Application of adipose-derived stem cells in biological tissue repair

A technology of adipose stem cells and biological tissues, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of limited donor skin for skin damage, unsatisfactory treatment effects, long skin healing time, etc., to shorten the healing time , less damage to the body, and easier to obtain materials

Inactive Publication Date: 2018-07-27
广州万智农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, skin damage is treated mainly through autologous skin grafts and allogeneic skin grafts. Clinically, autologous skin grafts are the main method of treatment. Limited and long skin healing time and scars after healing have caused a certain degree of impact on the lives of patients, resulting in unsatisfactory treatment effects

Method used

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  • Application of adipose-derived stem cells in biological tissue repair

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Embodiment 1

[0035] In this embodiment, the three-dimensional porous scaffold made of chitosan-polylactide is used as a bridge; it provides corresponding space and place for the value-added and metabolism of fat stem cells, and finally by checking the repair effect of damaged skin, By comparing with the fat stem cell group, the porous scaffold group, and the blank group (autologous repair), the function and effect of the fat stem cells on the repair of damaged skin tissue were analyzed and judged. details as follows:

[0036] 1. Isolation and culture of adipose-derived stem cells

[0037] 1.1 Isolation and culture of cells

[0038] (1) Isolation and culture of mouse adipose-derived stem cells

[0039] The adipose tissue in the subcutaneous groin of the dead mice was taken and washed with Hanks solution, and the adipose tissue was cut into pieces with scissors, then an appropriate amount of 0.16% type II collagenase was added and stirred, and digested at about 35°C for 35 minutes. Then t...

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Abstract

The invention belongs to the field of tissue engineering, and concretely discloses an application of adipose-derived stem cells in biological tissue repair. By using adipose-derived stem cells after induced differentiation at a three-dimensional biomaterial scaffold for proliferation, healing of injured skin can be accelerated, the healing time is shortened, a wound is flattened, skin wrinkle canbe reduced, a purpose of beautifying and healing the skin can be achieved, a better healing condition is provided for skin growth and recovery, and a reference is provided for stem cells used for clinical treatment of skin wound.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to the application of adipose stem cells in biological tissue repair. Background technique [0002] Cell culture technology refers to the growth of cells under in vitro conditions in which the cells no longer form tissues (animals). As a method of cultivating cells, cell culture technology has been deeply and widely used in the field of biology to observe the morphological structure and life activities of living cells and various disciplines such as cytology, genetics, immunology, experimental medicine and oncology. Research is of great significance to both clinical and basic research. [0003] Skin damage is one of the important causes of modern disease damage, many reasons can lead to skin damage, such as burns, scalds, external accidental trauma and skin diseases and other factors can lead to skin damage. Currently, skin damage is treated mainly through autologous skin grafts ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/38A61L27/60
CPCA61L27/26A61L27/3804A61L27/60A61L2430/34C08L5/08C08L67/04
Inventor 杨金源
Owner 广州万智农业科技有限公司
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