A kind of tissue culture rapid propagation method of bird's nest fern

A technology of bird's nest fern and tissue culture rapid propagation, applied in the field of agricultural biology, to achieve the effects of consistent physiological age, neat growth, and simple technological process

Active Publication Date: 2018-01-16
海南大信园林股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no method suitable for tissue culture and rapid propagation of Bird's Nest fern, directly from leaf tissue culture

Method used

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  • A kind of tissue culture rapid propagation method of bird's nest fern
  • A kind of tissue culture rapid propagation method of bird's nest fern
  • A kind of tissue culture rapid propagation method of bird's nest fern

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Callus induction culture: use the unexpanded tender leaves of Bird’s nest fern as explant material, wash with an appropriate amount of detergent, rinse in running water for 0.5h, and sterilize the surface in 0.1% mercuric chloride solution. Bacteria for 7 minutes, washed 3 times in sterile water, cut into 1×1cm size and inoculated on the initial induction medium. The composition of the induction medium is: 1 / 2MS modified medium + 2.0mg / L6-BA + 1.0mg / L NAA , treatment inoculated 30 explants and repeated 3 times. Callus was induced for 45 days, and the callus induction rate was counted.

[0022] Callus induction rate=the number of explants that induced callus / the number of uncontaminated explants×100%.

[0023] 2. Proliferation culture: the callus is inoculated on the proliferation medium, and the composition is: MS basic medium + 1.0 mg / L 6-BA + 0.10 mg / L NAA. Treat and inoculate 50 explants, repeat 3 times, observe after 30 days, and count the proliferation rate. ...

Embodiment 2

[0031] 1. Callus induction culture: use the unexpanded tender leaves of Bird’s nest fern as explant material, wash with an appropriate amount of detergent, rinse in running water for 0.5h, and sterilize the surface in 0.1% mercuric chloride solution. Bacteria for 10 minutes, washed 3-5 times in sterile water, cut into 1×1cm size and inoculated on the initial induction medium. The composition of the induction medium is: 1 / 2MS modified medium + 1.0mg / L 6-BA + 0.5mg / L NAA, treatment inoculated 50 explants, and repeated 3 times. Callus was induced for 45 days, and the callus induction rate was counted. Callus induction rate=the number of explants that induced callus / the number of uncontaminated explants×100%.

[0032] 2. Proliferation culture: the callus is inoculated on the proliferation medium, and the composition is: MS basic medium + 1.0 mg / L 6-BA + 0.1 mg / L NAA. Treat and inoculate 50 explants, repeat 3 times, observe after 30 days, and count the proliferation rate. The e...

Embodiment 3

[0040] The difference between Example 3 and Example 1 is: the induction medium in the callus induction culture step is 1 / 3 MS improved medium; the rooting medium in the rooting culture step is also 1 / 3 improved medium.

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Abstract

The invention discloses a tissue culture fast propagation method of neottopteris nidus avis. The method comprises the following steps of: callus tissue induction: after explant material cleaning, surface sterilization is performed in a mercuric chloride solution for 7-10min; water is used for flushing; explant materials are cut into blocks and are inoculated into an induction culture medium; after 45 days, callus tissues are generated through induction; multiplication culture: the callus tissues are inoculated into a multiplication culture medium for multiplication; sporophore induction: the callus tissues subjected to multiplication culture are inoculated into a differentiation culture medium, and after the treatment is performed for 30 days, germinant sporophores are obtained; rooting culture: after leaves of the sporophores grow to 2 to 3cm, the sporophores are inoculated in a rooting culture medium for seedling culture; after 30 days, brown roots grow from the base parts of seedlings; acclimatization and transplanting: after 2 to 3 roots grow from each seedling, and the height is 4cm or more, the acclimatization and transplanting are performed. Through culture medium formula improvement, the induction rate of the callus tissues is 65.29 percent; the multiplication rate is 8.71 percent; the differentiation rate reaches 88.35 percent; the rooting rate reaches 91.21 percent; the survival rate reaches 95.3 percent or more.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Bird's nest fern. Background technique [0002] Bird's nest fern is a medium-sized epiphytic fern with the Latin name Neottopteris nidus cv. "Avis". It is a round-leaved potted species domesticated from Asplenium australasicum. The rhizome is short and erect, the stalk is thick and densely grown, and large groups of spongy fibrous roots can absorb a lot of water. The leaves are clustered, arranged radially on the top of the rhizome, hollow like a nest structure, and can collect fallen leaves and bird droppings; the leathery leaves are broad-lanceolate, smooth on both sides, slightly raised on both sides of the veins, and the sporangia are long strips. [0003] Bird's nest fern has the characteristics of evergreen in four seasons, plump plant shape, elegant and beautiful, and light green leaves. It is mainly used as a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 武华周冷青云胡悦祥寸德志
Owner 海南大信园林股份有限公司
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