The invention discloses a special biological bacterial fertilizer for strawberries as well as a preparation method and application thereof. The preparation method comprises the steps of with 8 parts of a bacillus cereus bacterial agent, 5 parts of a bacillus subtilis bacterial agent, 5 parts of a trichoderma bacterial agent, 30 parts of an organic fertilizer, 12 parts of a nitrogen fertilizer, 12parts of a nitrogen fertilizer, 12 parts of a phosphate fertilizer, 12 parts of a potassium fertilizer, 5 parts of a microelement fertilizer, 2 parts of plant growth promoting substances, 4 parts of humic acid and 5 parts of bentonite in parts by weight as the raw materials, stirring, mixing, sieving, drying, and carrying out packaging, so as to obtain the special biological bacterial fertilizer for the strawberries. The special biological bacterial fertilizer is applied to the aspects of increasing the yield of the strawberries, improving the quality of the strawberries, improving the storageproperty of the strawberries, inducing and improving the disease resistance of the strawberries, promoting the growth of low-age and forested strawberry plants, increasing the number of root microorganisms of the strawberries and improving the soil enzyme activity. The special biological bacterial fertilizer has the advantages of remarkable effect, environmental friendliness, no pollution and high utilization rate of fertilizer, the soil is improved, the diseases are prevented and controlled, the yield is increased, the quality is improved, the cost is low, and the popularization is easy.