Regeneration material of dermis substitution for tissue engineering skin for loading rhGM-CSF and preparation method thereof

Abstract

The invention relates to a regeneration material of artificial dermis substitution and a preparation method thereof. In the regeneration material of a dermis substitution for tissue engineering skin for loading rhGM-CSF, the regeneration material is formed by a heparinizing collagen-chitosan bracket loading the rhGM-CSF solution. The invention further discloses a preparation method of the regeneration material. The invention provides an artificial dermis substitution with good performance for curing the whole skin coloboma of trauma, burning, chronic skin ulcer, thereby being capable of remarkably quickening the vascularization process of the regeneration material of artificial dermis substitution, decreasing the infection risk, promoting the healing of the surface of a wound and reducing the hyperplasia of scar, and thus easing the pain of patients. The invention can be widely applied for the aspects of trauma, burning, surgery reshaping, and the like. The preparing method is simple, the source of the material is wide, the production efficiency is high, and the invention is suitable for industrial production.

Description

technical field

[0001] The invention relates to a regenerative material for an artificial leather substitute and a preparation method thereof. Background technique

[0002] As the largest organ of the human body, skin is mainly composed of epidermis, dermis and subcutaneous tissue, and is also rich in blood vessels and nerves. Skin defects are easily caused in daily work and life due to various reasons such as trauma, burns, chronic diseases, surgery, etc. Although the skin tissue has a strong regenerative ability, in the case of a large area of ​​full-thickness skin defect, due to the loss of the dermis, the regenerative ability of the skin is significantly weakened, and a large number of scars or scar contractures are easily produced.

[0003] The key to treating full-thickness skin defects is to rebuild and regenerate dermal tissue, promote early wound closure, and avoid microbial invasion. At present, there are three main ways to deal with tissue defects, namely: autol...

Examples

Embodiment 2

[0036] Collagen and chitosan were made into 0.5% acetic acid solution with a mass fraction of 0.5%, and then the collagen solution and chitosan solution were mixed according to the volume ratio of 9:1, fully stirred evenly, and injected into a mold with a diameter of 2 cm to make it After the height reaches 1.5mm, place it at 4°C for 24 hours, freeze it at -20°C for 24 hours, and then place it in a lyophilizer for 16 hours; freeze-dried scaffolds are cross-linked under vacuum at 105°C for 24 hours; Heparin was added to 0.05M MES (pH5.4) of EDC (20mmmol) and N-hydroxysuccinimide (NHS) at a molar ratio of 2:1 based on a heparin-to-collagen mass ratio of 1:10 solution, and then place the lyophilized scaffold in the mixed solution for cross-linking for 24 hours, and then freeze-lyophilize again after repeated washing to obtain a cross-linked heparinized collagen-chitosan scaffold (for microstructure, see figure 1 ).

[0037] After the heparinized collagen-chitosan scaffold was st...

Embodiment 3

[0039] Collagen and chitosan were made into 0.5% acetic acid solution with a mass fraction of 0.5%, and then the collagen solution and chitosan solution were mixed according to the volume ratio of 9:1, fully stirred evenly, and injected into a mold with a diameter of 2 cm to make it When the height reaches 1.5mm, place it at 4°C for 24 hours, freeze it at -20°C for 24 hours, and then place it in a lyophilizer for 16 hours; freeze-dried scaffolds are cross-linked under vacuum at 105°C for 24 hours; Heparin was added to 0.05M MES (pH5.4) of EDC (20mmmol) and N-hydroxysuccinimide (NHS) at a molar ratio of 2:1 based on a heparin-to-collagen mass ratio of 1:10 solution, and then place the lyophilized scaffold in the mixed solution for cross-linking for 24 hours, and then freeze-lyophilize again after repeated washing to obtain a cross-linked heparinized collagen-chitosan scaffold (for microstructure, see figure 1 ).

[0040] After the heparinized collagen-chitosan scaffold was ste...

technical field

[0001] The invention relates to a regenerative material for an artificial leather substitute and a preparation method thereof. Background technique

[0002] As the largest organ of the human body, skin is mainly composed of epidermis, dermis and subcutaneous tissue, and is also rich in blood vessels and nerves. Skin defects are easily caused in daily work and life due to various reasons such as trauma, burns, chronic diseases, surgery, etc. Although the skin tissue has a strong regenerative ability, in the case of a large area of ​​full-thickness skin defect, due to the loss of the dermis, the regenerative ability of the skin is significantly weakened, and a large number of scars or scar contractures are easily produced.

[0003] The key to treating full-thickness skin defects is to rebuild and regenerate dermal tissue, promote early wound closure, and avoid microbial invasion. At present, there are three main ways to deal with tissue defects, namely: autol...

Examples

Embodiment 2

[0036] Collagen and chitosan were made into 0.5% acetic acid solution with a mass fraction of 0.5%, and then the collagen solution and chitosan solution were mixed according to the volume ratio of 9:1, fully stirred evenly, and injected into a mold with a diameter of 2 cm to make it After the height reaches 1.5mm, place it at 4°C for 24 hours, freeze it at -20°C for 24 hours, and then place it in a lyophilizer for 16 hours; freeze-dried scaffolds are cross-linked under vacuum at 105°C for 24 hours; Heparin was added to 0.05M MES (pH5.4) of EDC (20mmmol) and N-hydroxysuccinimide (NHS) at a molar ratio of 2:1 based on a heparin-to-collagen mass ratio of 1:10 solution, and then place the lyophilized scaffold in the mixed solution for cross-linking for 24 hours, and then freeze-lyophilize again after repeated washing to obtain a cross-linked heparinized collagen-chitosan scaffold (for microstructure, see figure 1 ).

[0037] After the heparinized collagen-chitosan scaffold was st...

Embodiment 3

[0039] Collagen and chitosan were made into 0.5% acetic acid solution with a mass fraction of 0.5%, and then the collagen solution and chitosan solution were mixed according to the volume ratio of 9:1, fully stirred evenly, and injected into a mold with a diameter of 2 cm to make it When the height reaches 1.5mm, place it at 4°C for 24 hours, freeze it at -20°C for 24 hours, and then place it in a lyophilizer for 16 hours; freeze-dried scaffolds are cross-linked under vacuum at 105°C for 24 hours; Heparin was added to 0.05M MES (pH5.4) of EDC (20mmmol) and N-hydroxysuccinimide (NHS) at a molar ratio of 2:1 based on a heparin-to-collagen mass ratio of 1:10 solution, and then place the lyophilized scaffold in the mixed solution for cross-linking for 24 hours, and then freeze-lyophilize again after repeated washing to obtain a cross-linked heparinized collagen-chitosan scaffold (for microstructure, see figure 1 ).

[0040] After the heparinized collagen-chitosan scaffold was ste...

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