A kind of identification method of potato scab resistance

Abstract

The invention belongs to the technical field of plant diseases, in particular to a method for identifying potato scab resistance. The invention provides a method for identifying potato scab disease resistance, which comprises the following steps: performing rooting culture and inducing culture of mini-potatoes on potato virus-free seedlings, inoculating fermentation broth of scab pathogenic bacteria during the induction culture, and continuing the induction culture The production of mini-potatoes, the incidence of plants and mini-potatoes were observed during the process, and the resistance to potato scab was judged according to the area of ​​micro-potatoes and the necrosis grade of the plants. According to the mechanism that scab pathogenic bacteria use pathogenic toxins to infect potato tubers to cause disease, the invention combines the techniques of tissue culture of potato virus-free seedlings and liquid-induced mini-potatoes to identify the resistance of potato scab disease. The identification period of the identification method provided by the invention is controlled at about 40 days, which greatly shortens the identification period.

Description

technical field [0001] The invention belongs to the technical field of plant diseases, in particular to a method for identifying potato scab resistance. Background technique [0002] Potato scab (Potato scab) is an important disease caused by soil Streptomyces. Among them, Streptomyces.Scabies, Streptomyces.acidiscabies, which cause acid scab, and Streptomyces.turgidiscabies, which cause convex scab lesions, are the three most common potato scab species, and the research is relatively concentrated. The epidermis of potato tuber infected with scab will produce brown spots, which will gradually expand, the tissue around the infection point will gradually necrosis, the surface of the tuber will become rough, and the tissue will become cork, which will seriously affect the quality of potatoes. According to statistics, the occurrence of potato scab is more serious in areas with alkaline soil, continuous cropping for many years, and vermiculite-based greenhouse mini-potato produc...

AI Technical Summary

Problems solved by technology

[0003] Since scab is a soil-borne disease that harms potato tubers, the disease occurrence process cannot be directly observed. It is necessary for potatoes to complete the whole growth period, and the identification results can be observed after the tubers are mature and harvested. It is impossible to know the infection of pathogenic bacteria on the host during the period when the tubers are not harvested. Identification methods are less controllable

Commonly used scab resistance identification methods are field planting potato inoculation pathogen identification method and pot plant inoculation pathogen spore suspension identification method. Field inoculation identification is close to natural occurrence, but the identification cycle is the whole growth period of potatoes. According to the different maturity time of potato varieties, identification The period is as long as 90-120 days. At the same time, the identification process is complicated to operate, requires a large amount of labor, needs to establish a disease nursery, and has the disadvantages of causing pathogenic bacteria contamination in the field.

The inoculation identification of greenhouse potted plants is closer to natural occurrence, and the scope of pathogenic bacteria contamination is controllable, but it also needs to be identified for the whole growth period of potatoes, which has disadvantages such as long cycle, high cost, and complicated field management.

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