42 results about "Melanophore" patented technology

The invention relates to a phytoextraction external liniment for promoting black hair growth and a preparation method thereof. According to the liniment, a polygonum multiflorum extract, a cacumen biotae supercritical extract and molecular embedded black sesamin in a specific proportion are taken as cores and are matched with an azone-menthol transdermal enhancer and a propylene glycol-olive oil carrier, and black hair growth is synergistically promoted through triple mechanisms of activating tyrosinase, inhibiting melanocyte apoptosis and promoting cell migration. The preparation method adopts a gradient temperature extraction combined molecular embedding technology, the polygonum multiflorum is subjected to stepped purification at 40-60 DEG C-80 DEG C to retain thermosensitive active ingredients, the black sesamin is embedded by beta-cyclodextrin to improve the stability, and finally, the nano microemulsion is formed through two-stage homogeneous emulsification. The liniment can efficiently permeate into the deep layer of hair follicles, the efficiency of turning white hair into black is remarkably improved, and skin irritation is avoided.

The invention discloses a polypeptide-nucleic acid tetrahedron compound as well as a preparation method and application thereof. The compound comprises a nucleic acid tetrahedron and polypeptide, wherein the polypeptide is nonapeptide-1 with an amino acid sequence as shown in SEQ ID NO: 13; the nucleic acid tetrahedron is formed by self-assembly of four single-stranded DNAs with the same length. According to the compound, the transdermal function of nucleic acid tetrahedron capable of inhibiting melanogenesis and the melanocyte targeting function of nonapeptide-1 are combined, so that the TDN-NA-1 compound has the melanocyte targeting property while having the autonomous transdermal ability, and the blackness inhibiting effects of the nucleic acid tetrahedron and the nonapeptide-1 are combined, so that the effect of inhibiting melanogenesis is achieved. Therefore, the whitening composition has a stronger capability of inhibiting melanin generation, so that the purpose of precise whitening is achieved, and the whitening composition has a wide application prospect in the field of cosmetics.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention discloses a whitening composition as well as a preparation method and application thereof. The whitening composition is prepared from the following components: a tea leaf extract, a lavender flower extract and water, wherein the tea leaf extract, the lavender flower extract and the water are added into the whitening composition. The whitening composition can interact and synergistically improve the autophagy ability of cells, synergistically improve the phagocytosis of melanin in keratinocytes and the removal ability of skin metabolic wastes, can synergistically inhibit the generation of melanin and synergistically improve the expression of miR-137 genes in melanocytes, has a relatively high development prospect of cosmetics with whitening effects, and has a wide application prospect. Moreover, the composition is mild in property, easy to absorb and non-irritant, and can be widely applied to skin care cosmetics.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

This invention discloses a skin-whitening composition that reduces aging melanocytes and its application, relating to the cosmetics field. The invention provides a composition that, by reducing aging melanocytes and decreasing SASP secretion, reduces melanin production and inhibits tyrosinase activity while maintaining healthy melanocytes, thus maintaining skin homeostasis while whitening the skin. This composition can be widely used in various skincare products to effectively achieve long-lasting whitening effects.

The application discloses a method for regulating body color of ornamental fish and belongs to the technical field of aquaculture. The application finds that, by using thyroid hormone to soak ornamental fish in the fry period and the juvenile fish period, excessive thyroid hormone can significantly reduce the number of red pigment cells and the size of pigment granules of ornamental fish in different periods, and reduce the content of carotenoids; when the synthesis of thyroid hormone is inhibited, the expression of melanin cells and related synthesis pathway genes is promoted, and the carotenoid level is increased. The application first clearly defines the inhibitory effect of thyroid hormone on the development of red pigment cells and the accumulation of carotenoids of gourami, fills the knowledge gap in the field, and provides important guiding information for future regulation of body color and genetic improvement of ornamental fish and other aquatic animals.

The invention relates to the related technical field of cell culture, in particular to a melanocyte cultivation device and method.The melanocyte cultivation device comprises a cabinet body, a pushing mechanism is arranged on the inner side surface of the cabinet body, and a pushing-back mechanism is arranged on the surface of one side of the pushing mechanism. The pushing mechanism and the pushing-back mechanism are matched with each other, so that cells are observed more conveniently, the risks of environment instability and cell pollution caused by frequent opening of the incubator are reduced, the operation process is simplified, an operator only needs to set parameters and start corresponding programs on a display control panel, and the operation efficiency is improved. A stable and suitable growth environment can be provided for melanocytes, the cell growth and proliferation efficiency can be improved, the cell activity and quality are guaranteed, an operator can conveniently obtain culture dishes of different layers through the lifting function of the pushing mechanism, the requirements for observing and operating cells in different culture stages at the same time are met, and the culture efficiency is improved. And the flexibility and the efficiency of experimental operation are greatly improved.

The invention relates to the field of cosmetics, in particular to a compound and application thereof. The inventor creatively discovers that the compound with the structure shown in the formula I has the effect of inhibiting melanocytes and tyrosinase, and proves that the compound has new application in whitening and has application in preparation of whitening products. Experiments show. The compound with the structure shown in the formula I is superior to the traditional capsaicin with a whitening effect in the inhibition effect on melanocytes and tyrosinase.

The purpose of the present invention is to suppress the effect of ultraviolet light on melanocytes. Upon performance of gene expression analysis with a focus on the olfactory receptor OR2L13 expressed by melanocytes, it was discovered that addition of (−)-carvone, a ligand of OR2L13, increased the expression of genes related to suppression of active oxygen, suppression of the production of inflammatory cytokines or chemokines, suppression of senescence, and / or degradation of abnormal proteins. As a result of said discovery, the present invention provides an inhibitor of the effects of ultraviolet light on melanocytes that includes (−)-carvone.

The application discloses a white hair to black hair essence and a preparation method thereof. The white hair to black hair essence is prepared from selected raw materials, such as water dropwort leaf / stem extract, oxyhematin, radix polygoni multiflori, panax notoginseng and the like, and has good effects on activating hair follicles, enhancing the activity of melanocytes in the hair follicles and promoting melanin production. Volunteer tests prove that with the growth of new black hair and the shedding of aging white hair, the black hair gradually increases and the white hair gradually decreases by long-term use of the white hair to black hair essence, and the sensory effect of 'white hair to black hair' is achieved.

Provided is a method for obtaining the germ cell lineage of an oviparous vertebrate more efficiently than conventional techniques. A host oviparous vertebrate is prepared at a developmental stage after the development of black pigmentary cells in the retina and before the formation of multiple layers of germ cells in the genitals, and isolated undifferentiated germ cells from a donor oviparous vertebrate are transplanted into the host oviparous vertebrate.

The invention relates to the technical field of cosmetics, in particular to a mark fading and repairing composition as well as a preparation method and application thereof. The invention relates to a mark fading and repairing composition which comprises the following components in percentage by weight: 0.1%-5% of dragon's blood extract, 0.01%-0.5% of keratinase and 1%-5% of nicotinamide, the preparation method comprises the following steps: preparing the eutectic solvent; performing ultrasonic-assisted extraction; separation and post-treatment; the invention has the following beneficial effects: the resina draconis extract can inhibit continuous pigmentation caused by acne inflammation residue, promote healing of skin minimal wounds and reduce dermis layer injury of acne mark parts; keratinase specifically hydrolyzes stratum corneum aged keratin, cutin metabolism of acne mark parts is accelerated, and accumulation of pigments in stratum corneum is avoided; nicotinamide inhibits melanocytes from transferring melanin to keratinocytes; through the synergistic effect of the three components, a closed-loop printing fading mechanism of anti-inflammatory repair-keratin metabolism-pigment inhibition is formed, the printing fading effect is more comprehensive and more efficient, and skin repair and barrier protection can be both considered.

This invention discloses a feature selection method and system based on an improved horned lizard optimization algorithm, relating to the technical field of data feature selection. The method comprises: constructing an improved horned lizard optimization algorithm (bROBEHLOA) using the α-melanophore (MSH) rate, a biogeographic learning strategy (BLS), a hierarchical structure (HS), a horned lizard optimization algorithm (HLOA), general backward learning (OBL), and a random follow-up (RF) algorithm to update individual positions; initializing the algorithm population based on a medical dataset, using a KNN (Kernest Neighbor) model to obtain the fitness value of each individual in the population, and using the bROBEHLOA algorithm to find the optimal feature subset. The optimal feature subset is output when the maximum number of iterations is reached. This method can prevent the algorithm from falling into a local optimal solution, which may lead to the omission of key features.

The invention relates to a method for separating melanocytes from human hair follicles in vitro and application. The method comprises the following steps: 1) separating hair follicle front end tissues from hair follicle tissues obtained from an in-vitro human hair sample; (2) treating the hair follicle front-end tissue obtained in the step (1) by using digestive enzyme; and (3) culturing the hair follicle front-end tissue treated in the step (2) by adopting a melanocyte culture medium. The invention also provides application of the melanocytes from human hair follicles in preparation of products for melanin deficiency diseases, poliosis and the like, and provides data support in the aspects of skin physiology, pathology, pharmacology, pharmacotoxicology, tissue engineering, gene therapy and the like. According to the method, primary separation of the melanocytes is carried out by taking the hair follicle tissue as a source, the purity of the cells is effectively improved through pretreatment, the operation difficulty of obtaining the pure melanocytes through separation is reduced, and more feasibility is provided for preparing products for melanin deficiency diseases, poliosis and the like.

The application discloses a method suitable for depigmentation of melanoma tissue in immunohistochemical staining, and the method comprises the following steps: after an antigen repairing step in the immunohistochemical staining process, a depigmentation operation is performed; a hydrogen peroxide methanol solution is used as a depigmentation solution; and the depigmentation is performed through heating incubation, wherein the heating temperature is 55 DEG C to 75 DEG C, the volume fraction of the hydrogen peroxide methanol solution is 5% to 15%, and the incubation time is 10 to 20 minutes. The method simplifies the depigmentation process of melanin, is high in efficiency, and does not affect the morphology of tissue cells and IHC staining while achieving depigmentation, and is suitable for depigmentation of melanin cells of melanoma tissue by using a full-automatic immunohistochemical staining instrument, and can realize automatic depigmentation.

The invention discloses a preparation method of bionic vesicles simultaneously targeting skin keratinocytes and fibroblasts and anti-aging application of the bionic vesicles, and belongs to the technical field of pharmaceutical preparations and cosmetics. According to the invention, the surface of liposome is modified with melanin cell membrane protein by using a microfluidic technology, and the membrane characteristics of melanin cells are integrated to a vesicle carrier through a biomimetic membrane structure, so that the effect of simultaneously targeting keratinocytes and fibroblasts is realized. The melanin cell membrane protein-derived bionic vesicles prepared by the invention can effectively target skin keratinocytes and fibroblasts, and the specific uptake of the vesicles by the cells is enhanced. After fisetin is loaded, the accumulation of fisetin in skin keratinocytes and fibroblasts can be remarkably improved, so that senescent cells are more effectively removed. Therefore, the bionic vesicles simultaneously targeting the skin keratinocytes and the fibroblasts, which are constructed by the invention, have great application prospects in the field of skin aging treatment.

The invention relates to the field of fluorescent probes and biological detection, and discloses a tyrosinase and hydrogen sulfide double-target fluorescent probe as well as a preparation method and application thereof. The fluorescent probe provided by the invention is characterized in that the fluorescence of the fluorescent probe is extremely weak, but when tyrosinase and hydrogen sulfide coexist, a specific chemical reaction can occur, the fluorescence effect is obviously started, and strong green fluorescence is generated at 517nm, so that the detection on the activity of tyrosinase and the content of hydrogen sulfide is realized. The probe not only can be used for detecting content changes of tyrosinase and hydrogen sulfide in human primary melanocytes, but also can be used for imaging analysis of human melanoma tissue slices, and a new tool is provided for early diagnosis and histological research of melanoma.

The application belongs to the technical field of biomedicine, and specifically discloses a gene marker of melanoma and application thereof. The gene marker is a PDP1 gene, and the application proves the correlation between the PDP1 gene and melanoma. The expression amount of the PDP1 gene in melanoma cells is significantly higher than that in normal melanocytes. The PDP1 expression is positively correlated with the pathological stage and the lymph node metastasis condition N value. The PDP1 gene influences the cell proliferation, cell apoptosis, cell migration and cell invasion of melanocytes. Therefore, the PDP1 gene can be used as a potential target for diagnosing and treating melanoma. By detecting the mRNA or protein expression level of the PDP1 gene, melanoma can be detected, and the application has good application value.

The invention discloses a method for analyzing the effect of active pharmaceutical ingredients between T cells and melanocytes in vitiligo. The method comprises the following steps: obtaining transcriptome data; performing differential gene analysis on the transcriptome data to obtain an analysis result; identifying a first gene associated with a disease; determining a second gene by using a machine learning algorithm; drawing a spatial distribution mode of a second gene in the leucoderma skin; carrying out molecular docking on the HSPA1A protein receptor and a small molecule drug ligand, and simulating and predicting a bioactive component for regulating a second gene in the drug; and evaluating the regulation effect of the bioactive components of the drug on the second gene expression pattern, endoplasmic reticulum stress and CD8 + T lymphocyte infiltration by using the animal model, and analyzing the effect of the active components of the drug between T cells and melanocytes. The method provided by the invention can be used for evaluating the effect of the active pharmaceutical ingredient in vitiligo treatment, and particularly determining the effect of the active pharmaceutical ingredient between T cells and melanin cells in vitiligo.

The application belongs to the technical field of cell induction differentiation culture and cell culture medium preparation, and particularly relates to a cell culture medium and application thereof in inducing olfactory mucosa mesenchymal stromal cells to differentiate into melanocytes. The culture medium has clear components, and the components used are mature commercial components, which are convenient and easy to obtain. The olfactory mucosa mesenchymal stromal cells are induced to become melanocytes expressing tyrosinase and containing melanin granules, which has the advantages of simple induction method and autologous induction avoiding ethical problems. The olfactory mucosa mesenchymal stromal cells are separated from nasal mucosa tissue, the nasal mucosa tissue is convenient to obtain, the nasal cavity part has rich blood supply, the wound is small and heals rapidly, and the human body is not continuously damaged. The prepared melanocytes can be used as seed cells for treating vitiligo disease, and have wide prospects.

The invention belongs to but not limited to the technical field of gene engineering, and particularly relates to a method for constructing blue and white porcelain blue body color fancy carp through a pigment regulation gene tyr, which comprises the following steps: constructing a transgenic vector which is expressed by blue protein driven by a fancy carp tyr promoter, preparing Tol2 transposase mRNA (messenger Ribonucleic Acid), selecting and matching parent fish, collecting fertilized eggs and performing debonding treatment. In the early fertilization stage, the carrier and mRNA are guided into fertilized eggs through microinjection, incubation culture is conducted, and the transgenic juvenile fish specifically expressing blue protein in the melanocyte distribution area is obtained. Aiming at the injection difficulties that the fertilized eggs of the fancy carp absorb water to swell, egg membranes become hard and the like, the debonding and injection operation conditions are optimized, and the injection success rate and the embryo survival rate are effectively improved. According to the method, the spatial expression control of the blue color blocks of the fancy carp is realized, a visual means is provided for researching the body color formation mechanism of the fancy carp, meanwhile, technical support is provided for germplasm innovation and commercial development of featured strains of the fancy carp, and the method has relatively high scientific research and application values.

The present invention belongs to the field of cosmetics technology and provides a traditional Chinese medicine composition for blackening and growing hair, as well as its preparation method and application. The traditional Chinese medicine composition comprises the following raw materials by weight: 3-6 parts of Polygonum multiflorum, 3-6 parts of Angelica sinensis, 2-5 parts of Oroxylum indicum, and 3-5 parts of Lycium barbarum. The composition has the effect of alleviating the growth environment of hair follicles and promoting the production of melanin by melanocytes. Furthermore, the composition utilizes nanotechnology to facilitate scalp absorption. The preparation method of the traditional Chinese medicine composition of the present invention is simple and convenient, and is conducive to large-scale production and application.

The invention belongs to the field of recombinant proteins. The invention provides recombinant human-derived XVII type collagen, a composition with the effects of growing hair, growing hair, preventing hair loss, fixing hair and turning white hair into black hair and application of the recombinant human-derived XVII type collagen, the amino acid sequence of the recombinant human-derived XVII type collagen is shown as SEG ID NO. 1, and the invention further provides the composition with the effects of growing hair, growing hair, preventing hair loss, fixing hair and turning white hair into black hair. The recombinant human XVII type collagen has remarkable effects of promoting melanogenesis and promoting hair growth, and tests show that the recombinant human XVII type collagen has a remarkable effect of promoting the activity of HaCat cells and HFDPC cells; the composition with the effects of growing hair, nourishing hair, preventing hair loss, fixing hair and turning white hair into black hair has the effect of strongly promoting melanocyte secretion, and the hair growing effect is obvious.

The invention relates to application of Ro5-4864 or pharmaceutically acceptable salt in preparation of a medicine for treating and / or preventing melanocyte degeneration diseases caused by inflammatory immune response induced by oxidative stress. The melanocyte degeneration diseases caused by inflammatory immune response induced by oxidative stress comprise vitiligo, leukoplakia and pityriasis albicans. The Ro5-4864 and the pharmaceutically acceptable salt of the Ro5-4864, disclosed by the invention, inhibit continuous loss of melanocytes from oxidative stress, are strong in targeting effect and relatively small in side effect, and have remarkable advantages and progress compared with existing medicines.

The invention discloses a composition for turning white hair into black hair and application of the composition. The composition for turning the white hair into the black hair is prepared from the following components in parts by mass: 1.5 to 5.0 parts of hydroxyethyl piperazine ethanesulfonic acid, 0.5 to 3.5 parts of arginine, 40.0 to 80.0 parts of glycoprotein, 24.0 to 40.0 parts of hydroxyferriheme and 0.1 to 0.6 part of vitamin C, the composition disclosed by the invention can penetrate into hair follicles to play a role, activate a plurality of MITF pathways in melanocytes in the hair follicles, induce melanin, an antioxidant and a stem cell protection factor generation receptor to react, effectively promote the function of the melanocytes, and effectively promote white hair to become black hair for a long time. Meanwhile, the composition for turning the white hair into the black hair can promote generation of fibroin of keratinocytes, scalp barrier repairing is achieved, and the combing performance of the hair and the brightness of the hair can be improved.

The peptides of the invention have a sequence with at least 85% identity with a sequence SEQ ID NO: 1 or 2.These peptides regulate melanocyte activity in terms of proliferation and production of melanin. Therefore, they are useful in the treatment of melanomas but also of cosmetic cutaneous hyper or hypopigmentation.

This invention belongs to the field of cosmetic technology, specifically relating to a composition with water-locking and brightening functions and its targeted microcarrier delivery system. The water-locking and brightening composition provided by this invention mainly consists of niacinamide, tranexamic acid, oat β-glucan, disodium EDTA, and sodium hyaluronate. This composition can achieve a synergistic effect of water locking and brightening, effectively improving dullness, dryness, and uneven skin tone. Simultaneously, this invention also provides a targeted microcarrier delivery system, which is prepared by encapsulating the above composition in an internal aqueous phase using liposome technology, and simultaneously covalently attaching a targeting ligand to the surface of the liposomes. The targeted microcarrier delivery system obtained by this invention can actively target melanocytes and keratinocytes, achieving precise delivery of brightening and water-locking ingredients, significantly improving skin tone and addressing the root causes of dullness and uneven skin tone.

The invention relates to a composition with skin whitening, brightening and soothing effects, a skin product and a preparation method of the skin product. The composition is prepared from hydrolyzed corn protein, bis-diethoxy diethylene glycol cyclohexane 1, 4-dicarboxylate, kappaphycus alvarezii extract and aminoethyl phosphinic acid according to the weight ratio of (0.1 to 5) to (0.5 to 2) to (0.3 to 1.5) to (0.5 to 1.5). Compared with the prior art, the composition has the advantages that through special selection of the functional composition, tyrosinase activity is inhibited, oxidation reaction in the melanogenesis process is inhibited, stimulation of ultraviolet rays to melanin cells is weakened, melanin generation is reduced, formation of melanin and color spots is inhibited through multi-aspect synergism, chromatosis is prevented, and the effect is remarkable. The skin whitening mask has the effects of whitening and brightening the skin color, and the keratinocyte morphology is better protected by increasing the formation of a keratinocyte envelope, so that the keratinocyte envelope is more full, more compact in arrangement and better in barrier function.

The invention provides a skin grafting device which comprises a skin taking structure and a driving part, the skin taking structure comprises a plurality of skin taking parts, each skin taking part comprises a tool bit and a first connecting part, the first connecting parts extend in the first direction, and the tool bits are connected to the ends, in the first direction, of the first connecting parts; the other end, in the first direction, of the first connecting part is rotatably connected to the driving part, the multiple skin taking parts are arranged at intervals in the direction perpendicular to the first direction, and the driving part is used for driving the skin taking parts to rotate around the axis parallel to the first direction. The multiple skin taking parts take skin at the same time, the skin taking efficiency is remarkably improved, in the rotation process of the skin taking parts, the tool bit extracts skin tissue, tiny autologous skin extracted by the skin taking parts carries hair follicles, sweat glands, melanocytes and the like, and the multiple skin taking parts carry the autologous skin to be implanted into allogeneic skin or artificial skin at the same time, so that a complete skin function reconstruction basis is provided, and the skin taking efficiency is improved. Therefore, the skin transplantation effect and the skin transplantation efficiency are improved, and meanwhile, a smaller wound is generated on a skin tissue donor region.