Method for rapidly propagating seedlings by using leaves of codonopsis lanceolata

A four-leaf ginseng and leaf technology, applied in the field of plant tissue culture, can solve the problems of slow growth, not long, unsatisfactory effect of stem segment and stem tip induction, etc.

Active Publication Date: 2021-07-06
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Tiejun et al. used different explants such as bud-bearing shoot tips and stem segments of Taishan four-leaf ginseng to investigate the optimal tissue culture conditions of Taishan four-leaf ginseng, and compared the callus pathway with bud-bearing shoot tips and stem segments. The effect of clustering buds, the results of the study showed that the effect of induction of stem segments and shoot tips was not satisfactory
The differentiation rate induced by the stem segment with buds is extremely low, and the growth is slow. After the single seedling is subcultured after cutting, the growth is slow, or even not long, and the mortality rate is high; the spherical cauliflower-like callus is formed by the induction of the shoot tip, and the induced rate is only 20%

Method used

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  • Method for rapidly propagating seedlings by using leaves of codonopsis lanceolata
  • Method for rapidly propagating seedlings by using leaves of codonopsis lanceolata
  • Method for rapidly propagating seedlings by using leaves of codonopsis lanceolata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Leaf induced differentiation of adventitious buds

[0032] Take young ginseng leaves, wash and disinfect them, blot dry with sterile paper, and inoculate the whole piece in induction medium (MS+6-BA 0.8mg / L+NAA0.5 mg / L+sucrose 30g / L+Kara Glue 6.5g / L, medium pH value is 5.8) to induce differentiation of adventitious buds. Culture conditions: culture temperature 24~26℃, light culture, light time 10~12h / d, light intensity 20~30μmol·m -2 ·s -1 . After culturing for about 10 days, adventitious buds began to differentiate at the leaf incision or on the surface of the leaf (see figure 1 ). After 20 days of culture, cut into small pieces and inoculate on fresh induction medium for subculture.

[0033] 2. Strong seedling cultivation process

[0034] Cut the adventitious buds after subculture into small clumps (blocks) and transfer them to strong seedling medium (MS+6-BA 0.5mg / L+NAA 0.1mg / L+sucrose 30g / L+carrageenan 6.5g / L+polypeptide 3ml / L) for strong seedling culture...

Embodiment 2

[0044] 1. Leaf induced differentiation of adventitious buds

[0045]Take young ginseng leaves, wash and sterilize, blot dry with sterile paper, then cut into small pieces about 0.5cm×1.0cm perpendicular to the veins and inoculate them in induction medium (MS+6-BA 2.5mg / L+NAA0.1mg / L+sucrose 30g / L+carrageenan 6.5g / L, medium pH value is 5.8) to induce differentiation of adventitious buds. Culture conditions: culture temperature 24~26℃, light culture, light time 10~12h / d, light intensity 20~30μmol·m -2 ·s -1 . After culturing for about 10 days, adventitious buds began to differentiate at the cuts of leaves or on the surface of leaves. After 20 days of culture, cut into small pieces and inoculate on fresh induction medium for subculture.

[0046] 2. Strong seedling cultivation process

[0047] Cut the adventitious buds after subculture into small clumps (blocks) and transfer them to strong seedling medium (MS+6-BA 0.3mg / L+NAA 0.5mg / L+sucrose 30g / L+carrageenan 6.5g / L+polypepti...

Embodiment 3

[0057] 1. Leaf induced differentiation of adventitious buds

[0058] Take young ginseng leaves, wash and sterilize, blot dry with sterile paper, then cut into small pieces of about 0.5cm×1.0cm perpendicular to the veins and inoculate them in induction medium (MS+6-BA 2.0mg / L+NAA0.3mg / L+sucrose 30g / L+carrageenan 6.5g / L, medium pH value is 5.8) to induce differentiation of adventitious buds. Culture conditions: culture temperature 24~26℃, light culture, light time 10~12h / d, light intensity 20~30μmol·m -2 ·s -1 . After culturing for about 10 days, adventitious buds began to differentiate at the cuts of leaves or on the surface of leaves. After 20 days of culture, cut into small pieces and inoculate on fresh induction medium for subculture.

[0059] 2. Strong seedling cultivation process

[0060] Cut the adventitious buds after subculture into small clumps (blocks) and transfer them to strong seedling medium (MS+6-BA 0.2mg / L+NAA 0.25mg / L+sucrose 30g / L+carrageenan 6.5g / L+poly...

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Abstract

The invention belongs to the technical field of plant tissue culture, and relates to a method for rapidly propagating seedlings by using leaves of codonopsis lanceolata. The method comprises the steps of induced differentiation of adventitious buds from the leaves, adventitious bud proliferation, seedling strengthening, rooting induction, transplanting and management. The technological process is simple, adventitious buds are directly induced and differentiated by taking the leaves of the codonopsis lanceolata as explants, the adventitious buds are subjected to subculture multiplication to form clumpy buds, seedlings are strengthened, the adventitious buds root, transplanting and the like, a large number of seedlings which can be used for production and cultivation can be obtained in a short time, and the obtained seedlings are consistent in physiological age, orderly in growth and high in yield. The requirements of the market for excellent seedlings of the codonopsis lanceolata are met, industrialized seedling culture and large-scale cultivation is suitable, and a foundation is laid for breeding and industrialized production of the codonopsis lanceolata.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a four-leaf ginseng seedling propagation method, in particular to a method for rapidly propagating four-leaf ginseng seedlings by utilizing four-leaf ginseng leaves. In the method, leaves of ginseng four-leaf ginseng are used to directly induce and differentiate adventitious buds, and a large number of seedlings are obtained through processes such as subculture and multiplication of adventitious buds, seedling strengthening, rooting induction, and transplanting. Background technique [0002] Four-leaf ginseng (Codonopsis lanceolata Benth.et Hook.F.), also known as Taishan ginseng, Codonopsis lanceolata, goat milk, milk ginseng, etc., is a plant of the genus Codonopsis in the Campanulaceae family. It is a herbaceous winding vine. The whole plant has white juice. On hillside forest margins, sparse forests, shrubs and valleys. According to the records of "Taishan Medicin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G22/25A01G24/28A01G24/25A01G24/12
CPCA01H4/001A01H4/008A01G22/25A01G24/28A01G24/25A01G24/12
Inventor 符运柳李志英徐立王加宾孟春阳杨庆全朱振芬
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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