Preparation method for tissue engineering transplant with micro-nano topological geometry structure on surface

Abstract

A preparation method for a tissue engineering transplant with a micro-nano topological geometry structure on the surface includes 1), preparing a silicone rubber seal with a micro-nano topological (three-dimensional) geometry structure pattern on the surface according to a micro molding method; 2), spraying a metal conducting nano-coating with the thickness of 50-100nm on the surface of the silicone rubber seal according to an ion sputtering method; 3), electrically spinning a natural or composite biological material solution onto the surface of the PDMS seal by an electrospinning technique toform a biological material support; 4), separating the spun biological material support from the PDMS seal to obtain the tissue engineering transplant with the micro-nano topological geometry structure on the surface. The preparation method has the advantages that the micro molding technology and the electrospinning technique are applied jointly, the spun biological material support is separatedfrom the PDMS seal easily, and the preparation method is simple and easy to operate and suitable for mass production.

Description

technical field [0001] The invention belongs to the field of tissue engineering and regenerative medicine implantable medical biomaterials in human body, and in particular relates to a preparation method of tissue engineering grafts with micro-nano-nano topological geometric structures on the surface which can repair tissue damage. Background technique [0002] The micro-nano topology on the surface of tissue engineering grafts can better simulate the physical microenvironment of tissue or organ regeneration in space, and play an important role in promoting the repair of tissue or organ damage. At present, with the help of various physical and chemical processing methods, the processing of micro-nano topological structures on the surface of various inorganic metals or non-metallic biomaterials can be well realized, but there are still certain difficulties in the preparation of micro-nano patterns on the surface of natural or synthetic biomaterials. . The traditional micro-n...

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Problems solved by technology

However, this method has problems such as cumbersome preparation process, time-consuming and poor uniformity of nanofiber micropatterns, which greatly limits the application of this technology in large-scale preparation of nanofiber micropatterns.

In addition, the above method can only be used to prepare micro-nano micro-patterns in two dimensions, and cannot realize the preparation of three-dimensional micro-nano micro-patterns. However, many tissues in the human body, such as blood vessels, nerves, and tendons, are mostly three-dimensional structures. Micro-nano patterning technology

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