Layered manufacturing method of three-dimensional microfluidic porous scaffold

Abstract

A layered manufacturing method of a three-dimensional microfluidic porous scaffold comprises the following steps: simulating the microstructure of a real organ, manufacturing a resin die with a microfluidic structure copying a blood vessel system, copying a negative mode of the microfluidic structure with silicon rubber, filling the negative mode with solution of biological materials, moving the silicon rubber die upwards to enable the solution of biological materials to be contacted with an ultra-low temperature flat plate vertically, moving the silicon rubber die downwards to realize demolding of the frozen structure of the biological materials after solidification of solution of the biological materials, realizing precision interlayer orientation as well as freezing, condensation and forming of the three-dimensional microfluidic porous scaffold by repeatedly performing filling of the solution of biological materials and moving up and down of the silicon rubber die, obtaining the three-dimensional frozen structure of the biological materials, and vacuum freeze drying the ultra-low temperature flat plate and the three-dimensional frozen structure of the biological materials, and finally obtaining the scaffold of the biological materials with a three-dimensional microfluidic system and an oriented porous structure. The layered manufacturing method has the advantage of forming a three-dimensional organ scaffold with a complex space microfluidic system and the oriented porous structure directly.

Description

technical field [0001] The invention relates to the technical field of biomanufacturing of water-soluble biomaterial artificial organ scaffolds, in particular to a layered manufacturing method of porous scaffolds with three-dimensional microfluidic channels. Background technique [0002] By planting specific cells on biodegradable porous scaffolds, tissue engineering can construct biologically active and functional artificial organ substitutes in vitro or in vivo, which is expected to fundamentally solve the current contradiction between supply and demand in organ transplantation. At present, some breakthroughs have been made in the engineering manufacturing of artificial hearts, livers and lungs through decellularized organ scaffolds. The core is the use of natural extracellular matrix materials and the vascular network system of natural organs, which suggests that the traditional The material of the porous tissue engineering scaffold should be as close as possible to the e...

AI Technical Summary

Problems solved by technology

However, most of the biomaterials close to the extracellular matrix are water-soluble natural materials such as collagen, gelatin, chitosan, silk fibroin, etc., which have poor mechanical forming properties, and it is difficult to accurately manufacture a microfluidic system with spatial complexity. At present, most of the three-dimensional structures formed are porous scaffolds, planar microstructure scaffolds or three-dimensional scaffolds formed by manual stacking or wrapping