysis result; identifying a first gene associated with a disease; determining a second gene by using a machine learning algorithm; drawing a spatial distribution mode of a second gene in the leucoderma skin; carrying out molecular docking on the HSPA1A protein receptor and a small molecule drug ligand, and simulating and predicting a bioactive component for regulating a second gene in the drug; and evaluating the regulation effect of the bioactive components of the drug on the second gene expression pattern, endoplasmic reticulum stress and CD8 + T lymphocyte infiltration by using the animal model, and analyzing the effect of the active components of the drug between T cells and melanocytes. The method provided by the invention can be used for evaluating the effect of the active pharmaceutical ingredient in vitiligo treatment, and particularly determining the effect of the active pharmaceutical ingredient between T cells and melanin cells in vitiligo.

The application belongs to the technical field of cell induction differentiation culture and cell culture medium preparation, and particularly relates to a cell culture medium and application thereof in inducing olfactory mucosa mesenchymal stromal cells to differentiate into melanocytes. The culture medium has clear components, and the components used are mature commercial components, which are convenient and easy to obtain. The olfactory mucosa mesenchymal stromal cells are induced to become melanocytes expressing tyrosinase and containing melanin granules, which has the advantages of simple induction method and autologous induction avoiding ethical problems. The olfactory mucosa mesenchymal stromal cells are separated from nasal mucosa tissue, the nasal mucosa tissue is convenient to obtain, the nasal cavity part has rich blood supply, the wound is small and heals rapidly, and the human body is not continuously damaged. The prepared melanocytes can be used as seed cells for treating vitiligo disease, and have wide prospects.

The invention belongs to but not limited to the technical field of gene engineering, and particularly relates to a method for constructing blue and white porcelain blue body color fancy carp through a pigment regulation gene tyr, which comprises the following steps: constructing a transgenic vector which is expressed by blue protein driven by a fancy carp tyr promoter, preparing Tol2 transposase mRNA (messenger Ribonucleic Acid), selecting and matching parent fish, collecting fertilized eggs and performing debonding treatment. In the early fertilization stage, the carrier and mRNA are guided into fertilized eggs through microinjection, incubation culture is conducted, and the transgenic juvenile fish specifically expressing blue protein in the melanocyte distribution area is obtained. Aiming at the injection difficulties that the fertilized eggs of the fancy carp absorb water to swell, egg membranes become hard and the like, the debonding and injection operation conditions are optimized, and the injection success rate and the embryo survival rate are effectively improved. According to the method, the spatial expression control of the blue color blocks of the fancy carp is realized, a visual means is provided for researching the body color formation mechanism of the fancy carp, meanwhile, technical support is provided for germplasm innovation and commercial development of featured strains of the fancy carp, and the method has relatively high scientific research and application values.

The present invention belongs to the field of cosmetics technology and provides a traditional Chinese medicine composition for blackening and growing hair, as well as its preparation method and application. The traditional Chinese medicine composition comprises the following raw materials by weight: 3-6 parts of Polygonum multiflorum, 3-6 parts of Angelica sinensis, 2-5 parts of Oroxylum indicum, and 3-5 parts of Lycium barbarum. The composition has the effect of alleviating the growth environment of hair follicles and promoting the production of melanin by melanocytes. Furthermore, the composition utilizes nanotechnology to facilitate scalp absorption. The preparation method of the traditional Chinese medicine composition of the present invention is simple and convenient, and is conducive to large-scale production and application.