Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches

A technology of prothallus and bird's nest fern, which is applied in the field of agricultural biology, can solve the problems of high reproduction coefficient, irregular seedlings, and low multiplication coefficient, and achieve the effect of simple process flow, consistent physiological age, and neat growth

Active Publication Date: 2013-10-23
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Under natural conditions, the propagation method of bird's nest fern is usually by branch propagation method, and spore propagation method can also be used, but the spore propagation requires high conditions, and it is difficult to differentiate and emerge
However, the use of tissue culture to propagate bird's nest fern can obtain large-scale neat bottle seedlings, and the reproduction coefficient is high. In recent years, the tissue culture of bird's nest fern has been multiplied by the method of differentiating seedlings from seedlings. The seedlings are not neat, and the seedlings that differentiate first are easy to age, which is not conducive to batch emergence

Method used

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  • Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches
  • Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches
  • Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches

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Embodiment 1

[0029] 1. Prothallus culture process

[0030] Take the bird's nest fern leaves with undissipated spores, soak them in detergent solution for 10 minutes, drain and rinse them for 10 minutes, and wash them with 0.1% HgCl on the ultra-clean workbench. 2 Sterilize for 8 minutes, rinse with sterile water for 4 times, blot dry with sterile paper, scrape out the spores with a scalpel, and inoculate them into the spore germination medium (MS basic medium + BA0.5 mg / L + sucrose 30 g / L+carrageenan 6.5 g / L, pH 5.8), the spores germinate and grow into green protonema after about 6 to 8 weeks of culture, and many green dots can be seen on the surface of the medium; the spores germinate and form The protonema were inoculated in the development medium (MS+6-BA 1.0 mg / L+NAA 0.02 mg / L+sucrose 30 g / L+carrageenan 6.5 g / L, pH 5.8), after 4 weeks, the protonema developed into prothallus.

[0031] 2. Proliferation culture process

[0032] Cut the prothallus mass into 1 cm 3 The small pieces of...

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Abstract

The invention belongs to the field of agricultural biotechnology and relates to a method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches. The method comprises the steps of prothallium culture process, multiplication culture process, sporophyte induction process, rooting induction process, and transplant and manage process. The technological process is simple; by reproduction in the form of prothallium, a great amount of prothallium can be obtained; by the processes of multiplication, sporophyte induction, sporophyte rooting and transplant, a great number of seedlings capable of being used for production and culture can be obtained; the obtained seedlings are at the same physiological age and grow uniformly; and the method is suitable for industrial seedling raising and scale culture.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a method for rapidly breeding bird's nest fern seedlings by using the prothallus multiplication pathway. The method utilizes the prothallus induced by the bird's nest fern spores to obtain a large number of seedlings that can be used for production and cultivation through processes such as multiplication, sporophyte induction, sporophyte rooting, and transplanting. Background technique [0002] Bird's Nest Fern (Asplenium nidus), also known as Shansu flower, Nestle fern, Nestle tooth, belongs to Asplenium nidus and is a perennial evergreen epiphytic fern. Native to Asia, Africa and Australia. Bird's nest fern has the characteristics of evergreen in four seasons, plump plant shape, elegant and beautiful, and green leaf color. It is a good foliage material for arranging halls, meeting places and making flower baskets. It consumes a lot of leaves and is als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 李志英徐立李腾敏
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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