Macroporous micro-carrier with liver cell specificity as well as preparation method and applications thereof
A technology of microcarriers and spheres, applied in the field of hepatocyte-specific macroporous microcarriers and its preparation, can solve the problems of inability to induce and improve the adhesion performance of hepatocytes, easy detachment of cells, and lack of hepatocyte specificity
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Embodiment 1
[0033] 1) Preparation of silk fibroin: boil 75g of raw silk in 2L of 5g / L sodium carbonate solution for 0.5 hour, repeat twice, then wash with a large amount of distilled water to remove sericin, dry at 60-70°C, Get silk fibroin. Dissolve an appropriate amount of silk fibroin in a ternary solution of calcium chloride / water / ethanol (molar ratio 1:8:2) at 80±2°C, dialyze in distilled water for 3 days at room temperature, and remove the salt and ethanol in the solution , filter to remove insoluble impurities, and prepare an aqueous solution of silk fibroin. Stir and concentrate the silk fibroin aqueous solution at 50±2°C and 50-60 rpm to obtain a silk fibroin solution with a concentration of about 7-10w / v%;
[0034] 2) Preparation of galactosylated chitosan (GC): Weigh 2.2g of chitosan, dissolve it in 30-40ml 2.0% acetic acid aqueous solution, add an appropriate amount of TEMED / HCl buffer solution (pH 4.7) to dissolve the shell Dilute the polysaccharide solution to 4w / v%, then ...
Embodiment 2
[0042] 1) Preparation of silk fibroin: boil 75g of raw silk in 4L of 5g / L sodium carbonate solution for 0.5 hours, repeat twice, then wash with a large amount of distilled water to remove sericin, dry at 60-70°C, Get silk fibroin. Dissolve an appropriate amount of silk fibroin in a ternary solution of calcium chloride / water / ethanol (molar ratio 1:8:2) at 80°C, and dialyze in distilled water for 3 days at room temperature to remove salt, ethanol and other substances in the solution. small molecules, and then filtered to remove insoluble impurities to prepare an aqueous solution of silk fibroin. Stir and concentrate the silk fibroin aqueous solution at 50±2°C and 50-60 rpm to obtain a silk fibroin solution with a concentration of about 7-10w / v%;
[0043] 2) Preparation of galactosylated chitosan (GC): take 1.1g chitosan, dissolve it in 15-20ml 2.0% acetic acid aqueous solution, and then add an appropriate amount of TEMED / HCl buffer solution (pH4.7) to dissolve Dilute the chito...
Embodiment 3
[0051] 1) Preparation of silk fibroin: boil 75g of raw silk in 2L of 5g / L sodium carbonate solution for 0.5 hour, repeat twice, then wash with a large amount of distilled water to remove sericin, dry at 60-70°C, Get silk fibroin. Dissolve an appropriate amount of silk fibroin in a ternary solution of calcium chloride / water / ethanol (molar ratio 1:8:2) at 80±2°C, dialyze in distilled water for 3 days at room temperature, and remove the salt and ethanol in the solution , filter to remove insoluble impurities, and prepare an aqueous solution of silk fibroin. Stir and concentrate the silk fibroin aqueous solution at 50±2°C and 50-60 rpm to obtain a silk fibroin solution with a concentration of about 7-10w / v%;
[0052] 2) Preparation of galactosylated chitosan (GC): take 2.2g chitosan, dissolve it in 30-40ml 2.0% acetic acid aqueous solution, and then add an appropriate amount of TEMED / HCl buffer solution (pH4.7) to dissolve Dilute the chitosan solution to 4w / v%, then add 0.14g NH...
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