Biomaterial scaffolds for controlled tissue growth
A biomaterial and tissue growth technology, applied in the field of implants and tissue scaffolds, can solve problems such as uncontrolled and unsuitable for tissue engineering scaffolds, and achieve the effect of optimizing the dissolution rate
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[0102] experiment
[0103] method
[0104] Conical Fiber Preparation
[0105] The composition ratio is 0.5(P 2 o 5 ):0.25(CaO):(Na 2 O) dissolvable glass fibers with an average diameter of 30-40 μm (dissolving time in distilled water 8-10 hrs; spacing of 170 μm, cut into 2.3×3.5 cm and placed on a cover glass, fixed with autoclave tape. Then the cover glass Put into small Coplin container, then this container fills the Tris buffer solution (pH7.4) of 7ml 1%TRITON x-100 (Sigma). Remove liquid with the rate of 800 μ l per hour from this vial. Remove by the dehydration of ascending alcohol The accumulated hydrogel (for 20, 30, 40, 50, 60 and 90%, 5 minutes × 2; for 96%, 10 minutes × 2; for 100%, 15 minutes × 2) was washed with HMDS ( Hexamethyldisilazide) washing ends.
[0106] electron microscopy
[0107] The slides were left to dry overnight before the glass fibers were gently removed from the coverslips and mounted on sputter-coated stubs using standard procedures. ...
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