A layered manufacturing method of a three-dimensional microfluidic
porous scaffold comprises the following steps: simulating the
microstructure of a real organ, manufacturing a resin die with a microfluidic structure
copying a
blood vessel system,
copying a
negative mode of the microfluidic structure with
silicon rubber, filling the
negative mode with solution of
biological materials, moving the
silicon rubber die upwards to enable the solution of
biological materials to be contacted with an ultra-low temperature flat plate vertically, moving the
silicon rubber die downwards to realize demolding of the frozen structure of the
biological materials after solidification of solution of the biological materials, realizing precision interlayer orientation as well as freezing, condensation and forming of the three-dimensional microfluidic
porous scaffold by repeatedly performing filling of the solution of biological materials and moving up and down of the
silicon rubber die, obtaining the three-dimensional frozen structure of the biological materials, and vacuum
freeze drying the ultra-low temperature flat plate and the three-dimensional frozen structure of the biological materials, and finally obtaining the
scaffold of the biological materials with a three-dimensional microfluidic
system and an oriented porous structure. The layered manufacturing method has the
advantage of forming a three-dimensional organ
scaffold with a complex space microfluidic
system and the oriented porous structure directly.