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Decellularized flap as well as preparation method and application thereof

A decellularization and skin flap technology, which is applied in the fields of clinical medicine, biomedicine and regenerative medicine, can solve the problems of cumbersome operation steps and long time-consuming, and achieve simple operation steps, short preparation time, good cell compatibility and tissue compatibility effect

Inactive Publication Date: 2017-05-24
浙江保尔曼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The first object of the present invention is to provide a method for preparing a cell-free skin flap, so as to alleviate the technical problems of cumbersome and time-consuming operation steps in the prior art

Method used

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  • Decellularized flap as well as preparation method and application thereof
  • Decellularized flap as well as preparation method and application thereof
  • Decellularized flap as well as preparation method and application thereof

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Experimental program
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preparation example Construction

[0036] A method for preparing a decellularized skin flap provided by the invention comprises the following steps:

[0037] The perforator skin flap of the artery was taken, and heparin solution, Triton X-100 solution and SDS solution were perfused sequentially; the perforated skin flap after perfusion was dried, sterilized and kept aseptically.

[0038] In the present invention, the artery is the superficial epigastric artery.

[0039] The superficial epigastric artery perforator flap does not include the rectus abdominis muscle and its anterior sheath, but only contains skin, subcutaneous fat, and vascular pedicles. It has the advantages of traditional flaps and avoids complications. Since the decellularized skin flap is to remove the cellular components in the normal skin flap, the extracellular matrix structure of the full-thickness skin and subcutaneous tissue is preserved, and it can be used to repair wounds with full-thickness skin and subcutaneous tissue defects.

[00...

Embodiment 1

[0065] Embodiment 1 heparin PBS solution concentration

Embodiment 1－1

[0067] Perfuse the perforator flap obtained in the experiment: heparin PBS solution with a concentration of 15 U / mL.

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Abstract

The invention provides a decellularized flap as well as a preparation method and application thereof, and relates to the technical field of clinical medicine, biomedicine and regenerative medicine. The preparation method of the decellularized flap, which is provided by the invention, comprises the following steps of infusing a heparin solution, a Triton X-100 solution and an SDS ((Sodium Dodecyl Sulfate) solution in sequence, and then carrying out drying, sterilization and storage treatments. Operation steps are simple; the preparation is short in consumed time. The decellularized flap provided by the invention has no an immunological rejection reaction, does not relate to hypertoxic and harmful chemical substances, and further has favorable cytocompatibility and histocompatibility. Through the application of the decellularized flap provided by the invention to the repair of the wound surface of a defect and to scientific research, the restoration of defects of a full-thickness skin and a subcutaneous tissue can be realized; the healing quality is good.

Description

technical field [0001] The invention relates to the technical fields of clinical medicine, biomedicine and regenerative medicine, in particular to a decellularized skin flap and its preparation method and application. Background technique [0002] Burns, trauma, surgery, chronic ulcers, etc. cause skin defects, and the body with large areas of defects cannot repair its appearance and function by itself. Defective wounds need to be covered with skin grafts, and autologous skin flaps are commonly used in clinical practice. Take healthy skin from other areas of the patient's body (donor area) to repair the defect wound (recipient area). This method can effectively cover the skin defect wound, but often results in damage to the donor site. With the development of tissue engineering, a large number of artificial skin substitutes have emerged. The existing skin substitutes are mainly divided into 4 categories: [0003] One is decellularized dermis: allogeneic and heterogeneous...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/36A61L27/50
Inventor 梅劲高伟阳余雅玲杨良慧柴益民魏鹏陈川周乐斌
Owner 浙江保尔曼生物科技有限公司
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