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Tissue-engineered bone cartilage composite scaffold and preparation method thereof

A tissue-engineered bone and composite scaffold technology, used in tissue regeneration, medical science, prosthesis, etc., to achieve the effects of good biocompatibility, biocompatibility, degradability, and enhanced mechanical properties

Inactive Publication Date: 2016-03-30
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can effectively solve the problem of tissue engineering osteochondral composite scaffold preparation

Method used

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  • Tissue-engineered bone cartilage composite scaffold and preparation method thereof
  • Tissue-engineered bone cartilage composite scaffold and preparation method thereof
  • Tissue-engineered bone cartilage composite scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Accurately weigh 1.00g of polyε-caprolactone, add it into 5mL of chloroform, and prepare a 20w / v% solution, stir and dissolve evenly; accurately weigh 2.00g of polyethylene glycol, add 50ngBMP-2 into 5mL of distilled water, and prepare 40w / v% solution, magnetic stirring to dissolve evenly. Inject into 5mL dry syringes respectively, the syringes are fixed in the card slot of the micro-injection pump, and the output end of the high-voltage DC power supply is fixed on the coaxial nozzle (the inner diameter of the outer needle is 1.1mm, the outer diameter of the inner needle is 0.6mm, and the inner diameter of the inner needle is 0.3 mm), the outer axis is polyε-caprolactone solution, and the flow rate is set at 0.5mL / h. The inner axis is polyethylene glycol solution, the flow rate is set to 0.1mL / h, and the DC high voltage is applied to 20kV. The high-speed rotating drum is used as the receiver (the speed is 1200r / min), and the distance between the coaxial nozzle and the r...

Embodiment 2

[0049] Accurately weigh 1.00g of polyε-caprolactone, add it into 5mL of chloroform, and prepare a 20w / v% solution, stir and dissolve evenly; accurately weigh 2.00g of polyethylene glycol, add 50ngBMP-2 into 5mL of distilled water, and prepare 40w / v% solution, magnetic stirring to dissolve evenly. Inject into 5mL dry syringes respectively, the syringes are fixed in the card slot of the micro-injection pump, and the output end of the high-voltage DC power supply is fixed on the coaxial nozzle (the inner diameter of the outer needle is 1.1mm, the outer diameter of the inner needle is 0.6mm, and the inner diameter of the inner needle is 0.3 mm), the outer axis is polyε-caprolactone solution, and the flow rate is set to 0.5mL / h; the inner axis is polyethylene glycol solution, the flow rate is set to 0.1mL / h, and the DC high voltage is 20kV, which is rotated at a high speed. The roller is used as a receiver (rotating speed is 1200r / min), and the distance between the coaxial nozzle a...

Embodiment 3

[0057] Accurately weigh 1.00g of polyε-caprolactone, add it into 5mL of chloroform, and prepare a 20w / v% solution, stir and dissolve evenly; accurately weigh 2.00g of polyethylene glycol, add 50ngBMP-2 into 5mL of distilled water, and prepare 40w / v% solution, magnetic stirring to dissolve evenly. Inject into 5mL dry syringes respectively, the syringes are fixed in the card slot of the micro-injection pump, and the output end of the high-voltage DC power supply is fixed on the coaxial nozzle (the inner diameter of the outer needle is 1.1mm, the outer diameter of the inner needle is 0.6mm, and the inner diameter of the inner needle is 0.3 mm), the outer axis is polyε-caprolactone solution, and the flow rate is set to 0.5mL / h; the inner axis is polyethylene glycol solution, the flow rate is set to 0.1mL / h, and the DC high voltage is 20kV, which is rotated at a high speed. The roller is used as a receiver (rotating speed is 1200r / min), and the distance between the coaxial nozzle a...

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Abstract

The invention discloses a tissue-engineered bone cartilage composite scaffold and a preparation method thereof, belonging to the technical field of biomaterials. The scaffold is of a multilayer integrated structure and comprises a cartilage tissue scaffold layer, a cartilage tissue calcified layer, a porous cell isolation membrane and a bone tissue scaffold layer. The cartilage tissue scaffold layer is inoculated with cartilage cells, and growth factors for promoting formation of cartilage are introduced to promote growth of cartilage cells. Main raw materials like heparan sulfate proteoglycan with good biocompatibility and degradability are selected and used and the method consisting of a cross-linking reaction, freeze drying, directional pore formation, compounding of multiple layers, overall integration and the like are carried out so as to obtain the functionalized multilayer integrated tissue-engineered bone cartilage composite scaffold with good mechanical properties. According to the invention, micro-nano hydroxyapatite crystal, degradable non-stoichiometric polyethylene glycol / poly(epsilon-caprolactone) nanometer coaxial short fiber, RGD-grafted heparan sulfate proteoglycan / oxidized sodium alginate and N-succinyl chitosan are compounded together to prepare the composite scaffold; and the composite scaffold is mainly used for restoration of full-thickness defects of articular cartilage and subchondral bone.

Description

technical field [0001] The invention belongs to the technical field of biomaterial engineering, and relates to the preparation technology of tissue engineering scaffold of human articular cartilage. Background technique [0002] At present, cartilage defects at the end of movable joints caused by trauma, infection, tumor, and degeneration are common orthopedic clinical diseases. Cartilage wear and damage to articular surfaces. Because articular cartilage has no blood vessels, nerves, and lymphatic systems, cartilage defects with a diameter of more than 2-4 mm can hardly completely repair themselves; and clinically, simple articular cartilage injuries are relatively rare, and more are accompanied by subchondral bone lesions, including those caused by trauma Defect of subchondral bone, necrosis of subchondral bone caused by osteochondritis, sclerosis and fatty change of subchondral bone caused by cartilage degeneration. Therefore, it is necessary to repair the subchondral bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/54A61L27/38
CPCA61L27/3817A61L27/3821A61L27/3847A61L27/3852A61L27/46A61L27/54A61L2300/252A61L2300/414A61L2300/602A61L2430/02A61L2430/06A61L2430/24C08L5/10C08L5/04C08L5/08
Inventor 汪建新陈太军白家繁王莹莹翁杰冯波鲁雄周绍兵
Owner SOUTHWEST JIAOTONG UNIV
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