Double membrane tissue patching material and preparation method thereof

A repair material and double-layer film technology, applied in medical science, prosthesis, etc., can solve the problems of unfavorable wound epidermis cells crawling and sealing the wound, and it is difficult to observe the infection of the wound, so as to repair malnutrition and infected wounds and immune rejection Good response and extended shelf life

Active Publication Date: 2009-02-11
SHAANXI RUISHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the commercialized double-layer membranous biomaterial has Integra (produced by Integra LifeSciences), which is an artificial skin with a double-layer matrix. The cortex is composed of a silicone membrane, which can control the evaporation of wound moisture. During the wound healing process, the dermis will combine with the wound. The silicone membrane of the epidermis can be removed after the dermis is healed, and then further autologous skin transplantation, the US FDA has approved Integra was approved to be used in the treatment of burns and scalds in 1996; its main disadvantage is that due to the non-degradable silicone film on its surface, it is not easy to observe the early infection of the wound, which is not conducive to the crawling of wound edge epidermal cells and the sealing of the wound. The silicone membrane still needs a second operation

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] Step 1). Acquisition of fibroblasts: Take the foreskin tissue after neonatal resection, wash it with phosphate buffer saline (PBS) after disinfection, remove the subcutaneous fat and muscle layer, cut the skin into strips, and use 4U / ml medium Protease solution for digestion for 2 hours; remove the epidermis, cut the dermal tissue into pieces, digest with 625U / ml collagenase solution for 2.5 hours, and harvest fibroblasts for culture; use cell factory or spinner bottle for the primary cultured fibroblasts Cultivation system, or bioreactor and other methods for expanded cultivation;

[0020] Step 2). Preparation of the double-layer decellularized small intestinal submucosa: rinse the fresh porcine jejunum with distilled water, cut into required fragments, soak and sterilize in an aqueous solution containing 0.2% peracetic acid and 8% ethanol for 1 hour, and use Rinse with PBS solution; mechanically scrape off the small intestinal mucosa, muscle layer and serosa, rinse wi...

Embodiment 2

[0026] Step 1). Acquisition of fibroblasts: refer to the literature Dermal matrix as a carrier for in vivo delivery of human adipose-derived stem cells. Biomaterials. 2008 Apr; 29(10): 1431-42. It has been proved that adipose-derived mesenchymal stem cells can Differentiate into fibroblasts. Take healthy adipose tissue discarded during the operation, wash it with phosphate buffered saline (PBS) after disinfection, cut up the adipose tissue, digest it with 625U / ml collagenase solution for 150 minutes, and inoculate the harvested adipose-derived mesenchymal stem cells into culture bottles Cultivate; and then use cell factory, or spinner bottle culture system, or bioreactor method to expand culture;

[0027] Step 2). Decellularized dermis preparation: rinse the fresh pig skin with distilled water, remove the epidermis and subcutaneous tissue layer mechanically, cut into 0.4mm thick, 5cm 2 Size, soak and disinfect in an aqueous solution containing 0.2% (v / v) peracetic acid and 5%...

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Abstract

The invention discloses a double-layer membranous tissue repair material and a preparation method thereof, wherein, a cell-free membranous biological derivative material is used as a surface layer, and a fibroblast is compounded in the interior of a biological support material to form a substrate, and then the surface layer and the substrate are combined in a chimeric way to form the double-layer membranous tissue repair material; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as prevent the infection of the surface of wound, thus being beneficial to epitheliosis and epithelial growth; the substrate can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; compared with the existing products, the tissue repair material has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface of wound is healed, reducing hyperplasia of scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the invention has wide material resources and simple production method; the double-layer membranous tissue repair material prepared is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biomaterials, and in particular relates to a double-layer membranous tissue repair material and a preparation method thereof. Background technique [0002] Skin is an important tissue organ that covers and protects the body surface. Defects and abnormalities of the skin are often caused by inflammation, ulcers, trauma, burns, postoperative tumors, and congenital deformities. For exposed skin wounds, gauze is traditionally used the most. Gauze is mostly processed from cotton, burlap and linen, and is an inert material that does not directly promote wound healing. With the in-depth research on wound healing, it is realized that not only the wound surface must be covered, but also the wound must be helped to heal. Biomaterials composed of extracellular matrix are one of the ideal products, and their main components include collagen, non-collagenous glycoproteins, elastic fibers, glycosa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/26A61L27/18A61L27/20A61L27/22A61L27/24A61L27/60
Inventor 王爱军
Owner SHAANXI RUISHENG BIOTECH
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