44 results about "Long term toxicity" patented technology

The invention belongs to the technological field of the Chinese medicine, particularly relating to an extraction and separation processes of the active ingredients with the active function of treating the chronic glomerulonephritis. The mullein indican is the active compound researched and developed for many years in our research institute; the mullein indican is extracted, separated and refined from the digitalis leaf and is an active compound with the function of treating the chronic glomerulonephritis. The invention is the research result of many years held and developed by the Chinese Medicine Research Department of the Chinese Medicine Research Institute of China and is supported by the special fund of the Central Institute of the National Science and Technology Department; now the compound has been approved by the State Food and Drug Administration Bureau as the novel drug and the mechanism research of the treatment of the chronic nephritis has been completed; the pre-clinical research work includes the main pharmacodynamic experiment, the production technology experiment (including the industrialized extraction and separation processes), the quality control standard, the experiment of the affecting factors of the quality, the experiment of the long-term stability, the acute toxicity of the rat, the experiment of the long-term toxicity, the teratogenic and mutagenic experiment, the general pharmacological experiment, the pharmacokinetics experiment, and so on. The digitalis leaf is mainly produced in the Huaiqing area of the Henan province, and is also grown wild and cultivated in the Shaanxi, Hebei and other places of China.

The invention relates to a process for preparing a Guifudihuang dripping pill. The Guifudihuang dripping pill comprises the following medicines: 1-2 parts of Cinnamomum cassia, 1-2 parts of Radix Aconiti Lateralis Preparata (processed), 8-16 parts of prepared rehmannia, 4-8 parts of Fructus Corni, 3-6 parts of tree peony bark, 4-8 parts of Chinese yam, 3-6 parts of Indian Buead, and 3-6 parts of oriental waterplantain rhizome. The process of the invention has the following advantages: the taking amount of the dripping pill, which is greatly reduced, is reduced by 50% than the taking amount of original Guifudihuang capsules because all the medicines are extracted with different solvents according to main effective components and physical and chemical properties of each medicine of above composition, and a D101 macroporous adsorption resin column is adopted for separation, purification and impurity removal; and a fat and water soluble matrix which is adopted for dispersion allows the time limit of disintegration and dissolution to be substantially shortened, extracts to be dispersed in the matrix, the absorption to be good, the biological utilization degree to be improved, and the curative effect to be improved. Animal experiments show that the Guifudihuang dripping pill has substantial improvement effects on symptoms of kidney and qi yang deficiency, long-term toxicity tests show that the Guifudihuang dripping pill is safe in clinical applications; and compared with quality control standards of Guifudihuang pills and the Guifudihuang capsules, the quality control standard of the Guifudihuang dripping pill is greatly improved.

The invention discloses charge reversal type polymeric micelles, charge reversal type polymeric drug-loading micelles and their preparation methods. Alkaline polyamino acid containing protecting groups and acidic polyamino acid containing protecting groups are subjected to crosslinking reaction under the crosslinking action of BACy crosslinker so as to obtain a crosslinked polymer; the crosslinkedpolymer is hydrolyzed such that the protecting groups are removed separately from the alkaline polyamino acid and the acidic polyamino acid in the crosslinked polymer so as to obtain the charge reversible type polymeric micelles with three-dimensional mesh structure formed by crosslinking of positively charged polyamino acid and negatively charged polyamino acid. The charge reversal type polymeric micelles and the charge reversal type polymeric drug-loading micelles have good structural stability, good biocompatibility, low long-term toxicity and the like, also have reduction response and pHresponse, are available for long-term accumulation and high permeation in tumor cells, and provide a higher release rate of drugs.

The invention discloses a medicine compound for protecting liver and alleviating temulence and a preparation method thereof. The preparation method comprises the following steps: taking penthorum chinense pursh, smoked plum, radix puerariae, hovenia acerba, and the like, as main active ingredients; cutting the penthorum chinense pursh into small sections of 2-3mm, adding water for decocting and extracting for three times, gradually reducing the dosage of added water at each time, and merging the filtrates obtained at three times, thereby obtaining a penthorum chinense pursh extracting solution; crushing smoked plum, radix puerariae, hovenia acerba, rhizoma phragmitis, ginseng leaves, plantains and hawthorns into rough powder, adding water for decocting and extracting for three times, gradually reducing the dosage of added water at each time, and merging the filtrates obtained at three times, thereby obtaining a seven-medicine extracting solution; mixing the penthorum chinense pursh extracting solution with the seven-medicine extracting solution, and concentrating till forming a thick paste; and finally preparing a target compound capsule. An animal experiment and a clinical application prove that the medicine compound provided by the invention has excellent effect of protecting liver and alleviating temulence; all the raw materials are prepared at proportion, so that a synergistic effect is obtained; the preparation technique is simple; the mechanical production is conveniently performed; an animal acute and long-term toxicity experiments prove that the medicine compound is free from toxic side effect; the safety is high; and a new selection is supplied to clinical application.

The invention discloses a Chinese medicinal ointment with antibacterial and antipruritic effects and a preparation method thereof. The Chinese medicinal ointment comprises the following raw materials: amur corktree bark, lightyellow sophora root, common ducksmeat herb, aloe, argy wormwood leaf, fineleaf schizonepeta herb, borneol and the like. The invention also discloses a preparation method forthe Chinese medicinal ointment, which comprises the following steps: preparing a water phase from the amur corktree bark, the lightyellow sophora root, the common ducksmeat herb and the aloe; collecting volatile oil from the argy wormwood leaf and the fineleaf schizonepeta herb; grinding the borneol into fine powder; preparing an oil phase from cetyl alcohol, vaseline and glycerin; and preparing the ointment by normal working procedures. When the Chinese medicinal ointment is prepared, a water decoction heat filter method and a steam distillation method are respectively adopted to make effective medicaments give full play. When used, the Chinese medicinal ointment has the characteristics that the medicaments are directly acted on disease positions; and the response is quick. The ointment of the invention has good antibacterial, anti-inflammatory and antipruritic effects and is in a dose-response relationship. The long-term toxicity research proves that the Chinese medicinal ointment is safe.

The present invention provides a coconut juice composition, which comprises coconut juice and traditional Chinese medicine extracts, wherein the traditional Chinese medicine extracts comprise a sharpleaf galangal fruit extract, a milkvetch root extract, a medlar extract, a glossy privet fruit extract, a creeping liriope extract and a licorice root extract. The present invention further provides a preparation method for the coconut juice composition. According to the present invention, the sharpleaf galangal fruit extract, the milkvetch root extract, the medlar extract, the glossy privet fruit extract, the creeping liriope extract and the licorice root extract are added, wherein sharpleaf galangal fruit provides effects of spleen and kidney warming, qi strengthening and spermatorrhea treating, milkvetch root provides effects of qi reinforcing, superficial defence strengthening, diuresis and tissue regeneration promotion, medlar provides effects of liver and kidney invigoration, essence strengthening and eyesight improving, creeping liriope provides effects of yin nourishing, body fluid regeneration, lung moisturizing and heart-fire clearing, glossy privet fruit provides effects of liver and kidney invigoration, hair blackening and eyesight improving, licorice root extract provides effects of middle warmer regulating, liver qi stasis treatment, spleen invigorating, qi benefiting, lung moisturizing, detoxication and drug blending, and the coconut juice composition prepared through mixing the traditional Chinese medicine extracts and the coconut juice has effects of body nourishing, immunity enhancing, beautifying and face nourishing. The results show that the coconut juice composition has functions of anti-oxidation, no acute toxicity and no long-term toxicity, wherein immune function of liver cancer H22-bearing mice can be enhanced with the coconut juice composition.

The invention discloses a compound platycodon root hard capsule for treating dyspepsia and a preparation process thereof. The compound platycodon root hard capsule comprises platycodon root oil and peppermint oil with treatment effective quantity, wherein the menthol content of the peppermint oil is greater than 25 percent. The preparation process comprises the following process steps of: mixing medicinal liquid; encapsulating the mixed medicinal liquid into hard capsule shells which can be used medicinally; and sleeving the hard capsule shells and sealing to prepare a hard capsule preparation for encapsulating liquid. Preparation stability tests and animal long-term toxicity tests prove that when the hard capsule preparation is taken in the preparation form, the hard capsule preparation has an exact curative effect and high bioavailability, and is safe and reliable. The preparation process has the characteristics of scientificity, reasonableness, time saving, high efficiency, stable quality, low cost and the like.

The invention discloses a traditional Chinese medicine composition for relieving cough and asthma. The traditional Chinese medicine composition comprises the following medicinal raw materials by weight: 4-15 parts of ephedra, 4-15 parts of purple perilla leaf, 4-15 parts of lumbricus, 4-15 parts of folium eriobotryae, 2-10 parts of perillaseed, 3-12 parts of cicada ecdysis, 3-12 parts of radix peucedani, 4-15 parts of burdock, 3-12 parts of schisandra chinensis and 4-15 parts of almond. On the basis of the conventional traditional Chinese medicine preparation method, the traditional Chinese medicine composition is prepared into a common preparation by directly or indirectly adding pharmaceutically acceptable auxiliary materials during conventional processing. The traditional Chinese medicine composition has the obvious efficacies of relieving asthma and cough; acute toxicity and long-term toxicity tests prove that no toxic or side effect is caused.

The invention discloses a composition for preventing and treating flu as well as a preparation method and an application thereof. The composition comprises the following components in parts by mass: 10 to 20 parts of bupleurum, 10 to 15 parts of libanotis buchtormensis, 10 to 15 parts of artemisia rupestris and 10 to 15 parts of Taiqiang. With the aid of 'Qinling forest herb resource protection and development and utilization technology research thereof' of a special scientific research item in the public forestry welfare industry of State Forestry Bureau, the anti-flu Chinese medicinal preparation consisting of four special Chinese medicinal herbs in Mount Taibai of Qinling and using the bupleurum as a monarch is prepared. Pharmacodynamic animal experiments, acute toxicity experiments and long-term toxicity experiments for pharmacodynamic treatment effect evaluation and toxicity safety evaluation verify that the composition has a remarkable treatment effect of treating flu virus infection, particularly cold caused by H1N1 flu viruses, and is safe and nontoxic.

The invention relates to a pharmaceutic preparation for preventing and treating arrhythmia and application thereof. The pharmaceutic preparation is prepared from common macrocarpium fruit, salviae miltiorrhizae, saffron, milkvetch roots, turmeric root tubers, rhizoma chuanxiong, common cephalanoplos herb, lotus seeds, fructus arctii, alpine swertia, cinnamon, corn stigmas, fructus schizandrae, licorice roots, polygonumaviculareL., gold silkworms, pear peels, honeysuckle flowers, cape jasmine fruit, Huai wheat, immature bitter oranges and poria cocos. According to the prescription, materials are selected scientifically, the pharmaceutic preparation prepared through the preparation method has the prominent function of preventing or treating the arrhythmia, and the pharmaceutic preparation can prominently prolong the occurrence time of rat arrhythmia ventricular beat, ventricular tachycardia and ventricular fibrillation caused by aconitine and reduce animal mortality. By means of acute toxicity tests and long term toxicity tests, the toxic and side effects are low, the safety of drug use can be obviously improved, and the pharmaceutic preparation for preventing and treating the arrhythmia and the application thereof are suitable for further application and promotion in clinic.

The invention relates to a polypeptide with anticoagulant activity and a method for screening the polypeptide with anticoagulant activity through phage display technology, which belongs to the technical field of research and development and application of anticoagulant drugs; the invention adopts the construction of protein expression vector, expression and purification of target protein , verification of the target protein, phage display panning for bioactive peptides specifically binding to the target protein, in vivo and in vitro detection of the anticoagulant effect of the polypeptide, and toxicity experiments, and finally screened the polypeptides with anticoagulant activity; these polypeptides are based on Heparin-binding epidermal growth factor is used as the target molecule, and after three rounds of panning by phage display technology, heparin sodium is used as the active ingredient to elute the phage specifically binding to the target molecule. The polypeptide can be used to prepare anticoagulant drugs; the polypeptide of the present invention The sequence is short, easy to synthesize and achieve large-scale production, and has no significant short-term and long-term toxicity to mice in vivo, showing that it has important application value in the development of anticoagulant drugs.

The invention discloses a traditional Chinese medicine composition for relieving cough and asthma. The traditional Chinese medicine composition comprises the following medicinal raw materials by weight: 4-15 parts of ephedra, 4-15 parts of purple perilla leaf, 4-15 parts of lumbricus, 4-15 parts of folium eriobotryae, 2-10 parts of perillaseed, 3-12 parts of cicada ecdysis, 3-12 parts of radix peucedani, 4-15 parts of burdock, 3-12 parts of schisandra chinensis and 4-15 parts of almond. On the basis of the conventional traditional Chinese medicine preparation method, the traditional Chinese medicine composition is prepared into a common preparation by directly or indirectly adding pharmaceutically acceptable auxiliary materials during conventional processing. The traditional Chinese medicine composition has the obvious efficacies of relieving asthma and cough; acute toxicity and long-term toxicity tests prove that no toxic or side effect is caused.

The invention discloses a composition for preventing and treating flu as well as a preparation method and an application thereof. The composition comprises the following components in parts by mass: 10 to 20 parts of bupleurum, 10 to 15 parts of libanotis buchtormensis, 10 to 15 parts of artemisia rupestris and 10 to 15 parts of Taiqiang. With the aid of 'Qinling forest herb resource protection and development and utilization technology research thereof' of a special scientific research item in the public forestry welfare industry of State Forestry Bureau, the anti-flu Chinese medicinal preparation consisting of four special Chinese medicinal herbs in Mount Taibai of Qinling and using the bupleurum as a monarch is prepared. Pharmacodynamic animal experiments, acute toxicity experiments and long-term toxicity experiments for pharmacodynamic treatment effect evaluation and toxicity safety evaluation verify that the composition has a remarkable treatment effect of treating flu virus infection, particularly cold caused by H1N1 flu viruses, and is safe and nontoxic.

The invention relates to a new process method for preparing Guifudihuang capsule. The Guifudihuang capsule comprises 1-2 parts of cassia bark, 1-2 parts of prepared common monkshood daughter root (prepared), 8-16 parts of prepared rehmannia root, 4-8 parts of wine-prepared fructus corni, 3-6 parts of tree peony root-bark, 4-8 parts of common yam rhizome, 3-6 parts of tuckahoe and 3-6 parts of oriental waterplantain tuber. The process method provided by the present invention has the following advantages that: according to main active ingredients and physical and chemical properties of various drugs, different solvents for the drugs are adopted to carry out extraction; a D101 macroporous adsorption resin column is adopted to carry out separation, purification and impurity removing, such that the taking amount of the capsule is substantially reduced, wherein the taking amount of the capsule provided by the present invention is reduced by 85% than the taking amount of the capsule prepared through the existing process method; animal experiments show that: the capsule provided by the present invention provides a significant treatment effect for kidney yang deficiency syndrome; long-term toxicity tests show that: the clinical application of the capsule provided by the present invention is safe, the quality control standard of the capsule provided by the present invention is substantially improved compared to the taking amount of the capsule prepared through the existing process.