Application of Tupistra chinensis in preparation of antitumor drugs

Abstract

Belonging to the field of medical technologies, the invention discloses application of Tupistra chinensis in preparation of antitumor drugs. MTT assay and flow cytometry find that Tupistra chinensis can block the growth cycle of an S-180 cell, inhibit its proliferation, and induce tumor cell apoptosis. EMSA and real-time fluorescent quantitative PCR find that Tupistra chinensis can reduce the expression of NF-kB and inhibit the activity of NF-kB, and has an inhibitory effect on the S-180 cell; Tupistra chinensis can reduce the expression of NF-kB in an S-180 cell inoculated tumor-bearing mouse model tumor body tissue, also can inhibit the activity of NF-kB in the tumor-bearing mouse model tumor body tissue, and has a tumor growth inhibiting effect. Acute toxicity tests and the long-term toxicity tests prove that Tupistra chinensis has no influence on the hepatorenal functions and blood glucose level of Kunming mice and has damage on organs of Kunming mice. The invention shows that Tupistra chinensis has a safe and effective antitumor effect, and can be used for preparing antitumor drugs.

Description

technical field

[0001] The invention belongs to the technical field of medicine, and relates to the application of open arrows in the preparation of antitumor drugs. Background technique

[0002] Tumor, heart disease and cerebrovascular disease are the three major diseases that endanger human health and life in the 21st century. The morbidity and mortality of malignant tumors are increasing year by year, and the prevention and treatment of tumors is also an important topic of research at home and abroad in recent years. Through a large number of long-term clinical practice, it is known that traditional Chinese herbal medicine has its unique curative effect in anti-malignant tumors. It usually acts on multiple links of tumor occurrence and development. Improve the clinical symptoms and signs of tumor patients, reduce adverse reactions after radiotherapy and chemotherapy, improve patient immunity and prolong life. In clinical treatment, radiotherapy, chemotherapy and surgery...

Examples

Embodiment 1

[0021] Embodiment 1 Open arrow drug preparation

[0022] Weigh 0.5kg of Kaijianjian decoction pieces (Shennongjia production area, Guoyang County Yuanhetang Traditional Chinese Medicine Decoction Pieces Co., Ltd.), first wash the Kaikaijian decoction pieces twice with tap water, then soak them in tap water for 30 minutes, replace with 1.5L of distilled water and boil After 40-50 minutes, pour the obtained solution into a clean beaker prepared in advance, and gradually place it until it cools down, add 750mL of distilled water to the open arrow decoction pieces and boil for 40-50 minutes, then pour the solution into the same beaker to cool, Pour off the dregs of the drug, put the solution of the open arrow drug collected twice into the medicine tank, decoct and concentrate to 250mL, decoct twice to obtain a solution with a concentration of 2.0g / mL, filter it twice with sterile gauze after cooling, and separate The bottle is stored at 4°C. It can be diluted to 0.04g / mL and 1.0g / ...

Embodiment 2

[0023] Embodiment 2 MTT method detects the influence of open arrow on S-180 cell proliferation

[0024] Take the S-180 cells in the logarithmic growth phase and use the trypan blue staining method to measure the cell viability: dilute the cell suspension appropriately, mix it with 0.4% trypan blue solution at 9:1 (volume ratio), and take a little solution Drop into a clean counting plate, count the number of living cells and dead cells respectively, according to the colorless and transparent color of the living cells, and the dead cells are stained in obvious blue to calculate the cell viability: the rate of living cells (%) = the number of living cells / ( The number of viable cells + the number of dead cells) × 100%.

[0025] Select the S-180 cells with a viable cell rate > 96% and count them with a cell counting board, count the number of cells according to the number of cells / mL = the total number of cells in the four major grids / 4×10000, and dilute the cells to 4×10 accor...

Embodiment 3

[0031] Example 3 Establishment of tumor-bearing mouse model

[0032] Feed the mice adaptively for 4 days, and the mice with good growth status will be tested in the next step; take the S-180 cells in the logarithmic growth phase, count them with a hemocytometer, and dilute them to 2×10 with the culture medium. 5 / mL cell suspension, according to the pre-experimental results (KM mice and BALB / c-nu mice were intraperitoneally injected with cell suspension for 3, 5, 7, and 10 days without ascites growth), choose direct intradermal inoculation to establish tumor models . The preparation before the test is the same as above, the inoculation site is the left back armpit and the right back back; when inoculating, first use 75% alcohol for local routine disinfection, gently press the skin with the thumb and index finger of the left hand to make it taut, between the two fingers, use a The syringe needle of the cell suspension enters the superficial layer of the skin, then lifts up and...

technical field

[0001] The invention belongs to the technical field of medicine, and relates to the application of open arrows in the preparation of antitumor drugs. Background technique

[0002] Tumor, heart disease and cerebrovascular disease are the three major diseases that endanger human health and life in the 21st century. The morbidity and mortality of malignant tumors are increasing year by year, and the prevention and treatment of tumors is also an important topic of research at home and abroad in recent years. Through a large number of long-term clinical practice, it is known that traditional Chinese herbal medicine has its unique curative effect in anti-malignant tumors. It usually acts on multiple links of tumor occurrence and development. Improve the clinical symptoms and signs of tumor patients, reduce adverse reactions after radiotherapy and chemotherapy, improve patient immunity and prolong life. In clinical treatment, radiotherapy, chemotherapy and surgery...

Examples

Embodiment 1

[0021] Embodiment 1 Open arrow drug preparation

[0022] Weigh 0.5kg of Kaijianjian decoction pieces (Shennongjia production area, Guoyang County Yuanhetang Traditional Chinese Medicine Decoction Pieces Co., Ltd.), first wash the Kaikaijian decoction pieces twice with tap water, then soak them in tap water for 30 minutes, replace with 1.5L of distilled water and boil After 40-50 minutes, pour the obtained solution into a clean beaker prepared in advance, and gradually place it until it cools down, add 750mL of distilled water to the open arrow decoction pieces and boil for 40-50 minutes, then pour the solution into the same beaker to cool, Pour off the dregs of the drug, put the solution of the open arrow drug collected twice into the medicine tank, decoct and concentrate to 250mL, decoct twice to obtain a solution with a concentration of 2.0g / mL, filter it twice with sterile gauze after cooling, and separate The bottle is stored at 4°C. It can be diluted to 0.04g / mL and 1.0g / ...

Embodiment 2

[0023] Embodiment 2 MTT method detects the influence of open arrow on S-180 cell proliferation

[0024] Take the S-180 cells in the logarithmic growth phase and use the trypan blue staining method to measure the cell viability: dilute the cell suspension appropriately, mix it with 0.4% trypan blue solution at 9:1 (volume ratio), and take a little solution Drop into a clean counting plate, count the number of living cells and dead cells respectively, according to the colorless and transparent color of the living cells, and the dead cells are stained in obvious blue to calculate the cell viability: the rate of living cells (%) = the number of living cells / ( The number of viable cells + the number of dead cells) × 100%.

[0025] Select the S-180 cells with a viable cell rate > 96% and count them with a cell counting board, count the number of cells according to the number of cells / mL = the total number of cells in the four major grids / 4×10000, and dilute the cells to 4×10 accor...

Embodiment 3

[0031] Example 3 Establishment of tumor-bearing mouse model

[0032] Feed the mice adaptively for 4 days, and the mice with good growth status will be tested in the next step; take the S-180 cells in the logarithmic growth phase, count them with a hemocytometer, and dilute them to 2×10 with the culture medium. 5 / mL cell suspension, according to the pre-experimental results (KM mice and BALB / c-nu mice were intraperitoneally injected with cell suspension for 3, 5, 7, and 10 days without ascites growth), choose direct intradermal inoculation to establish tumor models . The preparation before the test is the same as above, the inoculation site is the left back armpit and the right back back; when inoculating, first use 75% alcohol for local routine disinfection, gently press the skin with the thumb and index finger of the left hand to make it taut, between the two fingers, use a The syringe needle of the cell suspension enters the superficial layer of the skin, then lifts up and...

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