44 results about "Long term toxicity" patented technology

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.

The invention discloses a vibrio qinghaiensis Q67 based long-term microplate toxicity analyzing method of environmental pollutants. The long-term microplate toxicity analyzing method is established by using the vibrio qinghaiensis Q67 as a tested organism and introducing time factors into the toxicity concentration-effect relationship of the traditional compound on the basis of a microplate toxicity analyzing method. The vibrio qinghaiensis Q67 based long-term microplate toxicity analyzing method of the environmental pollutants is characterized by adopting a specific microplate design and sampling scheme and a microplate culture medium suitable for the growth of the vibrio qinghaiensis Q67, measuring the long-term toxicity effect of the environmental pollutants on the vibrio qinghaiensis Q67 and repeatedly measures the toxicity of the same pollutant through multiple plates to ensure the statistical significance of an experimental result. By measuring the long-term toxicity of a part of the environmental pollutants on the vibrio qinghaiensis Q67, compared with short-term toxicity, the long-term toxicity of the tested environmental pollutants is discovered to be obviously higher than the short-term toxicity of the tested environmental pollutants, wherein the difference of the long-term toxicity and the short-term toxicity of antibiotics is most significant. The invention can actually reflect the toxicity of a site compound having specific action on compounds and more reasonably evaluate the toxicity of the pollutants.

The invention belongs to the technological field of the Chinese medicine, particularly relating to an extraction and separation processes of the active ingredients with the active function of treating the chronic glomerulonephritis. The mullein indican is the active compound researched and developed for many years in our research institute; the mullein indican is extracted, separated and refined from the digitalis leaf and is an active compound with the function of treating the chronic glomerulonephritis. The invention is the research result of many years held and developed by the Chinese Medicine Research Department of the Chinese Medicine Research Institute of China and is supported by the special fund of the Central Institute of the National Science and Technology Department; now the compound has been approved by the State Food and Drug Administration Bureau as the novel drug and the mechanism research of the treatment of the chronic nephritis has been completed; the pre-clinical research work includes the main pharmacodynamic experiment, the production technology experiment (including the industrialized extraction and separation processes), the quality control standard, the experiment of the affecting factors of the quality, the experiment of the long-term stability, the acute toxicity of the rat, the experiment of the long-term toxicity, the teratogenic and mutagenic experiment, the general pharmacological experiment, the pharmacokinetics experiment, and so on. The digitalis leaf is mainly produced in the Huaiqing area of the Henan province, and is also grown wild and cultivated in the Shaanxi, Hebei and other places of China.

The invention relates to a Chinese herbal medicine for treating respiratory tract diseases, comprising the proportional extracts of the following raw medicine: 10-30 portion of tangerine peel, 10-15 portion of balloonflower, 10-30 portion of rhizoma typhonii flagelliformis, 50-150 portion of loquat leaf, 5-15 portion of tussilago farfara, 5-15 portion of fritillaria cirrhosa, 10-30 portion of glehnia littoralis, 2-8 portion of schisandra chinensis, 10-30 portion of 0.1% aqua armeniacae, 0-1 portion of almond oil, and 0.1-0.3 portion of menthol; suitable excipients are added to make various suitable formulations applied to cough condition initiated by respiratory tract diseases; wherein, there are 306 cases in treatment group, 119 cases in control group. The analysis of the total therapeutic effect shows that clinical efficacy rate of the Chinese herbal medicine for treating respiratory tract diseases is 97.1%, the cure rate 44.8%, validity 18.3%, efficiency 34.0%;in the control group clinical efficacy rate is 78.2%, the cure rate 26.9%, validity 11.8%, efficiency 39.5%. The Chinese herbal medicine for treating respiratory tract diseases has the advantages: obviously the therapeutic effect is better than that of the compared tangerine peel productive cough soft extract; the long-term toxicity study shows that the Chinese herbal medicine for treating respiratory tract diseases is safe and reliable.

Disclosed is lentinus edodes fermented pure protein with anti-tumor activity as well as an extracting method and a preparation thereof. The protein is obtained through fermentation, salting-out, dialysis, drying and column chromatography of mycelia of lentinus edodes C91-3; the protein is light brown or earthy yellow powder which can be dissolved in water and has specific fragrance, the pH value of aqueous solution is 5.0 to 8.0, the molecular weight is 1 KD to 90 KD, and the protein contains 18 types of amino acids and various proteins. The experiment proves that in the anti-tumor experiment in vitro, the tumor restraining rate of the lentinus edodes fermented pure protein LFP91-3-A to H22 and S180 in 72 hours can achieve 59.93 percent and 80.13 percent; lentinus edodes fermented pure protein LFP91-3-A has an inducing effect on H22 cell telomerase of which the activity is obviously restrained and apoptotic. The tumor restraining rate of lentinus edodes fermented pure protein LFP91-3-B to S180 can achieve 76.7 percent; the experiment proves that the strain fermented pure protein has relatively strong effect of directly killing tumor cells. The lentinus edodes fermented pure protein has no toxic side effect through animal acute toxicity experiments and long-term toxicity experiments.

The invention relates to a Chinese medicinal compound preparation for resisting virus and treating flu and cold. The preparation consists of the following raw materials by weight: 300 to 600g of honeysuckle, 200 to 400g of indigowoad leaf, 200 to 400g of weeping forsythia capsule, 200 to 400g of Chinese thorowax root, 200 to 400g of baical skullcap root, 200 to 400g of platycodon grandiflorum, and 1 to 10g of menthol. Pharmacodynamic tests prove that honeysuckle and Chinese thorowax root anti-flu capsules have the effects of reducing fever, diminishing inflammation and relieving cough, wherein the effect of reducing fever is more remarkable. Acute toxicity tests prove that: the safe dosage of the Chinese medicinal composition is equivalent to 371.2 times that of maximum clinical dosage of a human. Long-term toxicity test researching results prove that: remarkable toxic changes or delayed toxicity reactions caused by medicament are not found. The Chinese medicinal compound preparation has the functions of clearing heat and removing toxicity, coursing wind and dissipating heat, and ventilating lung and relieving cough, and has the advantages of convenient use, controllable quality, definite drug effect and safety and reliability.

The invention relates to a process for preparing a Guifudihuang dripping pill. The Guifudihuang dripping pill comprises the following medicines: 1-2 parts of Cinnamomum cassia, 1-2 parts of Radix Aconiti Lateralis Preparata (processed), 8-16 parts of prepared rehmannia, 4-8 parts of Fructus Corni, 3-6 parts of tree peony bark, 4-8 parts of Chinese yam, 3-6 parts of Indian Buead, and 3-6 parts of oriental waterplantain rhizome. The process of the invention has the following advantages: the taking amount of the dripping pill, which is greatly reduced, is reduced by 50% than the taking amount of original Guifudihuang capsules because all the medicines are extracted with different solvents according to main effective components and physical and chemical properties of each medicine of above composition, and a D101 macroporous adsorption resin column is adopted for separation, purification and impurity removal; and a fat and water soluble matrix which is adopted for dispersion allows the time limit of disintegration and dissolution to be substantially shortened, extracts to be dispersed in the matrix, the absorption to be good, the biological utilization degree to be improved, and the curative effect to be improved. Animal experiments show that the Guifudihuang dripping pill has substantial improvement effects on symptoms of kidney and qi yang deficiency, long-term toxicity tests show that the Guifudihuang dripping pill is safe in clinical applications; and compared with quality control standards of Guifudihuang pills and the Guifudihuang capsules, the quality control standard of the Guifudihuang dripping pill is greatly improved.

The invention relates to polypeptides with anticoagulation activity and a method for screening polypeptides with anticoagulation activity by a phage display technique, belonging to the technical field of development, research and application of anticoagulant drugs. The screening method comprises the following steps: construction of protein expression vector, expression and purification of target protein, verification of target protein, elutriation of bioactive peptide capable of specifically binding target protein by phage display, polypeptide anticoagulation action in-vivo / in-vitro detection, toxicity experimentation and the like, thereby finally obtaining the polypeptides with anticoagulation activity. The polypeptides are prepared by the following steps: by using a heparin-binding epidermal growth factor as a target molecule, carrying out elutriation three times by using a phage display technique, and eluting the specific binding target molecule phage by using heparin sodium as an effective component. The polypeptides can be used for preparing anticoagulant drugs. The polypeptides have shorter sequence, are easy for synthesis, can easily implement large-scale production, and have no obvious short-term and long-term toxicity for mice in vivo, thereby having important application value in the aspect of development and research of anticoagulant drugs.

The invention discloses charge reversal type polymeric micelles, charge reversal type polymeric drug-loading micelles and their preparation methods. Alkaline polyamino acid containing protecting groups and acidic polyamino acid containing protecting groups are subjected to crosslinking reaction under the crosslinking action of BACy crosslinker so as to obtain a crosslinked polymer; the crosslinkedpolymer is hydrolyzed such that the protecting groups are removed separately from the alkaline polyamino acid and the acidic polyamino acid in the crosslinked polymer so as to obtain the charge reversible type polymeric micelles with three-dimensional mesh structure formed by crosslinking of positively charged polyamino acid and negatively charged polyamino acid. The charge reversal type polymeric micelles and the charge reversal type polymeric drug-loading micelles have good structural stability, good biocompatibility, low long-term toxicity and the like, also have reduction response and pHresponse, are available for long-term accumulation and high permeation in tumor cells, and provide a higher release rate of drugs.

The invention provides a magnetic nanometer diagnosis and treatment agent and a preparation method and application thereof. The magnetic nanometer diagnosis and treatment agent comprises polypyrrole nanoparticles doped with ferroferric oxide nanoparticles and hyaluronic acid adsorbed to the surfaces of the polypyrrole nanoparticles. The nanometer diagnosis and treatment agent is small in grain size, good in penetrability, uniform in grain size, easy to purify, free of obvious cytotoxicity, good in biocompatibility, good in long circulating effect and low in long-term toxicity, and negative charges exist on the surfaces of the particles; an outstanding T2 relaxation enhancement effect is achieved, the linear relation of the T2 relaxation time reciprocal and the iron concentration change within the low concentration range is good, and the nanometer diagnosis and treatment agent has photo-acoustic imaging capability, is an ideal nanometer diagnosis and treatment agent for preparing multi-mode images and is remarkable in photo-thermal and magnetic-thermal treatment effect. The preparation method is simple, conditions are mild, cost is low, and application and popularization are easy.

The invention discloses a medicine compound for protecting liver and alleviating temulence and a preparation method thereof. The preparation method comprises the following steps: taking penthorum chinense pursh, smoked plum, radix puerariae, hovenia acerba, and the like, as main active ingredients; cutting the penthorum chinense pursh into small sections of 2-3mm, adding water for decocting and extracting for three times, gradually reducing the dosage of added water at each time, and merging the filtrates obtained at three times, thereby obtaining a penthorum chinense pursh extracting solution; crushing smoked plum, radix puerariae, hovenia acerba, rhizoma phragmitis, ginseng leaves, plantains and hawthorns into rough powder, adding water for decocting and extracting for three times, gradually reducing the dosage of added water at each time, and merging the filtrates obtained at three times, thereby obtaining a seven-medicine extracting solution; mixing the penthorum chinense pursh extracting solution with the seven-medicine extracting solution, and concentrating till forming a thick paste; and finally preparing a target compound capsule. An animal experiment and a clinical application prove that the medicine compound provided by the invention has excellent effect of protecting liver and alleviating temulence; all the raw materials are prepared at proportion, so that a synergistic effect is obtained; the preparation technique is simple; the mechanical production is conveniently performed; an animal acute and long-term toxicity experiments prove that the medicine compound is free from toxic side effect; the safety is high; and a new selection is supplied to clinical application.

The invention discloses a Chinese medicinal composition for preventing and treating diabetes and a preparation method thereof. The Chinese medicinal composition consists of the following components in parts by weight: 10-20 parts of vervain, 1-2 parts of typhonium giganteum, 10-20 parts of barley root, 10-20 parts of coptis root, 10-20 parts of black fungus, 10-20 parts of white fungus, 10-20 parts of sticky rice, 10-20 parts of barley rice, 10-20 parts of buckwheat, 5-50 parts of honey and 50-100 parts of vinegar. Natural animal and plant medicinal materials are taken as raw material sources, and diabetics syndrome and nephrotic syndrome are treated by using the comprehensive function of various Chinese medicinal components. As proved by acute toxicity test and long-term toxicity test, the Chinese medicinal composition is nontoxic to human body and good in medicinal effect and has the effects of improving the hypercoagulable state, improving blood fat, reducing blood sugar, dissolving thrombi, dredging microcirculation and improving the immunity of organisms.

The invention relates to a new process method for preparing Guifudihuang capsule. The Guifudihuang capsule comprises 1-2 parts of cassia bark, 1-2 parts of prepared common monkshood daughter root (prepared), 8-16 parts of prepared rehmannia root, 4-8 parts of wine-prepared fructus corni, 3-6 parts of tree peony root-bark, 4-8 parts of common yam rhizome, 3-6 parts of tuckahoe and 3-6 parts of oriental waterplantain tuber. The process method provided by the present invention has the following advantages that: according to main active ingredients and physical and chemical properties of various drugs, different solvents for the drugs are adopted to carry out extraction; a D101 macroporous adsorption resin column is adopted to carry out separation, purification and impurity removing, such that the taking amount of the capsule is substantially reduced, wherein the taking amount of the capsule provided by the present invention is reduced by 85% than the taking amount of the capsule prepared through the existing process method; animal experiments show that: the capsule provided by the present invention provides a significant treatment effect for kidney yang deficiency syndrome; long-term toxicity tests show that: the clinical application of the capsule provided by the present invention is safe, the quality control standard of the capsule provided by the present invention is substantially improved compared to the taking amount of the capsule prepared through the existing process.

The invention discloses a stomach medicine which is characterized by comprising the following Chinese medical herbs in parts by weight: 80-120 parts of rubber, 120-180 parts of dragon's blood, 80-120 parts of ruddle, 150-250 parts of calcined ark shell and 120-180 parts of Fritillaria hupehenis Hsiao et K.C.Hsia. The traditional formula is integrated and improved and the Chinese medicinal herbs are matched with mineral medicines, thus the stomach medicine has the advantages of special formula, accurate treatment effect, low cost, no toxic and side effects, and smooth medicine properties, and has the effects of soothing liver and harmonizing stomach, promoting qi circulation and relieving pain, relieving the stasis and refreshing mind, regulating the flow of vital energy and removing obstruction toit, reducing phlegm, promoting qi circulation and removing obstruction in the collateral, dredging the Shao-Yang channels, warming without drying, and tonifying without causing stagnation; and the stomach medicine has ulcer cure rate of 93 percent in 8 weeks for the peptic ulcer and disappear rate of 95 percent for the chronic gastritis, remarkable treatment effect on the chronic atrophic gastritis which can not be effectively treated at present, and no toxic and side effects through chute and long term toxicity tests.

The invention provides magnetic nano pharmaceutical complex and a preparation method application thereof. The magnetic nano pharmaceutical complex comprises ferroferric oxide nanoparticle doped polypyrrole nano-carrier, pharmaceutical DAPT carried on the surface of the polypyrrole nano-carrier, and hyaluronic acid adsorbed to the surface of the polypyrrole nano-carrier that carries the pharmaceutical DAPT. The magnetic nano pharmaceutical complex has high ability to carry the pharmaceutical DAPT, good stability and small particle size, has good penetrating capacity and uniform particle size, has significant magneto-thermal slow control, has long-cycle effect in vivo, has no evident cytotoxicity, has low long-term toxicity and good biocompatibility, has significant killing effect on tumor stem cells, and has better killing effect particularly in connection with magneto-thermal therapy; the preparation method of the complex is simple, has mild conditions and low cost and is easy to popularize and apply.

Belonging to the field of medical technologies, the invention discloses application of Tupistra chinensis in preparation of antitumor drugs. MTT assay and flow cytometry find that Tupistra chinensis can block the growth cycle of an S-180 cell, inhibit its proliferation, and induce tumor cell apoptosis. EMSA and real-time fluorescent quantitative PCR find that Tupistra chinensis can reduce the expression of NF-kB and inhibit the activity of NF-kB, and has an inhibitory effect on the S-180 cell; Tupistra chinensis can reduce the expression of NF-kB in an S-180 cell inoculated tumor-bearing mouse model tumor body tissue, also can inhibit the activity of NF-kB in the tumor-bearing mouse model tumor body tissue, and has a tumor growth inhibiting effect. Acute toxicity tests and the long-term toxicity tests prove that Tupistra chinensis has no influence on the hepatorenal functions and blood glucose level of Kunming mice and has damage on organs of Kunming mice. The invention shows that Tupistra chinensis has a safe and effective antitumor effect, and can be used for preparing antitumor drugs.

The invention discloses a Chinese medicinal ointment with antibacterial and antipruritic effects and a preparation method thereof. The Chinese medicinal ointment comprises the following raw materials: amur corktree bark, lightyellow sophora root, common ducksmeat herb, aloe, argy wormwood leaf, fineleaf schizonepeta herb, borneol and the like. The invention also discloses a preparation method forthe Chinese medicinal ointment, which comprises the following steps: preparing a water phase from the amur corktree bark, the lightyellow sophora root, the common ducksmeat herb and the aloe; collecting volatile oil from the argy wormwood leaf and the fineleaf schizonepeta herb; grinding the borneol into fine powder; preparing an oil phase from cetyl alcohol, vaseline and glycerin; and preparing the ointment by normal working procedures. When the Chinese medicinal ointment is prepared, a water decoction heat filter method and a steam distillation method are respectively adopted to make effective medicaments give full play. When used, the Chinese medicinal ointment has the characteristics that the medicaments are directly acted on disease positions; and the response is quick. The ointment of the invention has good antibacterial, anti-inflammatory and antipruritic effects and is in a dose-response relationship. The long-term toxicity research proves that the Chinese medicinal ointment is safe.

The present invention provides a coconut juice composition, which comprises coconut juice and traditional Chinese medicine extracts, wherein the traditional Chinese medicine extracts comprise a sharpleaf galangal fruit extract, a milkvetch root extract, a medlar extract, a glossy privet fruit extract, a creeping liriope extract and a licorice root extract. The present invention further provides a preparation method for the coconut juice composition. According to the present invention, the sharpleaf galangal fruit extract, the milkvetch root extract, the medlar extract, the glossy privet fruit extract, the creeping liriope extract and the licorice root extract are added, wherein sharpleaf galangal fruit provides effects of spleen and kidney warming, qi strengthening and spermatorrhea treating, milkvetch root provides effects of qi reinforcing, superficial defence strengthening, diuresis and tissue regeneration promotion, medlar provides effects of liver and kidney invigoration, essence strengthening and eyesight improving, creeping liriope provides effects of yin nourishing, body fluid regeneration, lung moisturizing and heart-fire clearing, glossy privet fruit provides effects of liver and kidney invigoration, hair blackening and eyesight improving, licorice root extract provides effects of middle warmer regulating, liver qi stasis treatment, spleen invigorating, qi benefiting, lung moisturizing, detoxication and drug blending, and the coconut juice composition prepared through mixing the traditional Chinese medicine extracts and the coconut juice has effects of body nourishing, immunity enhancing, beautifying and face nourishing. The results show that the coconut juice composition has functions of anti-oxidation, no acute toxicity and no long-term toxicity, wherein immune function of liver cancer H22-bearing mice can be enhanced with the coconut juice composition.

The invention discloses a compound platycodon root hard capsule for treating dyspepsia and a preparation process thereof. The compound platycodon root hard capsule comprises platycodon root oil and peppermint oil with treatment effective quantity, wherein the menthol content of the peppermint oil is greater than 25 percent. The preparation process comprises the following process steps of: mixing medicinal liquid; encapsulating the mixed medicinal liquid into hard capsule shells which can be used medicinally; and sleeving the hard capsule shells and sealing to prepare a hard capsule preparation for encapsulating liquid. Preparation stability tests and animal long-term toxicity tests prove that when the hard capsule preparation is taken in the preparation form, the hard capsule preparation has an exact curative effect and high bioavailability, and is safe and reliable. The preparation process has the characteristics of scientificity, reasonableness, time saving, high efficiency, stable quality, low cost and the like.

The invention discloses a traditional Chinese medicine composition for relieving cough and asthma. The traditional Chinese medicine composition comprises the following medicinal raw materials by weight: 4-15 parts of ephedra, 4-15 parts of purple perilla leaf, 4-15 parts of lumbricus, 4-15 parts of folium eriobotryae, 2-10 parts of perillaseed, 3-12 parts of cicada ecdysis, 3-12 parts of radix peucedani, 4-15 parts of burdock, 3-12 parts of schisandra chinensis and 4-15 parts of almond. On the basis of the conventional traditional Chinese medicine preparation method, the traditional Chinese medicine composition is prepared into a common preparation by directly or indirectly adding pharmaceutically acceptable auxiliary materials during conventional processing. The traditional Chinese medicine composition has the obvious efficacies of relieving asthma and cough; acute toxicity and long-term toxicity tests prove that no toxic or side effect is caused.

The invention belongs to the field of traditional Chinese medicines, and in particular relates to a novel application of genipin-1-[beta]-D-gentiobioside and a composition thereof. The genipin-1-[beta]-D-gentiobioside and the composition thereof can take a significant effect on treating a model rat with cerebral ischemia-reperfusion injury, obviously reduce behavioral scores of the model rat, significantly diminish the area of a brain slice infarction area of the model rat and reduce TNF-[alpha] and 1L-6 contents in serum of the model rat; an obvious therapeutic effect can be taken on a Bend.3 cell ischemic injury model which is cultured in vitro, the survival rate of injured cells can be significantly improved and a cell death-protection rate is positively related to a drug concentration; and the contents of tumor necrosis factors (TNF-[alpha]) and interleukin 6 (1L-6) in supernatant liquid of a Bend.3 cell hypoxia / reoxygenation model which is cultured in vitro can be obviously reduced. Moreover, based upon an acute toxicity test in mice and a long-term toxicity in rats, it indicates that the genipin-1-[beta]-D-gentiobioside and the composition thereof are free from toxic and side reactions.

The invention discloses a Chinese medicinal preparation for treating dyspepsia, which is characterized by consisting of the following components in part by weight: 2 to 20 parts of costustoot, 2 to 20 parts of jasminum amplexicaule, 2 to 20 parts of bitter orange, 1 to 10 parts of raw rhubarb, 1 to 10 parts of rhubarb, 2 to 20 parts of officinal magnolia bark, 0.1 to 0.8 part of Chinese knotweed herb, 1 to 10 parts of pomegranate rind and 10 to 60 parts of Chinese date. Preparation stability tests and animal long-term toxicity tests prove that the Chinese medicinal preparation has definite curative effect and high bioavailability, and is safe and reliable; and the preparation process has the characteristics of high efficiency, stable quality, low cost and the like, and is scientific, reasonable and time-saving.

The invention discloses a composition for preventing and treating flu as well as a preparation method and an application thereof. The composition comprises the following components in parts by mass: 10 to 20 parts of bupleurum, 10 to 15 parts of libanotis buchtormensis, 10 to 15 parts of artemisia rupestris and 10 to 15 parts of Taiqiang. With the aid of 'Qinling forest herb resource protection and development and utilization technology research thereof' of a special scientific research item in the public forestry welfare industry of State Forestry Bureau, the anti-flu Chinese medicinal preparation consisting of four special Chinese medicinal herbs in Mount Taibai of Qinling and using the bupleurum as a monarch is prepared. Pharmacodynamic animal experiments, acute toxicity experiments and long-term toxicity experiments for pharmacodynamic treatment effect evaluation and toxicity safety evaluation verify that the composition has a remarkable treatment effect of treating flu virus infection, particularly cold caused by H1N1 flu viruses, and is safe and nontoxic.

The invention relates to a pharmaceutic preparation for preventing and treating arrhythmia and application thereof. The pharmaceutic preparation is prepared from common macrocarpium fruit, salviae miltiorrhizae, saffron, milkvetch roots, turmeric root tubers, rhizoma chuanxiong, common cephalanoplos herb, lotus seeds, fructus arctii, alpine swertia, cinnamon, corn stigmas, fructus schizandrae, licorice roots, polygonumaviculareL., gold silkworms, pear peels, honeysuckle flowers, cape jasmine fruit, Huai wheat, immature bitter oranges and poria cocos. According to the prescription, materials are selected scientifically, the pharmaceutic preparation prepared through the preparation method has the prominent function of preventing or treating the arrhythmia, and the pharmaceutic preparation can prominently prolong the occurrence time of rat arrhythmia ventricular beat, ventricular tachycardia and ventricular fibrillation caused by aconitine and reduce animal mortality. By means of acute toxicity tests and long term toxicity tests, the toxic and side effects are low, the safety of drug use can be obviously improved, and the pharmaceutic preparation for preventing and treating the arrhythmia and the application thereof are suitable for further application and promotion in clinic.

The invention discloses a traditional Chinese medicine broiler feed additive. The traditional Chinese medicine broiler feed additive is prepared from following components by weight: 20-30 parts of radix astragali, 10-20 parts of radix acanthopanacis semticosi, 10-20 parts of fructus crataegi, 10-16 parts of folium artemisiae argyi, 15-25 parts of pine needle meal and 5-15 parts of cortex magnoliaeofficinalis. A preparation method comprises the steps as follows: the components are weighed in the raw material ratio, impurities are removed, and the components are washed and dried; the componentsare smashed, and water is added for soaking the components; the components are decocted and filtered, and a filtrate is collected; detection is performed again, filtering is performed, and a filtrateis collected; the two filtrates are combined and concentrated, and the traditional Chinese medicine broiler feed additive is obtained. The acute toxicity test proves that the additive is safe and non-toxic, and the long-term toxicity test proves that the feed additive is safe to use in a feed range of 75 g / kg; and the feed additive has a good effect for improvement of production performance and immune resistance of healthy broilers, remarkably improves production performance and immune resistance of sick broilers and is better than astragalus polysaccharides.

The invention relates to granules for treating infantile fever and respiratory tract infection and application thereof. The granules comprise taraxacum officinale, poria cocos, radix isatidis, ramulus mori, shaddock peel, fructus arctii, aristolochia debilis, asparagus cochinchinensis, terminalia chebula, scutellaria baicalensis, fructus forsythiae, radix angelicae dahuricae, pericarpium citri reticulatae, endothelium corneum gigeriae galli, crataegus pinnatifida, cornus officinalis, platycodon grandiflorus, areca catechu, folium eriobotryae, semen raphani, semen descurainiae, periostracum cicadae, aquilaria sinensis, spica schizonepetae, andrographis laxiflora and fructus aurantii. By aiming at physiological properties of children, the traditional Chinese medicinal materials with different drug properties are adopted and combined scientifically, so that the granules have stronger functions of relieving fever and dispelling cold, enhancing gastrointestinal tract digestion and improving immunity of children patients; besides, drug toxicity and long-term toxicity test results found that the granules are safe, reliable, little in toxic and side effect, short in cure period on the aspect of curative effect, convenient in administration, easy to produce and suitable for large-scale industrial production, and can create a higher social value and economic benefit.

The invention relates to a polypeptide with anticoagulant activity and a method for screening the polypeptide with anticoagulant activity through phage display technology, which belongs to the technical field of research and development and application of anticoagulant drugs; the invention adopts the construction of protein expression vector, expression and purification of target protein , verification of the target protein, phage display panning for bioactive peptides specifically binding to the target protein, in vivo and in vitro detection of the anticoagulant effect of the polypeptide, and toxicity experiments, and finally screened the polypeptides with anticoagulant activity; these polypeptides are based on Heparin-binding epidermal growth factor is used as the target molecule, and after three rounds of panning by phage display technology, heparin sodium is used as the active ingredient to elute the phage specifically binding to the target molecule. The polypeptide can be used to prepare anticoagulant drugs; the polypeptide of the present invention The sequence is short, easy to synthesize and achieve large-scale production, and has no significant short-term and long-term toxicity to mice in vivo, showing that it has important application value in the development of anticoagulant drugs.

The invention discloses a composition for preventing and treating flu as well as a preparation method and an application thereof. The composition comprises the following components in parts by mass: 10 to 20 parts of bupleurum, 10 to 15 parts of libanotis buchtormensis, 10 to 15 parts of artemisia rupestris and 10 to 15 parts of Taiqiang. With the aid of 'Qinling forest herb resource protection and development and utilization technology research thereof' of a special scientific research item in the public forestry welfare industry of State Forestry Bureau, the anti-flu Chinese medicinal preparation consisting of four special Chinese medicinal herbs in Mount Taibai of Qinling and using the bupleurum as a monarch is prepared. Pharmacodynamic animal experiments, acute toxicity experiments and long-term toxicity experiments for pharmacodynamic treatment effect evaluation and toxicity safety evaluation verify that the composition has a remarkable treatment effect of treating flu virus infection, particularly cold caused by H1N1 flu viruses, and is safe and nontoxic.