4results about How to "Promote gene expression" patented technology

This invention belongs to the field of agricultural biotechnology and discloses a lysozyme-nanomobionic system based on cell wall signal regulation and its application in improving the quality and efficiency of crops across the entire process. The system consists of a core engine, a biomimetic delivery system, and an enhancement module. It utilizes plant exosomes to simulate vesicle delivery, employing lysozyme to hydrolyze the cell walls of pathogens, releasing oligosaccharide signaling molecules, activating plant WAK receptors, and simultaneously initiating disease resistance, quality improvement, and yield enhancement pathways. Intelligent pH / ROS-responsive gating precisely releases components, while the nano-silicon photosynthetic engine enhances light efficiency, resulting in increased crop disease resistance, improved quality, and increased yield, while also promoting the green and low-carbon transformation of agriculture.

This invention relates to a three-dimensional porous bioactive scaffold, a vascularized bone organoid, its preparation method, and its applications, belonging to the field of tissue engineering technology. The bioactive scaffold includes a scaffold body, a Piezo1 channel agonist loaded on the scaffold body, and an Nrf2 agonist and / or Nrf2 expression vector loaded on the scaffold body; the scaffold body is a 3D-printed PMBG / β-TCP composite porous scaffold. The vascularized bone organoid uses the three-dimensional porous bioactive scaffold as a supporting framework and also includes cellular components, including co-cultured bone marrow mesenchymal stem cells and endothelial cells. Compared with existing technologies, this invention successfully constructs a novel vascularized bone organoid capable of targeting the Piezo1-Nrf2 signaling axis and possessing anti-endothelial aging and mechanical enhancement functions.

The invention provides application of Qixuekang in preparation of a medicine for treating renal fibrosis. Experimental research proves that the Qixuekang administration can remarkably recover the renal function of a mouse with renal fibrosis in senescence-accompanied chronic kidney diseases, reduce the mouse kidney mesenchymal fibrosis level and the levels of fibrosis markers alpha-SMA and Col1, recover the content of reductive glutathione in the mouse, improve the activity of superoxide dismutase and improve the curative effect of the kidney fibrosis in the senescence-accompanied chronic kidney diseases. Therefore, kidney fibrosis phenotypes and fibrosis-related biochemical indexes of mice are improved. Qi-blood Kang can remarkably restore kidney mesenchymal transformation related gene and protein expression, and kidney fibrosis phenotypes in chronic kidney diseases are improved by reducing related inflammatory media; qiwankang can significantly improve mitochondrial membrane potential, increase mitochondrial neogenesis related gene expression and reduce generation of active oxygen.

This invention provides a 3'-UTR element and its application in the production of target proteins. The 3'-UTR element comprises: (a) a nucleic acid molecule with a nucleotide sequence as shown in any of SEQ ID NO. 23, SEQ ID NO. 1-SEQ ID NO. 22, SEQ ID NO. 24-SEQ ID NO. 28, and SEQ ID NO. 37; (b) a nucleic acid molecule transcribed using the antisense strand of (a) as a template; or (c) a nucleic acid molecule derived from (a) or (b) by substitution, deletion, or addition of one or more nucleotides in the nucleotide sequence defined by (a) or (b) while retaining the biological function of its derived sequence. This element can stably and significantly enhance mRNA gene expression, providing a powerful tool for the development of mRNA vaccines and the optimization of gene expression in gene therapy.