Method for increasing efficiency of tissue culture of Medicago sativa L.

A technique of tissue culture and alfalfa, applied in the field of plant biology, can solve problems such as insufficient callus formation and differentiation, long induction time of alfalfa tissue culture, explant contamination, etc., to retain differentiation ability and increase callus induction rate , The effect of low seed pollution rate
CN102499094AInactive Publication Date: 2012-06-20GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
Publication Date
2012-06-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of plant biology, in particular relates to a method of tissue culture of Medicago sativa L. By way of developing a novel tissue culture technical method, the invention focuses on improvement and innovation of two technical nodes (seed disinfection and callus formation and differentiation) in culture steps. With adoption of a mercury bichloride and hydrogen peroxide compound disinfection method, the invention realizes the purposes of thorough disinfection effect and low pollution rate and minimum damage to seeds. Finally, the invention solves the problem of severe pollution to current explants. Meanwhile, a method of TDZ and 6-BA is adopted, thereby, the callus induction rate and the formation rate are remarkably increased, the callus quality is improved, the differentiation capacity of the callus is reserved to a maximum extent, the formation of embryoid sprouts is promoted so that the novel technical method of the tissue culture of the Medicago sativa L. which is efficient, saves time, and has low toxicity and low browning, is obtained.
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Description

technical field

[0001] The invention belongs to the field of plant biotechnology, in particular to a method for tissue culture of alfalfa (Medicago sativa L.). The present invention develops a new tissue culture technology method, focusing on improving and innovating the two major technical nodes of seed disinfection and callus formation and differentiation in the culture steps, so as to obtain a new type of alfalfa tissue culture with high efficiency, low toxicity and low browning. Technical method. Background technique

[0002] Alfalfa (Medicago sativa L.) is an important leguminous forage, known as the "king of forage" because of its high nutritional value and good palatability. Tissue culture of alfalfa mainly refers to the technical method of using the isolated organs of alfalfa (cotyledon, radicle, etc.) to carry out plant regeneration through artificial cultivation. The method is of great significance for the reproduction and preservation of the precious genetic mat...

Claims

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