Method for improving oil yield of microalgae

A microalgae oil and oil production rate technology, applied in the field of bioengineering, can solve the problems of easy bacterial contamination, high production cost, and high equipment requirements, and achieve the effects of simultaneously increasing biomass and oil content, low cost, and simple operation

Inactive Publication Date: 2014-12-10
SHIHEZI UNIVERSITY
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Problems solved by technology

[0004] Chen Bilian et al. (CN 101016514A) can increase the biomass of microalgae by 10% to 60% by microwave treatment of microalgae, but this method requires a lot of equipment investment, high energy consumption and high production cost; Zhang Lin et al. Add hydroxyl-containing three-carbon organic matter in the photoautotrophic culture microalgae, so that the biomass of Chlorella can be increased to more than 500mg/L; Li Yuanguang et al. (CN 102154110A) through the first heterotrophic and then autotrophic culture steps, make ordinary small balls The algae biomass reaches more than 2g/L, and the oil yield reaches more than 150mg/L·d; Wang Yan et al. (CN 103045478A) made the biomass of oleaginous Chlorella up to 18g/L by alternately adding carbon source o

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  • Method for improving oil yield of microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take an appropriate amount of C.vulgaris XJB algae liquid and inoculate it into a 1L Erlenmeyer flask containing 600mL of medium. The inoculum amount of the algae liquid is 10%, and the medium is: NaNO 3 0.83g / L,K 2 HPO4·3H 2 O 0.09g / L, MgSO 4 ·7H 2 O 0.12g / L, CaCl 2 27.2mg / L, Citric-acid 6.6mg / L, Fe(NH 4 ) 3 (C 6 h 5 o 7 ) 2 6.0mg / L, Na 2 EDTA·2H 2 O 1.1mg / L, NaCO 3 20mg / L, A5 trace element mixture 0.2mL / L, NaHCO 3 2.34g / L. Add 0.08mg / L triindoleacetic acid IAA to the basal medium, culture in photoautotroph at temperature 24±2°C, light intensity 6000±200lux, photoperiod 24:0 (L:D), and shake the flask three times a day.

[0023] According to the growth curve drawn by the number of cells regularly detected every day, the algae liquid was centrifuged at 4000r / min for 5 minutes in the stationary phase to obtain concentrated algae, and washed 5 times with distilled water, and all suspended in NaNO after washing away inorganic salt ions 3 Concentration i...

Embodiment 2

[0028] The photoautotrophic culture process of Chlorella and IAA used in this embodiment is the same as that in Embodiment 1.

[0029] Among them, the algae sludge obtained after IAA treatment is suspended under stress conditions, that is, NaNO 3 Concentration is 0.20g / L, other nutrient components are in the stress medium of the culture medium described in Example 1, under the conditions of 24 ± 2 ℃ under the light intensity is 8000lux, photoperiod is 24:0 (L:D) , stress culture for 2 days.

[0030] After stress cultivation to the stable stage, the detection and calculation of biomass, oil content, and oil yield are the same as in Example 1. After the above-mentioned IAA photoautotrophy---light and nitrogen stress culture, the oil yield of C.vulgaris XJB can reach 57.9 mg / L·d, which was increased by 2.8 times compared with the condition of only IAA culture, and increased by 6.5 times compared with the condition of no IAA or stress culture.

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Abstract

The invention discloses a method for improving oil yield of microalgae. The method comprises the following steps: firstly, performing photoautotrophic cultivation on the microalgae in an improved culture medium containing a plant hormone, and at the stable phase, performing light stress and nitrogen stress cultivation on the microalgae obtained by centrifuging as seeds in a stress culture medium. The method for improving the oil yield of the microalgae has the advantages that the demand on nutrient substances in the cultivation process is reduced so that the cultivation cost can be reduced, and the biomass and the oil yield of chlorella can be improved simultaneously; the cultivation method is simple and easy to operate, and cannot be polluted easily; besides, the method has few influence factors and is suitable for large-scale cultivation.

Description

technical field [0001] The invention relates to a method for increasing the oil yield of microalgae, which belongs to the field of bioengineering. Background technique [0002] Microalgae is a type of small single-celled algae, which has the characteristics of high oil content, fast growth, high photosynthetic efficiency, strong environmental adaptability, and does not occupy arable land. The oil in the microalgae can generate fatty acids through transesterification Methyl ester, that is, biodiesel, is considered to be the most potential raw material for the development of biodiesel. Although microalgae has so many advantages, the cost of using microalgae to produce biodiesel is higher than that of petroleum-based fossil fuels. Among them, obtaining microalgae germplasm resources with high oil yield is one of the key steps in the development of biodiesel. one. [0003] Microalgae with high oil yield need the ability of microalgae to accumulate high biomass and oil content....

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
Inventor 徐小琳王思雨李春王长海
Owner SHIHEZI UNIVERSITY
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