A kind of identification method of potato scab resistance

A potato scab and identification method technology, applied to instruments, test plants/trees, measuring devices, etc., can solve the problems of low controllability of identification methods, contamination of field pathogens, and inability to directly observe, etc., to ensure consistency and reliability Repeatability, standardized detection operation process, and high identification accuracy

Active Publication Date: 2022-07-08
宁夏农林科学院农业生物技术研究中心
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Problems solved by technology

[0003] Since scab is a soil-borne disease that harms potato tubers, the disease occurrence process cannot be directly observed. It is necessary for potatoes to complete the whole growth period, and the identification results can be observed after the tubers are mature and harvested. It is impossible to know the infection of pathogenic bacteria on the host during the period when the tubers are not harvested. Identification methods are less controllable
Commonly used scab resistance identification methods are field planting potato inoculation pathogen identification method and pot plant inoculation pathogen spore suspension identification method. Field inoculation identification is close to natural occurrence, but the identification cycle is the whole growth period of potatoes. According to the different maturity time of potato varieties, identification The period is as long as 90-120 days. At the same time, the identification process is complicated to operate, requires a large amount of labor, needs to establish a disease nursery, and has the disadvantages of causing pathogenic bacteria contamination in the field.
The inoculation identification of greenhouse potted plants is closer to natural occurrence, and the scope of pathogenic bacteria contamination is controllable, but it also needs to be identified for the whole growth period of potatoes, which has disadvantages such as long cycle, high cost, and complicated field management.

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  • A kind of identification method of potato scab resistance
  • A kind of identification method of potato scab resistance
  • A kind of identification method of potato scab resistance

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preparation example Construction

[0031] In the present invention, the preparation method of the fermentation broth of the scab pathogenic bacteria preferably comprises: taking a concentration of 10 6 100 μl of the bacterial suspension of Streptomyces. Scabies, a strong pathogenic strain of potato scab with cfu / mL, was cultured in 100 ml of tryptic soy liquid medium, and cultured with shaking at 26-30°C and 180-250rpm for 5-7 days , after filtering the mycelium, the fermentation broth of scab pathogenic bacteria was obtained. The fermented liquid preparation method used in the invention can improve the efficiency of potato infection with scab disease after inoculation with the fermented liquid of scab pathogenic bacteria.

[0032] In the present invention, the potato virus-free seedlings are preferably stem-tip virus-free tissue culture seedlings from the same potato. The plants taken in the invention are virus-free seedlings with the same physiological age, and with the standardized operation process, the in...

Embodiment 1

[0053] The S. scabies strain was cultured in Gao's No. 1 medium for 7 days, and the spores were washed with sterile water to prepare a concentration of 10 6 cfu / mL bacterial suspension, take 100 μl of bacterial suspension and add 100 ml of sterilized Tryptic SoyBroth (TSB) culture medium (TSB finished product 30 g, distilled water 1000 mL), 28 ° C, 180 rpm , Shake culture for 7d, after the fermentation filtrate was filtered mycelium, the obtained scab bacteria fermentation filtrate was placed in a 4°C refrigerator for preservation.

[0054] Take potato virus-free seedlings (Japanese anti-scab, Aquila, 672-50, 16-8, 897003, Z292, bl-1c and EarlyOhio original seeds, provided by the National Potato Improvement Center, Keshan Branch, Heilongjiang Academy of Agricultural Sciences, Ningshu 15 No. (provided by Guyuan Branch of Ningxia Academy of Agriculture and Forestry Sciences) cut the normal and tidy stem segments, inoculated into the rooting culture of sucrose 30g / L, MS 4.42g / L, ...

Embodiment 2

[0057] The same test as in Example 1 was carried out, except that the fermentation filtrate of scab bacteria was inoculated with 600ul per test tube, and 600ul of TSB sterile culture medium was used as the control (CK).

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Abstract

The invention belongs to the technical field of plant diseases, in particular to a method for identifying potato scab resistance. The invention provides a method for identifying potato scab disease resistance, which comprises the following steps: performing rooting culture and inducing culture of mini-potatoes on potato virus-free seedlings, inoculating fermentation broth of scab pathogenic bacteria during the induction culture, and continuing the induction culture The production of mini-potatoes, the incidence of plants and mini-potatoes were observed during the process, and the resistance to potato scab was judged according to the area of ​​micro-potatoes and the necrosis grade of the plants. According to the mechanism that scab pathogenic bacteria use pathogenic toxins to infect potato tubers to cause disease, the invention combines the techniques of tissue culture of potato virus-free seedlings and liquid-induced mini-potatoes to identify the resistance of potato scab disease. The identification period of the identification method provided by the invention is controlled at about 40 days, which greatly shortens the identification period.

Description

technical field [0001] The invention belongs to the technical field of plant diseases, in particular to a method for identifying potato scab resistance. Background technique [0002] Potato scab (Potato scab) is an important disease caused by soil Streptomyces. Among them, Streptomyces.Scabies, Streptomyces.acidiscabies, which cause acid scab, and Streptomyces.turgidiscabies, which cause convex scab lesions, are the three most common potato scab species, and the research is relatively concentrated. The epidermis of potato tuber infected with scab will produce brown spots, which will gradually expand, the tissue around the infection point will gradually necrosis, the surface of the tuber will become rough, and the tissue will become cork, which will seriously affect the quality of potatoes. According to statistics, the occurrence of potato scab is more serious in areas with alkaline soil, continuous cropping for many years, and vermiculite-based greenhouse mini-potato produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
CPCG01N33/0098Y02A40/10
Inventor 聂峰杰巩檑甘晓燕张丽徐利敏刘璇任怡莲杨文静陈虞超宋玉霞黄新玲
Owner 宁夏农林科学院农业生物技术研究中心
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