Mutagenesis method of seed lotus phenotypic variation mutant plant

Abstract

The invention discloses a mutagenesis method of a seed lotus phenotypic variation mutant plant. The mutagenesis method comprises the following steps of (1) shelling seed lotus seeds; (2) soaking the shelled lotus seeds in clear water to accelerate germination, and then cutting off part of cotyledons to expose germ growing points; (3) carrying out mutagenesis treatment by using an ethyl methanesulfonate solution as a mutagenic agent; and (4) performing rooting and germination in water, then performing field planting, and obtaining the mutant plant with phenotypic variation through phenotypic observation. A lotus EMS mutation system is explored by taking the seed lotus seeds as a material, and a result shows that shells of the lotus seeds are completely removed, so that the lotus seeds swelland germinate quickly and germinate orderly and consistently; and the shelled lotus seeds are soaked in clear water for 24 hours, 1/2-1/3 of the cotyledons are cut off, and the cotyledons are treatedin an EMS solution with the concentration of 0.5% for 16 hours to obtain mutant materials with various phenotypes, thereby providing a new germplasm material for seed-provided lotus breeding and effectively solving the problem of genetic stenosis of seed-provided lotus.

Description

technical field [0001] The invention relates to a method for mutagenesis of mutant plants, in particular to a method for mutagenesis of phenotypic variation mutant plants of lotus, and belongs to the field of mutagenesis of mutant plants of lotus. Background technique [0002] Lotus (Nelumbo nucifera) belongs to the Nymphaeaceae (Nymphaeaceae) Nelumbo plant, and has been cultivated in China for many years. According to the use, it can be divided into three categories, namely lotus root, lotus and ornamental lotus. Among them, seed lotus is widely used in dining tables, food deep processing and medicinal materials due to the same food and taste of lotus seeds. [0003] The breeding of Zilian in China started in the 1980s. The breeding methods used are mainly traditional hybridization, satellite-mounted mutagenesis and ion implantation mutagenesis. Obtaining outstanding germplasm resources is the key to seed lotus crop breeding. The improvement of breeding and production l...

AI Technical Summary

Problems solved by technology

The improvement of breeding and production level has brought opportunities and challenges to the germplasm innovation of Zilian. It is diff

Images