Method for obtaining fusarium oxysporum resistant carnation clone

A technology of Fusarium oxysporum and Carnation, applied in the field of plant tissue culture, can solve problems such as accelerating the process of disease resistance breeding, and achieve the effects of saving field breeding cycles, high selection efficiency and convenient operation.

Pending Publication Date: 2021-03-26
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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Problems solved by technology

The in vitro screening technology of somaclonal variation can accelerate the ...

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  • Method for obtaining fusarium oxysporum resistant carnation clone
  • Method for obtaining fusarium oxysporum resistant carnation clone
  • Method for obtaining fusarium oxysporum resistant carnation clone

Examples

Experimental program
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Embodiment

[0030] The test material is the tissue culture seedling of the susceptible carnation multi-headed variety "Purple Butterfly". The cultivation method is as follows:

[0031] 1) Induction of carnation callus

[0032] The stem segment, leaf or shoot tip of carnation tissue culture seedlings are used to induce and obtain callus, and the medium formula for inducing callus is MS+Dicamaba 1mg L -1 +Sugar 30g / L+Hydrolyzed casein 300mg·L -1 , pH value 5.8; cultured for 30 days to obtain callus, after which it needs to be subcultured for 2-3 times to obtain yellow, loose callus with good embryogenicity.

[0033] The callus rate of stem segment, blade and shoot tip is more than 85%, and the callus rate of leaf is the highest, which is 94.44%. The induced callus grows better, and is a loose yellow callus ( figure 1 ).

[0034] 2) Callus suspension culture and EMS mutagenesis

[0035] Take 2 g of fresh yellow callus with loose embryonic quality, crush it gently with a sterilized gla...

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Abstract

The invention relates to a method for obtaining a fusarium oxysporum resistant carnation clone, belongs to the technical field of plant tissue culture, and aims at obtaining a fusarium oxysporum resistant breeding intermediate material by inducing calluses by utilizing carnation, mutagenizing by adopting ethyl methanesulfonate (EMS) and adding fusarium oxysporum toxin screening. According to the method, a large number of cells can be cultured and treated in a smaller space by utilizing a technology of adding pathogenic toxin into a culture medium to screen disease-resistant germplasm after clone mutation of the cells; direct induction and screening of mutants at the cellular level is an attempt of higher plant resistance breeding microbiotization; and the disease-resistant breeding periodcan be shortened, directional breeding is conducted, the selection efficiency is high, the effect is good, the field breeding period is shortened, and a new method is provided for disease-resistant breeding of carnation.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for obtaining carnation clones resistant to Fusarium oxysporum. Background technique [0002] Carnation (Dianthus caryophyllus L.), also known as Carnation, is a perennial herbaceous plant of the genus Dianthus Linn in the family Caryophyllaceae. 15% of world sales of fresh cut flowers. Since carnation was produced on a large scale in Yunnan in the late 1980s, carnation has accounted for nearly 1 / 3 of the fresh cut flowers in Yunnan and has become one of the main fresh cut flowers exported by Yunnan. According to statistics, in 2014, the production area of ​​cut flower carnation in Yunnan was 28,400 mu, with an annual output of more than 2 billion cut flowers. The main production areas are distributed in Kunming, Yuxi, Honghe, Chuxiong, Qujing and other places. [0003] The problem of pests and diseases has always been the primary factor restri...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张艺萍杨秀梅王丽花许凤瞿素萍蒋亚莲王继华张丽芳苏艳
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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