Full-implanted artificial corneal stroma and preparation method thereof

An artificial cornea, implantable technology, applied in the field of biomedical engineering, to achieve the effect of sufficient sources, wide distribution, avoiding toxic reactions and inducing rejection reactions

Inactive Publication Date: 2009-11-25
EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the use of adipose-derived stem cells as seed cells for the construction of artificial corneas at home and abroad

Method used

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  • Full-implanted artificial corneal stroma and preparation method thereof
  • Full-implanted artificial corneal stroma and preparation method thereof
  • Full-implanted artificial corneal stroma and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 In vitro preparation and detection of fully implantable artificial corneal stroma

[0051] 1. Experimental apparatus and reagents 6-month-old female New Zealand white rabbits, weighing 2-2.5 kg (Shanghai Animal Research Center of Chinese Academy of Sciences). DMEM (Dulbeco'smodified eagle'smedium) powder is the product of GIBCO Company in the United States; collagenase type I is the product of Worthington Company in the United States; fetal bovine serum (Fetal Bovine Serum, FBS) is the product of Hyclone Company in the United States; dexamethasone and transferrin are The product of Sigma Company in the United States; L-glutamine, ascorbic acid and trypsin are products of Shanghai Shisheng Biotechnology Co., Ltd.; penicillin and streptomycin are products of North China Pharmaceutical Co., Ltd.; NaHCO 3 It is a product of Shanghai Hongguang Chemical Factory. LSM-510 two-photon microscope was purchased from Zeiss, Germany. JSM-6701F scanning electron microscope...

Embodiment 2

[0061] The in vivo (animal) experiment of embodiment 2 fully implanted artificial corneal stroma

[0062] 1. Experimental apparatus 6-month-old female New Zealand white rabbits, weighing 2-2.5 kg (Shanghai Animal Research Center of Chinese Academy of Sciences). DMEM (Dulbeco's modified eagle's medium) powder is a product of GIBCO Company of the United States; collagenase type I is a product of Worthington Company of the United States; fetal bovine serum (Fetal Bovine Serum, FBS) is a product of Hyclone Company of the United States; dexamethasone and transferrin are products of Sigma Corporation of the United States. The company's products; L-glutamine, ascorbic acid and trypsin are products of Shanghai Shisheng Biotechnology Co., Ltd.; penicillin and streptomycin are products of North China Pharmaceutical Co., Ltd.; NaHCO 3 It is a product of Shanghai Hongguang Chemical Factory; vimentin monoclonal antibody was purchased from American Lab Vision; smooth muscle protein monoclon...

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Abstract

The invention belongs to the field of biomedical engineering, in particular to a novel full-implanted artificial corneal stroma and a preparation method thereof. The artificial corneal stroma uses free isolated adipose-derived stem cells as seed cells which are inoculated in polylactic acid polyglycolic acid copolymer scaffold materials and can absorb nourishment and excrete waste in a three-dimensional space formed by biomaterial scaffolds, and the suitable scaffold materials not only can maintain the positions and the forms of the seed cells, but also can participate the adjustment and the control of cell differentiation states. Proved by animal experiments, the corneal stroma has favorable biocompatibility, avoids the toxic reaction and the induced exclusive reaction of a traditional corneal stroma to a human body, and can be fully degraded over time after being implanted so that the cornea can recover the transparency, thereby satisfying the requirement of the human body to the vision.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and relates to a novel totally implantable artificial corneal stroma (totally implantable artificial corneal stroma) and a preparation method thereof. Background technique [0002] Blindness due to corneal burns, corneal ulcers, corneal leukoplakia and other corneal diseases is more common clinically. Allogeneic keratoplasty is the main and effective way to treat these patients with corneal blindness. At present, there are as many as 4 million corneal blind patients in my country. With the gradual development of laser surgery and the aging population, the source of allogeneic corneas is becoming increasingly scarce. Clinically, ophthalmologists have an increasingly urgent demand for donor corneas. Practice has shown that the long-term prognosis of high-risk keratoplasty using allogeneic cornea as a donor is poor. The destruction of the local immune microenvironment on the ocular surface is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/18A61F2/14
Inventor 徐建江洪佳旭
Owner EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV
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