73results about How to "Promote gene expression" patented technology

An implantable device for providing electrical stimulation of cervical vagus nerves for treatment of chronic cardiac dysfunction with leadless heart rate monitoring is provided. A stimulation therapy lead includes helical electrodes configured to conform to an outer diameter of a cervical vagus nerve sheath, and a set of connector pins electrically connected to the helical electrodes. A neurostimulator includes an electrical receptacle into which the connector pins are securely and electrically coupled. The neurostimulator also includes a pulse generator configured to therapeutically stimulate the vagus nerve through the helical electrodes in alternating cycles of stimuli application and stimuli inhibition that are tuned to both efferently activate the heart's intrinsic nervous system and afferently activate the patient's central reflexes by triggering bi-directional action potentials. Finally, the neurostimulator includes an integrated leadless heart rate sensor configured to sense heart rate and to record the sensed heart rate as data into a memory.

This present invention discloses nucleic acid compositions and methods that are useful for treating ischemic conditions in animals, particularly in mammals such as humans. Specifically, the invention discloses nucleic acid molecules comprising or encoding a sequence that modulates the stability of a transcript from a vascular endothelial growth factor gene, as well as pharmaceutical compositions containing such molecules, which arm useful for modulating angiogenesis or vascularization, especially in methods for treating ischemic conditions.

Described are new methods for promoting the expression of apolipoprotein A1 (APO A1) for increasing levels of HDL, and assays for screening and identifying compounds for regulating expression of the APO A1 protein.

The present invention provides reconstituted histone octamers with multiple modifications (e.g. acetylation of all histones and trimethylation of histone H3K4) assembled onto plasmids for increased transcription post-transfection using our unique bi-lamellar invaginated liposomes (BIVs) to more effectively recruit the transcriptional machinery of human cancer cells post-transfection and substantially increase the production of therapeutic gene products.

The present invention relates to the field of biotechnology and particularly relates to a method for preparing a soybean Aglycin family peptide by using acetic acid. The method for preparing the soybean Aglycin family peptide by using acetic acid comprises the following steps: subjecting raw materials to cleaning, soaking, germination, grinding and extraction, separating the slag from the slurry through a ceramic membrane and an organic membrane, and subjecting obtained materials to freeze-drying. According to the technical scheme of the invention, the acetic acid is extracted from soybeans, and the ceramic membrane and the organic membrane are combined to use, wherein the pore size of the ceramic membrane is 0.1 [mu]m and the pore size of the organic membrane is 1500 D to 6000 D. In this way, small protein and polypeptide compounds, with the molecular weight thereof between 1500 D to 6000 D, are obtained. The method is simple in operation, thoroughly in extraction effect, easy in filtration, short in production cycle, greatly improved in yield and greatly reduced in production cost. Therefore, the industrial production is easily realized. A produced Aglycin product is in the form of light yellow powders, and is good in taste and high in purity. The Aglycin product can be used as functional food for the adjuvant therapy of type 2 diabetes for preventing diabetes.

The invention discloses recombinant pseudomonas putida, which is expressed on the basis of DNA integration. The invention also discloses a construction method for the recombinant pseudomonas putida, and the application of the recombinant pseudomonas putida in phosphorus removal from wastewater. The method of the invention is simple, and obtained genetically engineered bacteria have high-efficiency phosphorus removal capacity.

Genes are expressed by culturing cells comprising a host chromosome comprising an integrated artificial chromosome comprising recombinant genes, under conditions whereby each recombinant gene is expressed copy number dependently and position independently. Deletions increase expression from recombinant gene(s) inserted into the artificial chromosome.

The present invention relates generally to the treatment and prevention of diseases characterized by excess cell proliferation and/or activation. In particular, the present invention provides compositions and methods to suppress the activation and/or proliferation of various cells. In some preferred embodiments, the present invention provides compositions and methods to suppress the activation and/or proliferation of mesenchymally derived cells (including, but not limited to hepatic stellate cells), as well as cells with abnormal growth characteristics. In some particularly preferred embodiments, the present invention provides compositions and methods to inhibit or eliminate fibrosis. In alternative preferred embodiments, the present invention provides compositions and methods to induce fibrosis. In still further embodiments, the present invention provides methods and compositions to treat and/or prevent cancer.

The invention relates to the administration of inhibitors of phosphodiesterase 1 (PDE1) for the treatment of diseases or disorders characterized by disruption of or damage to various cGMP/PKG mediated pathways. In one embodiment the invention relates to inhibitors of phosphodiesterase 1 (PDE1) for treatment of cardiovascular disease and related disorders, e.g., congestive heart disease, atherosclerosis, myocardial infarction, and stroke.

The invention discloses a loofah planting method. The loofah planting method comprises the following steps of (1) selecting and treating; (2) seedling seeding; (3) land arranging and fertilizer applying; (4) field planting; (5) harvesting. Loofah produced by the method has the advantages that the quality is high, the harvesting cycle is long, and the output is high. The method has the advantages that seeds are soaked into a seed treatment liquid, and are electrolyzed, so that the permeation property of seed coats is improved, cells in the seeds are promoted to suck water, and the quick maturing of incompletely matured seed embryos is promoted.

The present disclosure relates to microbial stem cell technology that enables a growing microbial culture to stably maintain two or more distinct cell types in a ratio that can be genetically programmed and/or dynamically controlled during cultivation. It is contemplated that embodiments described herein can be utilized to increase product yield in microbial fermentations and advanced engineering of biomaterials using genetically engineered microbial cells, among others.

The invention provides an interference method for bovine trim5alpha gene silencing, a siRNA sequence, a siRNA human lentivirus expression vector, a replication-defective human lentivirus and a human lentivirus infected MDBK (madin-darby bovine kidney) cell. RNAi and the siRNA human lentivirus expression vector effectively interfere with the expression of a bovine trim5alpha gene, and the gene expression of the human lentivirus vector in the MDBK cell is significantly improved. The siRNA provides the new technical support for research on bovine virus disease related gene functions and transgenic cattle breeding.

The invention provides a regulator for improving intestine health of juvenile fish of micropterus salmoides at a feed change stage and a preparation method and application of the regulator and belongsto the technical field of feed additives. The regulator comprises 5-30% of tea polyphenol, 5-20% of oxidized konjac glucomannan, 20-50% of linseed oil, 10-30% of inulin and 25-40% of a carrier. The preparation method specifically comprises the following steps: putting the linseed oil into a stainless steel reaction kettle, slowly adding the carrier, performing uniform stirring so as to obtain a linseed oil mixture, screening the linseed oil mixture by using an 80-100-mesh sieve, respectively adding the tea polyphenol, the oxidized konjac glucomannan and the inulin, performing uniform mixing and stirring, inspecting contents of components of the obtained mixture, and performing bottling and packaging. The obtained regulator is used in preparation of feeds for the juvenile fish of the micropterus salmoides at the feed change stage, intestine health of the juvenile fish of the micropterus salmoides at the feed change stage can be greatly improved, intestinal diseases can be reduced, anduse of antibiotics in the breeding process can be reduced.

The invention provides a culture medium and a method for establishing and maintaining early embryonic-like cells of mammals. The culture medium is used for culturing pluripotent stem cells (PSC) of mammals, has definite chemical components, contains a basic culture medium for culturing stem cells, and is supplemented with an S-adenosylhomocysteine hydrolase (SAH)/polycomb inhibition complex (PRC)/EZH2 inhibitor and an HDAC inhibitor. By means of the culture medium, primate (human and non-human) animal PSC can be converted into pre-implantation internal cell mass-like cells (ICLC) or 8-cell embryonic-like cells (8CLC).

A vector having a region encoding a cold shock protein gene mRNA-origin 5′-nontranslated region, characterized in that the 5′-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.