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Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches

A technology of prothallium and bird's nest fern, which is applied in the field of agricultural biology, can solve the problems of irregular seedlings, high reproduction coefficient and low reproduction coefficient, and achieve the effects of consistent physiological age, simple process flow and tidy growth.

Active Publication Date: 2012-10-10
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

[0003] Under natural conditions, the propagation method of bird's nest fern is usually by branch propagation method, and spore propagation method can also be used, but the spore propagation requires high conditions, and it is difficult to differentiate and emerge
However, the use of tissue culture to propagate bird's nest fern can obtain large-scale neat bottle seedlings, and the reproduction coefficient is high. In recent years, the tissue culture of bird's nest fern has been multiplied by the method of differentiating seedlings from seedlings. The seedlings are not neat, and the seedlings that differentiate first are easy to age, which is not conducive to batch emergence

Method used

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  • Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches
  • Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches
  • Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches

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Embodiment 1

[0029] 1. Prothallium culture process

[0030] Take the bird's nest fern leaves with unspread spores, soak them in detergent solution for 10 minutes, rinse them for 10 minutes, and use 0.1% HgCl on a clean bench 2 Sterilize for 8 min, rinse with sterile water 4 times, absorb the water with sterile paper, scrape out the spores with a scalpel, and inoculate it into the spore germination medium (MS basic medium + BA0.5 mg / L + sucrose 30 g / L+carrageenan 6.5 g / L, pH 5.8), the spores germinate and grow into green protons after about 6-8 weeks of culture. Many small green spots can be seen on the surface of the medium; the spores are formed after germination Inoculated into the development medium (MS+6-BA 1.0 mg / L+NAA 0.02 mg / L+ sucrose 30 g / L+ carrageenan 6.5 g / L, pH 5.8). After 4 weeks, the profila developed into Prothallium.

[0031] 2. Proliferation and cultivation process

[0032] Cut the prothal body mass into 1 cm 3 Inoculate the small pieces of spores into the proliferation mediu...

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Abstract

The invention belongs to the field of agricultural biotechnology and relates to a method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches. The method comprises the steps of prothallium culture process, multiplication culture process, sporophyte induction process, rooting induction process, and transplant and manage process. The technological process is simple; by reproduction in the form of prothallium, a great amount of prothallium can be obtained; by the processes of multiplication, sporophyte induction, sporophyte rooting and transplant, a great number of seedlings capable of being used for production and culture can be obtained; the obtained seedlings are at the same physiological age and grow uniformly; and the method is suitable for industrial seedling raising and scale culture.

Description

Technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for rapidly breeding the seedlings of the bird's nest fern by using the prothallium multiplication approach. The method utilizes the prothallium induced by the spores of the fern fern, through the processes of proliferation, sporophyte induction, sporophyte rooting, and transplanting, to obtain a large number of seedlings that can be used for production and cultivation. Background technique [0002] Asplenium nidus (Asplenium nidus), also known as Shansu flower, Nestle fern, Nestle dentate, belongs to Asplenium nidus and is a perennial evergreen epiphytic fern. Produced in Asia, Africa and Australia. Bird's nest fern has the characteristics of evergreen, plump plant shape, elegant and beautiful leaf color and lush green color. It is a good foliage material for arranging halls, meeting places and making flower baskets. It uses a large amount of cutting le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 李志英徐立李腾敏
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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