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A method for rapidly propagating seedlings using four-leaf ginseng leaves

A four-leaf ginseng and leaf technology, which is applied in the field of plant tissue culture, can solve the problems of low differentiation rate, high mortality, and short growth, and achieve the effects of neat growth, simple process flow and consistent physiological age.

Active Publication Date: 2022-06-28
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Tiejun et al. used different explants such as bud-bearing shoot tips and stem segments of Taishan four-leaf ginseng to investigate the optimal tissue culture conditions of Taishan four-leaf ginseng, and compared the callus pathway with bud-bearing shoot tips and stem segments. The effect of clustering buds, the results of the study showed that the effect of induction of stem segments and shoot tips was not satisfactory
The differentiation rate induced by the stem segment with buds is extremely low, and the growth is slow. After the single seedling is subcultured after cutting, the growth is slow, or even not long, and the mortality rate is high; the spherical cauliflower-like callus is formed by the induction of the shoot tip, and the induced rate is only 20%

Method used

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  • A method for rapidly propagating seedlings using four-leaf ginseng leaves
  • A method for rapidly propagating seedlings using four-leaf ginseng leaves
  • A method for rapidly propagating seedlings using four-leaf ginseng leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Leaf-induced differentiation and adventitious bud process

[0032] Take the young four-leaf ginseng leaves, wash and sterilize them, dry the water with sterile paper, and inoculate the whole piece in the induction medium (MS+6-BA 0.8mg / L+NAA0.5mg / L+sucrose 30g / L+Kara Adventitious buds were induced to differentiate on the gelatin 6.5g / L, the pH of the medium was 5.8). Culture conditions: culture temperature 24~26℃, light culture, light time 10~12h / d, light intensity 20~30μmol m -2 ·s -1 . Culture for about 10 days, and start to differentiate adventitious buds at the incision of the leaf or on the surface of the leaf (see figure 1 ). After culturing for 20 days, they were cut into small pieces and inoculated on fresh induction medium for subculture.

[0033] 2. The cultivation process of strong seedlings

[0034] The adventitious buds after subculture proliferation were cut into small clusters (blocks) and transferred to strong seedling medium (MS+6-BA 0.5mg / L+NA...

Embodiment 2

[0044] 1. Leaf-induced differentiation and adventitious bud process

[0045]Take young four-leaf ginseng leaves, wash and sterilize them, dry the water with sterile paper, and cut them into small pieces of about 0.5cm × 1.0cm along the vertical direction of the leaf veins and inoculate them in the induction medium (MS+6-BA 2.5mg). / L+NAA0.1mg / L+sucrose 30g / L+carrageenan 6.5g / L, medium pH value is 5.8) to induce differentiated adventitious buds. Culture conditions: culture temperature 24~26℃, light culture, light time 10~12h / d, light intensity 20~30μmol m -2 ·s -1 . After about 10 days of culture, adventitious buds began to differentiate at the incision of the leaf or on the surface of the leaf. After culturing for 20 days, they were cut into small pieces and inoculated on fresh induction medium for subculture.

[0046] 2. The cultivation process of strong seedlings

[0047] Divide the adventitious buds after subculture into small clusters (blocks) and transfer to strong s...

Embodiment 3

[0057] 1. Leaf-induced differentiation and adventitious bud process

[0058] Take young four-leaf ginseng leaves, wash and sterilize them, and dry them with sterile paper, then cut them into small pieces of about 0.5cm × 1.0cm along the vertical direction of the veins and inoculate them in induction medium (MS+6-BA 2.0mg). / L+NAA0.3mg / L+sucrose 30g / L+carrageenan 6.5g / L, medium pH value is 5.8) to induce differentiated adventitious buds. Culture conditions: culture temperature 24~26℃, light culture, light time 10~12h / d, light intensity 20~30μmol m -2 ·s -1 . After about 10 days of culture, adventitious buds began to differentiate at the incision of the leaf or on the surface of the leaf. After culturing for 20 days, they were cut into small pieces and inoculated on fresh induction medium for subculture.

[0059] 2. The cultivation process of strong seedlings

[0060] Divide the adventitious buds after subculture into small clusters (blocks) and transfer to strong seedling ...

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Abstract

The invention belongs to the technical field of plant tissue culture, and relates to a method for rapidly propagating seedlings by using leaves of ginseng four leaves, including the processes of induction of leaves to differentiate adventitious buds, proliferation of adventitious buds, strong seedlings, induction of rooting, transplanting and management. The technological process of the present invention is simple. The leaves of Ginseng four-leaf ginseng are used as explants to directly induce the differentiation of adventitious buds, and the adventitious buds are subcultured to form cluster buds, strong seedlings, rooting of adventitious buds, transplanting and other processes, and a large number of usable buds can be obtained in a short time. It is suitable for the production of cultivated seedlings, and the obtained seedlings have the same physiological age and neat growth, which meets the market's demand for excellent four-leaf ginseng seedlings, is suitable for industrial seedling cultivation and large-scale cultivation, and lays the foundation for four-leaf ginseng breeding and industrial production. Base.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for breeding four-leaf ginseng seedlings, in particular to a method for rapidly propagating seedlings by using four-leaf ginseng leaves. The method utilizes four-leaf ginseng leaves to directly induce and differentiate adventitious buds, and obtains a large number of seedlings through the processes of adventitious bud proliferation, seedling growth, rooting induction, transplanting and the like. Background technique [0002] Four-leaf ginseng (Codonopsis lanceolata Benth.et Hook.F.), also known as Taishan ginseng, Radix Codonopsis, goat milk, milk ginseng, etc., is a plant of the Platycodonaceae Codonopsis genus. On hillside forest edges, sparse forest thickets and valleys. According to the records of "Taishan Medicine Records", four-leaf ginseng is listed as a "special product of ancient and modern", and it is one of the four famous medicines of Mount Tai. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/25A01G24/28A01G24/25A01G24/12
CPCA01H4/001A01H4/008A01G22/25A01G24/28A01G24/25A01G24/12
Inventor 符运柳李志英徐立王加宾孟春阳杨庆全朱振芬
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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