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Preparation method of monoclonal antibody coating biology stand

A monoclonal antibody and bio-stent technology, applied in liquid spraying equipment, stents, coatings, etc., can solve the problems of high restenosis rate, unsatisfactory long-term effect, strong thrombosis, etc., and achieve good stability and satisfy The effect of clinical needs and simple operation

Active Publication Date: 2009-01-07
LEPU MEDICAL TECH (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing drug-eluting stents use rapamycin or paclitaxel as the drug carried on the stent, but this kind of drug-eluting stent with a polymer coating still cannot fundamentally solve the problem of stents. The problem of internal thrombosis and restenosis has defects such as high restenosis rate after surgery and strong thrombosis, and the long-term effect is not ideal. Current research shows that this drug-eluting stent still has a restenosis rate of about 10%. In order to reduce Restenosis rate, the present researchers developed a monoclonal antibody bioscaffold

Method used

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  • Preparation method of monoclonal antibody coating biology stand
  • Preparation method of monoclonal antibody coating biology stand
  • Preparation method of monoclonal antibody coating biology stand

Examples

Experimental program
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Effect test

Embodiment 1

[0047] After polishing, 316 stainless steel alloy or cobalt complex alloy material was used to engrave a vascular stent with a laser, and the monoclonal antibody CD34-coated biological stent was prepared according to the following steps:

[0048] 1. Cleaning of the surface of the bracket body:

[0049] (1) Grinding the surface of the bracket body with an abrasive belt, mechanically removing oxides on the surface, and adjusting the surface roughness;

[0050] (2) Use ultrasonic waves to clean with 75% medical ethanol solution, 99.5% acetone solution, and deionized water in sequence. The ultrasonic frequency is 100KHz, and the cleaning time is 10 minutes;

[0051] (3) Soak the stent body in a 30g / L, 80°C sodium hydroxide solution for 10 minutes to remove grease and scale;

[0052] (4) Place the cleaned stent body in a vacuum dryer, set the temperature at 40° C., and take it out after drying for 45 minutes.

[0053] 2. Anti-corrosion treatment of the surface of the stent body: ...

Embodiment 2

[0060] After polishing, 316 stainless steel alloy or cobalt complex alloy material was used to engrave a vascular stent with a laser, and the monoclonal antibody CD133-coated biological stent was prepared according to the following steps:

[0061] 1. Cleaning of the surface of the bracket body:

[0062] (1) Grinding the surface of the bracket body with an abrasive belt, mechanically removing oxides on the surface, and adjusting the surface roughness;

[0063] (2) Use ultrasonic waves to clean with 75% medical ethanol solution, 99.5% acetone solution, and deionized water in sequence. The ultrasonic frequency is 28KHz, and the cleaning time is 15 minutes;

[0064] (3) Soak the stent body in a 60g / L, 70°C sodium hydroxide solution for 5 minutes to remove grease and scale;

[0065] (4) Place the cleaned stent body in a vacuum dryer, set the temperature at 30° C., and take it out after drying for 60 minutes.

[0066] 2. Anti-corrosion treatment on the surface of the bracket body:...

Embodiment 3

[0073] After polishing, 316 stainless steel alloy or cobalt complex alloy material is used to engrave a vascular stent with a laser; the surface of the stent is processed by chemical or physical methods, such as corrosion, anodic oxidation, micro-arc oxidation, micro-arc nitriding, etc. or a combination of these methods Holes are directly formed on the local surface of the raw material of the device body. The holes can be drug-loading grooves or pore structures, and the diameter of the holes is 100-800nm. Prepare the monoclonal antibody CD105-coated bioscaffold according to the following steps:

[0074] 1. Cleaning of the surface of the bracket body:

[0075] (1) Grinding the surface of the bracket body with an abrasive belt, mechanically removing oxides on the surface, and adjusting the surface roughness;

[0076] (2) Utilize ultrasonic waves to clean with 75% medical ethanol solution, 99.5% acetone solution, and deionized water successively, the ultrasonic frequency is 50KH...

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Abstract

The invention relates to a method for preparing a monoclonal antibody coating biomaterial scaffold. By adopting the method of electrostatic coating or anodic polarization, a bracket body is taken as an anode, an antibody ion with negative electricity is moved to the anode under the action of the electric field force, and is evenly coated on the surface of a biomaterial scaffold. In the method, extra drug elusion coating is not required to be prepared, no obvious interface exists, a layer of antibodies can be coated on the overall or partial surface of the bracket under the action of the static electricity, the stability of the coated antibody amount is good, the antibodies are not easy to be eluted, the usage is safe, the method is applicable to the industrial mass production, the operation is simple, and the cost is low.

Description

technical field [0001] The invention belongs to the field of biological scaffold preparation, and in particular relates to a preparation method of a monoclonal antibody-coated biological scaffold. Background technique [0002] In 1976, German scholar Andrea Gronzgo first proposed the idea of ​​installing a stent in the artery. By the 1990s, coronary artery stents had been widely used in clinical treatment. Vascular stents are the main means of interventional therapy for cardiovascular and peripheral vascular occlusive lesions. The cavity remains open. Vascular stents can be divided into bare stents, drug-eluting stents, polymer-coated stents, metal-coated stents, radioactive stents and artificial blood vessel-covered stents according to the surface state. The first stents used are basically bare stents. Since the stent is a heterogeneous substance relative to the blood vessel or other body pipelines, it stimulates the intima of the blood vessel to cause reactive hyperplasi...

Claims

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Application Information

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IPC IPC(8): A61L31/10B05B5/08A61F2/82
Inventor 陈永强余占江张正才蒲忠杰
Owner LEPU MEDICAL TECH (BEIJING) CO LTD
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