The invention discloses a standardized cultivation method of rhizoma bletillae, and belongs to the technical field of traditional Chinese medicinal material plantation. The standardized cultivation method of rhizoma bletillae comprises following steps: 1, seedling breeding; 2, field transplanting; 3, field cultivation growing period management; 4, harvesting and processing; and 5, packaging, storing, and transportation. Compared with the prior art, the standardized cultivation method possesses following advantages: 1, the standardized cultivation method is simple and practical, management is convenient, standardized technical specification is provided for steps from seedling breeding to packaging, storing, and transportation of rhizoma bletillae, and the standardized cultivation method is suitable for large-scale popularization and cultivation; 2, a set of rhizoma bletillae seedling breeding methods is provided, a defect of propagation by tuber that seed consumption amount is large is avoided, improvement of rhizoma bletillae seed germination induction, differentiation propagation, and rooting and strong seedling culture conditions is realized, rhizoma bletillae tissue culture plant survival rate is increased; 3, rhizoma bletillae seedling domestication cultivation measures are provided, and domesticated plant survival rate is increased; and 4, rhizoma bletillae culture medium formula ratio, bottle seedling, and domesticated plant seedling emergence standards are standardized, and lack of technology and standards in large-scale production is avoided.