36 results about "Biomaterial scaffold" patented technology

A layered manufacturing method of a three-dimensional microfluidic porous scaffold comprises the following steps: simulating the microstructure of a real organ, manufacturing a resin die with a microfluidic structure copying a blood vessel system, copying a negative mode of the microfluidic structure with silicon rubber, filling the negative mode with solution of biological materials, moving the silicon rubber die upwards to enable the solution of biological materials to be contacted with an ultra-low temperature flat plate vertically, moving the silicon rubber die downwards to realize demolding of the frozen structure of the biological materials after solidification of solution of the biological materials, realizing precision interlayer orientation as well as freezing, condensation and forming of the three-dimensional microfluidic porous scaffold by repeatedly performing filling of the solution of biological materials and moving up and down of the silicon rubber die, obtaining the three-dimensional frozen structure of the biological materials, and vacuum freeze drying the ultra-low temperature flat plate and the three-dimensional frozen structure of the biological materials, and finally obtaining the scaffold of the biological materials with a three-dimensional microfluidic system and an oriented porous structure. The layered manufacturing method has the advantage of forming a three-dimensional organ scaffold with a complex space microfluidic system and the oriented porous structure directly.

A preparation method for a tissue engineering transplant with a micro-nano topological geometry structure on the surface includes 1), preparing a silicone rubber seal with a micro-nano topological (three-dimensional) geometry structure pattern on the surface according to a micro molding method; 2), spraying a metal conducting nano-coating with the thickness of 50-100nm on the surface of the silicone rubber seal according to an ion sputtering method; 3), electrically spinning a natural or composite biological material solution onto the surface of the PDMS seal by an electrospinning technique toform a biological material support; 4), separating the spun biological material support from the PDMS seal to obtain the tissue engineering transplant with the micro-nano topological geometry structure on the surface. The preparation method has the advantages that the micro molding technology and the electrospinning technique are applied jointly, the spun biological material support is separatedfrom the PDMS seal easily, and the preparation method is simple and easy to operate and suitable for mass production.

The present invention relates to a process of making biodegradable and/or bioabsorbable biomaterials and keratin nonwoven fibrous articles by electrospinning fibers from a blend of biomaterials and keratin dissolved in organic solvents includes generating a high voltage electric field between oppositely charged biomaterials and keratin fluid in a syringe with a capillary tip and a metallic collection roller and causing a jet to flow to the roller as solvent evaporates and collecting fibrous membranes or scaffolds on the roller. Keratin increased the cell affinity of biomaterial scaffolds which have potential medical applications.

The invention belongs to the field of tissue engineering, and concretely discloses an application of adipose-derived stem cells in biological tissue repair. By using adipose-derived stem cells after induced differentiation at a three-dimensional biomaterial scaffold for proliferation, healing of injured skin can be accelerated, the healing time is shortened, a wound is flattened, skin wrinkle canbe reduced, a purpose of beautifying and healing the skin can be achieved, a better healing condition is provided for skin growth and recovery, and a reference is provided for stem cells used for clinical treatment of skin wound.

The invention relates to reinforce mechanical strength of biomaterial frame with surface finish method, which comprises: (1) dipping the frame into gelatine solution or chitose solution or their mixed solution; (2) taking out to dry in drying cabinet for 1-2h at 50~80Deg; (3) using EDC or glutaral solution for crosslinking reaction; (4) cleaning; (5) drying in baking oven at 40Deg to obtain the product. Compared with prior art, this invention increases the frame strength 6~12 times to 4-6MPa and fits to application in clinic and research.

The invention discloses a preparation method of a slow-release polypeptide growth factor biological material scaffold. The preparation method comprises the following steps: (1) uniformly mixing a biological material, a polypeptide growth factor and water for reaction to ensure that the biological material is swollen and the water exists in the biological material in three forms namely combined water, intermediate state water and free water, thereby obtaining a blending system; (2) performing freezing treatment on the blending system to obtain a frozen product; and (3) performing unfreezing treatment on the frozen product to obtain a biological material scaffold releasing the polypeptide growth factor. The slow-release polypeptide growth factor biological material scaffold prepared by the method disclosed by the invention can provide a supporting effect for the polypeptide growth factor to ensure that the polypeptide growth factor is released slowly in a long time, so that while the bioactivity of the polypeptide growth factor is ensured to the utmost extent, a long-time polypeptide growth factor slow release can be provided, thereby providing the polypeptide growth factor required by growth in a whole regeneration process for defective tissues.

The invention relates to a method for preparing a monoclonal antibody coating biomaterial scaffold. By adopting the method of electrostatic coating or anodic polarization, a bracket body is taken as an anode, an antibody ion with negative electricity is moved to the anode under the action of the electric field force, and is evenly coated on the surface of a biomaterial scaffold. In the method, extra drug elusion coating is not required to be prepared, no obvious interface exists, a layer of antibodies can be coated on the overall or partial surface of the bracket under the action of the static electricity, the stability of the coated antibody amount is good, the antibodies are not easy to be eluted, the usage is safe, the method is applicable to the industrial mass production, the operation is simple, and the cost is low.

The invention provides a recombinant adipose-derived stem cell over-expressing an Hpgds gene and a preparation method and application thereof, and belongs to the technical field of genetic engineeringmedicines. The recombinant adipose-derived stem cell is obtained by infecting an adipose-derived stem cell with a lentiviral vector expressing the Hpgds gene. The preparation method comprises the following steps of: constructing a lentiviral vector for expressing the Hpgds gene; extracting adipose-derived stem cells; and infecting the adipose-derived stem cells with the lentiviral vector expressing the Hpgds gene to obtain recombinant adipose-derived stem cells. The recombinant adipose-derived stem cells proliferate on a three-dimensional biomaterial scaffold, and can safely and durably express the immunomodulatory gene Hpgds so as to accelerate healing of diabetic damaged skin and shorten the healing time.

The invention discloses a three-dimensional cell assembly method based on a dielectrophoresis adsorption principle and belongs to the technical field of artificial manufacture of tissue and organs. According to the method, firstly, a conductive and degradable biological material is prepared to serve as a scaffold main body conducting layer for cell assembly, a non-conductive material with good biocompatibility is taken as a scaffold insulating layer, an artificial tissue organ layer information model with the material area calibrated is established with a computer, thus the information model is used for directly driving multi-sprayer rapid forming equipment to manufacture a conductive three-dimensional tissue engineering scaffold, the conductive three-dimensional tissue engineering scaffold is freeze-dried, electrode pairs are mounted on the sterilized three-dimensional scaffold, the three-dimensional scaffold is immersed into a selected cell suspension, alternating current with a certain amplitude value is conducted for a period of time, and three-dimensional cell assembly is realized. On the basis of the conductive degradable biological material scaffold and the dielectrophoresis adsorption principle, three-dimensional assembly of one or more kinds of cells can be realized.

The invention relates to a biodegradable nano yarn capable of inhibiting fibrosis, and preparation and application thereof. The biodegradable nano yarn is prepared from the following raw materials in parts by weight: 100 parts of I-type collagen/poly(lactic acid-caprolactone) composite solution and 100 parts of anti-fibrosis drug solution. The preparation method comprises the following steps: using the I-type collagen/poly(lactic acid-caprolactone) composite solution as a shell solution; and using the anti-fibrosis drug solution as a core solution, spinning, freezing over night, and carrying out freeze-drying. The nano yarn can be used as a biodegradable and absorbable biological material scaffold, and can be used for resisting fibrosis in the urethra tissue engineering restoration to prevent postoperative urethral restenosis and urethral submucoasl excessive fibrosis. The preparation method is simple and efficient, and is low in price. The material has the advantages of favorable mechanical properties, obvious anti-fibrosis effect, favorable biodegradability, favorable biocompatibility, and reasonable release rate and time.

This invention relates to biomaterials for directional tissue growth which comprise soluble fibres having a variable cross-sectional area which dissolve in a directional manner in situ, thereby controlling the direction and rate of formation of microchannels within the biomaterial and allowing vectored and timed channelling through the biomaterial. This may be useful, for example in directing the growth of blood vessels, nerves and other repair cells through the biomaterial in defined directions.

The present invention provides a multifunctional 2-D and 3-D matrix for propagation of stein cells, in particular, a cliitosatv-based biomaieria! scaffold is engineered to promote CNS regeneration from primitive neural precursors by stabilizing a recombinant protein, fibroblast growth factor to preserve the cardinal properties of stem cells. The matrix, is further modified by the addition of either the extracellular matrix protein fibronectiii or the small peptide RGD or IK.VAV. A method to manu&eture an injectable multifunctional microsphere scaffold is also disclosed that is suitable as a vehicle for cell transplantation to repair traumatic brain injuries.

A multi-chamber pellet die system to improve and increase analysis pellet manufacturing capabilities by allowing for multiple pellets or biomaterial scaffolds to be fabricated simultaneously.

The invention belongs to the technical field of biomedical materials, and particularly relates to a mechanical property enhanced acellular spinal cord biomaterial scaffold and a preparation method and application thereof. In order to overcome the problem that an existing acellular spinal cord scaffold is insufficient in mechanical property, a polylactic acid-glycolic acid copolymer (PLGA) shell is spun outside an acellular spinal cord (DSC) through an electrospinning technology, and the mechanical property enhanced acellular spinal cord biomaterial scaffold is constructed. Compared with a simple DSC scaffold, the biomaterial scaffold has the enhanced mechanical property and better anti-pressure ability, and collapse of transplanted materials and compression of the transplanted materials by surrounding tissue can be prevented; and research shows that when the biomaterial scaffold is transplanted to a rat total transection spinal cord injury area, spinal cord injury can be effectively repaired, the problem that the mechanical property of the acellular spinal cord is poor is solved, and in-situ repair of spinal cord injury is further promoted.

The present disclosure relates to compositions and methods for modulating wound healing and regeneration. More particularly, the present disclosure relates to immunomodulatory agents that promote wound healing and tissue regeneration, and that may be optionally used in combination with synthetic or biomaterial scaffolds.