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Tissue engineering skin containing muscle cell and preparation method thereof

A technology for tissue engineering skin and muscle cells, which is applied in the field of preparation of tissue engineering skin containing muscle cells, can solve the problem that there are no reports on the development and application of tissue engineering skin containing muscle cells, the skin does not have elasticity and mechanical strength, and the wound surface is affected Healing quality and speed and other issues to achieve the effect of improving healing quality, reducing scar hyperplasia and improving cosmetic effect

Inactive Publication Date: 2008-03-19
陕西艾尔肤组织工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on tissue-engineered skin only includes the epidermis and dermis, without muscle cells, and the constructed skin does not have ideal elasticity and mechanical strength, which affects the quality and speed of wound healing
[0004] At present, there are no reports on the development and application of tissue-engineered skin containing muscle cells at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1. Preparation of culture medium: DF12 commercial culture medium and fetal bovine serum were mixed as base solution according to the volume ratio of 9:1; then 2 ng of insulin, 10 μg of hydrocortisone, and 2 μg of epidermal growth factor were added according to the standard of 500 ml of base solution. Basic fibroblast growth factor 2ng, transforming growth factor β 10ng, adenine 12mg, transferrin 5mg, vitamin C 5mg, prepared as basal medium; then use the basal medium to prepare the following medium, and the concentration of the additives is the final concentration :

[0023] Medium a: basal medium + bovine pituitary extract 25μg / ml

[0024] Medium b: basal medium + calcium chloride 100μg / ml

[0025] Medium c: basal medium + calcium chloride 200μg / ml

[0026] Medium d: basal medium + calcium chloride 300μg / ml

[0027] Step 2. Preparation of bio-scaffold material: at 4°C, mix collagen 7: hyaluronic acid 2: chondroitin sulfate 1 by mass ratio, prepare it into a solu...

Embodiment 2

[0032] Step 1. Preparation of culture medium: DF12 commercial culture medium and fetal bovine serum were mixed as the base solution according to the volume ratio of 9:1; then 40 ng of insulin, 10 μg of hydrocortisone, and 8 μg of epidermal growth factor were added according to the standard of 500 ml of base solution. Basic fibroblast growth factor 8ng, transforming growth factor β 10ng, adenine 12mg, transferrin 5mg, vitamin C 20mg; prepared as basal medium. Then use the basal medium to prepare the following medium, and the concentration of the additives is the final concentration:

[0033] Medium a: basal medium + bovine pituitary extract 25μg / ml

[0034] Medium b: basal medium + calcium chloride 50μg / ml

[0035] Medium c: basal medium + calcium chloride 160μg / ml

[0036] Medium d: basal medium + calcium chloride 300μg / ml

[0037] Step 2. Preparation of bioscaffold material: at 4°C, mix collagen 10: hyaluronic acid 3: chondroitin sulfate 1 by mass ratio, prepare it into a ...

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Abstract

The invention provides a tissue engineering skin with myocyte and the preparation method of the tissue engineering skin. The invention has the characteristics that the prepared skin is made from the myocyte, skin fibroblast, skin epithelial cell and biomaterial scaffold; the invention is the tissue engineering skin with active myocyte, which is formed by compounding the myocyte with the skin fibroblast at the inside the biomaterial scaffold and planting the epithelial cell on the surface of the biomaterial scaffold. The prepared tissue engineering skin with myocyte is similar as the skin of human body and has the advantages of increasing the skin elasticity and mechanical performance, preventing body fluid flowing away, protecting the wound surface, promoting the wound surface healing and resisting the cicatrisation.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to the preparation of tissue engineering skin containing muscle cells. Background technique [0002] With the development of tissue engineering technology, tissue engineered skin has become a hot spot in the treatment of skin defects, but so far there is still no real ideal skin substitute to be applied. At present, in the fields of medical cosmetology, trauma, and burn surgery, with the requirements for functional recovery and aesthetic considerations, it is far from enough to achieve epithelialization of some deep skin defects, and people are still working hard to explore more. Reasonable method, so that the skin graft area can finally achieve the same or similar results as full-thickness skin grafting. The main problem currently existing is that the implanted artificial skin has a hard appearance and lacks elasticity for a long time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/38
Inventor 金岩张勇杰陆伟
Owner 陕西艾尔肤组织工程有限公司
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