Method for improving oil yield of microalgae

Abstract

The invention discloses a method for improving oil yield of microalgae. The method comprises the following steps: firstly, performing photoautotrophic cultivation on the microalgae in an improved culture medium containing a plant hormone, and at the stable phase, performing light stress and nitrogen stress cultivation on the microalgae obtained by centrifuging as seeds in a stress culture medium. The method for improving the oil yield of the microalgae has the advantages that the demand on nutrient substances in the cultivation process is reduced so that the cultivation cost can be reduced, and the biomass and the oil yield of chlorella can be improved simultaneously; the cultivation method is simple and easy to operate, and cannot be polluted easily; besides, the method has few influence factors and is suitable for large-scale cultivation.

Description

technical field [0001] The invention relates to a method for increasing the oil yield of microalgae, which belongs to the field of bioengineering. Background technique [0002] Microalgae is a type of small single-celled algae, which has the characteristics of high oil content, fast growth, high photosynthetic efficiency, strong environmental adaptability, and does not occupy arable land. The oil in the microalgae can generate fatty acids through transesterification Methyl ester, that is, biodiesel, is considered to be the most potential raw material for the development of biodiesel. Although microalgae has so many advantages, the cost of using microalgae to produce biodiesel is higher than that of petroleum-based fossil fuels. Among them, obtaining microalgae germplasm resources with high oil yield is one of the key steps in the development of biodiesel. one. [0003] Microalgae with high oil yield need the ability of microalgae to accumulate high biomass and oil content....

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Problems solved by technology

[0004] Chen Bilian et al. (CN 101016514A) can increase the biomass of microalgae by 10% to 60% by microwave treatment of microalgae, but this method requires a lot of equipment investment, high energy consumption and high production cost; Zhang Lin et al. Add hydroxyl-containing three-carbon organic matter in the photoautotrophic culture microalgae, so that the biomass of Chlorella can be increased to more than 500mg / L; Li Yuanguang et al. (CN 102154110A) through the first heterotrophic and then autotrophic culture steps, make ordinary small balls The algae biomass reaches more than 2g / L, and the oil yield reaches more than 150mg / L·d; Wang Yan et al. (CN 103045478A) made the biomass of oleaginous Chlorella up to 18g / L by alternately adding carbon source or nitrogen source, and the oil yield The content is as high as 48%; the above-mentioned methods can significantly increase the biomass of microalgae through heterotrophic or multitrophic methods, but it is easy to infect bacteria in the culture, the required equipment is relatively high, and the energy consumption is relatively high, and this method is not suitable Outdoor large-scale cultivation of microalgae

Compared with heterotrophic and concurrent cultures, photoautotrophic microalgae has a simpler cultivation process, lower requirements for equipment and nutrition, and less cost input, but the oil yield of microalgae is not as high as the above two methods. It is particularly important to rapidly increase the oil yield of photoautotrophic microalgae

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