A kind of tissue culture and breeding method of golden retriever spore spores

A technology of golden retriever ridge and ridge spore group, which is applied in the field of plant cultivation, can solve the problem that golden retriever dog ridge cannot be reproduced on a large scale, and achieve the effects of neat growth, robust plant growth, and less dosage

Active Publication Date: 2017-11-24
桂林亦元生现代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for tissue culture and breeding of golden dog ridge spores, which can be used for rapid breeding of golden dog ridge, and solves the problem that golden dog ridge cannot be propagated on a large scale

Method used

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  • A kind of tissue culture and breeding method of golden retriever spore spores
  • A kind of tissue culture and breeding method of golden retriever spore spores
  • A kind of tissue culture and breeding method of golden retriever spore spores

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Experimental program
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Effect test

Embodiment 1

[0065] The method for tissue culture and breeding of golden retriever chrysospores comprises the following steps:

[0066] 1), collect the mature sporangia on the sporophyll of golden retriever dog ridge and disinfect;

[0067] 2), sporangia are inoculated to N 6 Cultivate in culture medium until spores germinate; culture conditions are: light intensity 1000-1500lx, light time 7-9 hours / day, culture temperature 23-25°C, culture humidity 65-70%; culture conditions in the following steps are the same as this stage The culture conditions are the same;

[0068] 3), the germinated tissue is transferred to the Pragre medium and cultivated to induce the generation of prothallus;

[0069] 4), the prothallus is transferred to the new Pragre medium and cultivated, and induced to differentiate into young sporophytes;

[0070] 5), the young sporophytes are transferred to new Pragre medium and cultivated into sporophytes.

Embodiment 2

[0072] The method for tissue culture and breeding of golden retriever chrysospores comprises the following steps:

[0073] 1), collect the mature sporangia on the sporophyll of the golden retriever dog, and disinfect it with 0.1% mercury chloride solution on the ultra-clean workbench for 8-10 minutes;

[0074] 2) Connect the sterilized sporangia to the improved MS medium, crush the sporangia with clips, paste the spore powder on the improved MS medium and cultivate for 20-35 days until the spores germinate; the culture conditions are: light intensity 1000-1500lx, light time 7-9 hours / day, culture temperature 23-25°C, culture humidity 65-70%; the culture conditions in the following steps are the same as the culture conditions in this stage;

[0075] 3), transfer the tissue germinated from the sporangia to the improved 1 / 2MS medium and culture for 30-40 days, induce it to form a prothallus, and proliferate the prothallus at the same time, and the multiplication factor is 5-10 ti...

Embodiment 3

[0083] The method for tissue culture and breeding of golden retriever chrysospores comprises the following steps:

[0084] 1), collect the mature sporangia on the sporophyll of Golden Retriever, and disinfect with 5% sodium hypochlorite solution on the ultra-clean workbench for 8-10 minutes;

[0085] 2) Put the sterilized sporangia on the ultra-clean workbench, pad them with sterile filter paper, let the sporangia open, drop the spore powder onto the filter paper, shake off the spore powder on the filter paper and paste it on the improved MS+6-BA 0.2mg / L+NAA 0.1mg / L+agar 4.5g / L+sucrose 30g / L+activated carbon 0.2g / L culture medium for 20-35 days until spores germinate; culture conditions: light intensity 1000-1500lx , The light time is 7-9 hours / day, the culture temperature is 23-25°C, and the culture humidity is 65-70%; the culture conditions in the following steps are the same as the culture conditions in this stage;

[0086] 3), transfer the tissue germinated from the spora...

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Abstract

The present invention relates to the technical field of plant cultivation, in particular, relates to a method for tissue culture and breeding of the spores of the golden-haired dog's spore, comprising the following steps: 1), collecting mature sporangia on the leaves of the golden-haired dog's spore and disinfecting them; 2), dissolving the spores The sac was inoculated into the golden retriever ridge germination medium and cultivated until the spores germinated; 3), the germinated tissue was transferred to the golden retriever ridge proliferation medium and cultivated to induce the generation of clustered prothallus; 4), the prothallus Transfer to the new Golden Retriever Ridge differentiation medium for cultivation, and induce differentiation into young sporophytes; 5), transfer the young sporophytes to the golden retriever ridge rooting medium and cultivate them into sporophytes. The cultivation method can be used for rapid breeding of golden retriever ridges, large-scale neat seedlings can be obtained, and the reproduction coefficient is high, which solves the problem that golden retriever ridges cannot be propagated on a large scale.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for tissue culture and breeding of golden retriever spores. Background technique [0002] Golden dog ridge (Cibotium barometz (L.) J.Sm.) is a clamshell fern plant, mainly produced in Guangxi, and also distributed in Guangdong, Guizhou, Jiangxi, and Southeast Asia. It is a national II-level protected plant. [0003] Dog's spine is the dry rhizome of golden retriever dog's spine, which has the functions of dispelling rheumatism, nourishing the liver and kidney, strengthening the waist and knees, etc., and it has been recorded in the "Chinese Pharmacopoeia" in previous editions. Zhuang doctors and Yao doctors often use it to treat rheumatic arthralgia, soreness of the waist and knees, weakness of lower limbs and other diseases; Zhuang and Yao people often drink it with meat and bone foods, make tea with rhizomes, and stir-fry vegetables with young leaves Eating,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 苏钰琴黄云峰杨杰花莫燕兰蒋珍藕盘飞兰韦祥意罗文正秦丹欣张华英
Owner 桂林亦元生现代生物技术有限公司
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