9results about How to "Promote gene expression" patented technology

The invention discloses a plant cell nucleus extraction method and application, and belongs to the technical field of plant single cell sequencing. The method comprises the following steps: carrying out tissue immobilization on plant tissues by using precooled methanol; preparing a cell nucleus lysis solution, namely an NIB solution; cleaning and chopping the fixed tissue sample, and incubating the tissue sample in an NIB solution; filtering with a screen; centrifuging, discarding supernatant, and resuspending and precipitating the NIB solution to obtain a suspension; slowly dripping the suspension into a Percoll solution, and carrying out density gradient centrifugation to purify cell nucleuses. The invention provides a high-applicability and high-quality cell nucleus extraction method which is suitable for cryopreserved or fresh tissue samples, higher cell nucleus yield and mRNA capture rate are obtained, and the data quality of single cell sequencing is improved.

The application discloses a sludge resource utilization method and application thereof. The sludge resource utilization method comprises the following steps: performing electro-fermentation treatment on sludge to be fermented in an anode chamber of an electrolytic cell; and the anode electrode in the anode chamber comprises a carbon-based material modified by titanium oxide. The anode electrode of the electrolytic cell comprises the carbon-based material modified by titanium oxide, the hydrophilicity of the surface of activated carbon particles can be significantly improved by modifying the carbon material by titanium oxide, the biocompatibility and electrocatalytic activity of the material are improved, the growth of microorganisms is facilitated, the anaerobic fermentation speed of bacteria can be improved, the electro-fermentation process speed and treatment effect are optimized and improved, and the effective recovery of elements such as nitrogen, iron and phosphorus contained in the sludge is realized, so that the application prospect is good.

The present invention discloses an anti-immune stress composition for chickens, a preparation method and an application thereof. The composition is composed of the following components in parts by weight: 15 to 25 parts of lotus leaves, 15 to 25 parts of Platycladus orientalis leaves, 10 to 20 parts of licorice polysaccharide, 0.1 to 0.5 parts of vitamin E, and 0.05 to 0.5 parts of nano zinc oxide or paeonol-capped nano zinc oxide. The anti-immune stress composition for chickens of the present invention, through the addition of traditional Chinese medicines with antioxidant, antibacterial, antiviral and liver protection properties, as well as nano zinc oxide and vitamin E, the components work synergistically, effectively improving the immune stress status of chickens, improving body metabolism, effectively improving the immunity of chickens, increasing the feed intake of chickens under immune stress, stimulating the digestion and absorption function of the gastrointestinal tract of chickens, and improving the ability of chickens to resist immune stress.

A Chinese medicine composition, an extract, a preparation method and application thereof, cosmetics and medicines, belonging to the field of Chinese medicine. The Chinese medicine composition comprises: forsythia fruit, tribulus fruit, acanthopanax, polygonatum, immature bitter orange and ginseng. By using the mutual cooperation of specific raw materials, forsythia fruit, tribulus fruit, acanthopanax, polygonatum, immature bitter orange and ginseng, it has the ability to inhibit MMP-1 gene expression, promote TIMP-1 expression, inhibit the generation of reactive oxygen, promote the expression of NQO1 gene reduced by oxidative stress, inhibit NO generation and inhibit iNOS expression. Experiments show that it not only has antioxidant, anti-wrinkle and anti-inflammatory effects, but is also non-toxic, so it can be used in medicines, cosmetics and health products.

The invention provides a biological preparation for improving collagen expression of skin cells, and belongs to the technical field of skin medicine. The biological agent is prepared from grass carp roe polypeptide and water. The grass carp roe polypeptide with the molecular weight smaller than 1 kDa is obtained through liquid nitrogen freezing and smashing, supercritical CO2 degreasing, alkaline condition regulation and control and ultrasonic-assisted extraction. Experimental results show that the grass carp roe polypeptide prepared by the invention significantly promotes collagen gene expression, improves the hydroxyproline level in skin tissues in a D-galactose induced aging mouse model, and effectively slows down skin aging. The grass carp roe polypeptide provided by the invention provides a new bioactive component for the fields of beauty and skin care, medical dressings and the like, and meanwhile, high value-added utilization of grass carp resources is realized.

This invention belongs to the field of agricultural biotechnology and discloses a lysozyme-nanomobionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire process. The system consists of a core engine, a biomimetic delivery system, and an enhancement module. It utilizes plant exosomes to simulate vesicle delivery, employing lysozyme to hydrolyze the cell walls of pathogens, releasing oligosaccharide signaling molecules, activating plant WAK receptors, and simultaneously initiating disease resistance, quality improvement, and yield enhancement pathways. Intelligent pH / ROS-responsive gating precisely releases components, while the nano-silicon photosynthetic engine enhances light efficiency, resulting in increased crop disease resistance, improved quality, and increased yield, while also promoting the green and low-carbon transformation of agriculture.

This invention relates to a three-dimensional porous bioactive scaffold, a vascularized bone organoid, its preparation method, and its applications, belonging to the field of tissue engineering technology. The bioactive scaffold includes a scaffold body, a Piezo1 channel agonist loaded on the scaffold body, and an Nrf2 agonist and / or Nrf2 expression vector loaded on the scaffold body; the scaffold body is a 3D-printed PMBG / β-TCP composite porous scaffold. The vascularized bone organoid uses the three-dimensional porous bioactive scaffold as a supporting framework and also includes cellular components, including co-cultured bone marrow mesenchymal stem cells and endothelial cells. Compared with existing technologies, this invention successfully constructs a novel vascularized bone organoid capable of targeting the Piezo1-Nrf2 signaling axis and possessing anti-endothelial aging and mechanical enhancement functions.

The invention provides application of Qixuekang in preparation of a medicine for treating renal fibrosis. Experimental research proves that the Qixuekang administration can remarkably recover the renal function of a mouse with renal fibrosis in senescence-accompanied chronic kidney diseases, reduce the mouse kidney mesenchymal fibrosis level and the levels of fibrosis markers alpha-SMA and Col1, recover the content of reductive glutathione in the mouse, improve the activity of superoxide dismutase and improve the curative effect of the kidney fibrosis in the senescence-accompanied chronic kidney diseases. Therefore, kidney fibrosis phenotypes and fibrosis-related biochemical indexes of mice are improved. Qi-blood Kang can remarkably restore kidney mesenchymal transformation related gene and protein expression, and kidney fibrosis phenotypes in chronic kidney diseases are improved by reducing related inflammatory media; qiwankang can significantly improve mitochondrial membrane potential, increase mitochondrial neogenesis related gene expression and reduce generation of active oxygen.

This invention provides a 3'-UTR element and its application in the production of target proteins. The 3'-UTR element comprises: (a) a nucleic acid molecule with a nucleotide sequence as shown in any of SEQ ID NO. 23, SEQ ID NO. 1-SEQ ID NO. 22, SEQ ID NO. 24-SEQ ID NO. 28, and SEQ ID NO. 37; (b) a nucleic acid molecule transcribed using the antisense strand of (a) as a template; or (c) a nucleic acid molecule derived from (a) or (b) by substitution, deletion, or addition of one or more nucleotides in the nucleotide sequence defined by (a) or (b) while retaining the biological function of its derived sequence. This element can stably and significantly enhance mRNA gene expression, providing a powerful tool for the development of mRNA vaccines and the optimization of gene expression in gene therapy.